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Gender differences in hypertrophy, insulin resistance and ischemic injury in the aging type 2 diabetic rat heart.
J Mol Cell Cardiol. 2004 Aug; 37(2):547-55.JM

Abstract

Aging and diabetes in women increase their susceptibility to myocardial ischemic injury, but the cellular mechanisms involved are not understood. Consequently, we studied the influence of gender on cardiac insulin resistance and ischemic injury in the aging of Goto-Kakizaki (GK) rat, a model of type 2 diabetes. Male and female GK rats had heart/body weight ratios 29% (P < 0.0001) and 53% (P < 0.0001) higher, respectively, than their sex-matched controls, with the female GK rat hearts significantly more hypertrophied than the male (P < 0.001). Glucose transporter (GLUT) 1 protein levels were the same in all hearts, but GLUT4 protein levels were 28% lower (P < 0.01) in all GK rat hearts compared with their sex-matched controls. In isolated, perfused hearts, insulin-stimulated (3)H-glucose uptake rates were decreased by 23% (P < 0.05) and 40% (P < 0.05) in male and female GK rat hearts, respectively, compared with their controls, with the female significantly more insulin resistant than the male GK rat hearts (P < 0.05). Protein kinase B protein levels and insulin-stimulated phosphorylation were the same in all hearts. During low-flow ischemia, glucose uptake was 59% lower (P < 0.001) in female, but the same as controls in male, GK rat hearts. Consequently, recovery of contractile function during reperfusion was 30% lower (P < 0.05) in female, but the same as controls in male GK rat hearts. We conclude that the aging female type 2 diabetic rat heart has increased insulin resistance and greater susceptibility to ischemic injury, than non-diabetic or male type 2 diabetic rat hearts.

Authors+Show Affiliations

University Laboratory of Physiology, University of Oxford, Oxford, OX1 3PT, England, UK. Martine.Desrois@medecine.univ-mrs.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15276024

Citation

Desrois, Martine, et al. "Gender Differences in Hypertrophy, Insulin Resistance and Ischemic Injury in the Aging Type 2 Diabetic Rat Heart." Journal of Molecular and Cellular Cardiology, vol. 37, no. 2, 2004, pp. 547-55.
Desrois M, Sidell RJ, Gauguier D, et al. Gender differences in hypertrophy, insulin resistance and ischemic injury in the aging type 2 diabetic rat heart. J Mol Cell Cardiol. 2004;37(2):547-55.
Desrois, M., Sidell, R. J., Gauguier, D., Davey, C. L., Radda, G. K., & Clarke, K. (2004). Gender differences in hypertrophy, insulin resistance and ischemic injury in the aging type 2 diabetic rat heart. Journal of Molecular and Cellular Cardiology, 37(2), 547-55.
Desrois M, et al. Gender Differences in Hypertrophy, Insulin Resistance and Ischemic Injury in the Aging Type 2 Diabetic Rat Heart. J Mol Cell Cardiol. 2004;37(2):547-55. PubMed PMID: 15276024.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gender differences in hypertrophy, insulin resistance and ischemic injury in the aging type 2 diabetic rat heart. AU - Desrois,Martine, AU - Sidell,Robert J, AU - Gauguier,Dominique, AU - Davey,Carol L, AU - Radda,George K, AU - Clarke,Kieran, PY - 2003/12/23/received PY - 2004/05/13/revised PY - 2004/05/18/accepted PY - 2004/7/28/pubmed PY - 2005/1/27/medline PY - 2004/7/28/entrez SP - 547 EP - 55 JF - Journal of molecular and cellular cardiology JO - J Mol Cell Cardiol VL - 37 IS - 2 N2 - Aging and diabetes in women increase their susceptibility to myocardial ischemic injury, but the cellular mechanisms involved are not understood. Consequently, we studied the influence of gender on cardiac insulin resistance and ischemic injury in the aging of Goto-Kakizaki (GK) rat, a model of type 2 diabetes. Male and female GK rats had heart/body weight ratios 29% (P < 0.0001) and 53% (P < 0.0001) higher, respectively, than their sex-matched controls, with the female GK rat hearts significantly more hypertrophied than the male (P < 0.001). Glucose transporter (GLUT) 1 protein levels were the same in all hearts, but GLUT4 protein levels were 28% lower (P < 0.01) in all GK rat hearts compared with their sex-matched controls. In isolated, perfused hearts, insulin-stimulated (3)H-glucose uptake rates were decreased by 23% (P < 0.05) and 40% (P < 0.05) in male and female GK rat hearts, respectively, compared with their controls, with the female significantly more insulin resistant than the male GK rat hearts (P < 0.05). Protein kinase B protein levels and insulin-stimulated phosphorylation were the same in all hearts. During low-flow ischemia, glucose uptake was 59% lower (P < 0.001) in female, but the same as controls in male, GK rat hearts. Consequently, recovery of contractile function during reperfusion was 30% lower (P < 0.05) in female, but the same as controls in male GK rat hearts. We conclude that the aging female type 2 diabetic rat heart has increased insulin resistance and greater susceptibility to ischemic injury, than non-diabetic or male type 2 diabetic rat hearts. SN - 0022-2828 UR - https://www.unboundmedicine.com/medline/citation/15276024/Gender_differences_in_hypertrophy_insulin_resistance_and_ischemic_injury_in_the_aging_type_2_diabetic_rat_heart_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022282804001555 DB - PRIME DP - Unbound Medicine ER -