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The neuronal glycine transporter 2 interacts with the PDZ domain protein syntenin-1.
Mol Cell Neurosci 2004; 26(4):518-29MC

Abstract

The glycine transporter subtype 2 (GlyT2) is localized at glycinergic axon terminals where it mediates the re-uptake of glycine from the extracellular space. In this study, we used the yeast two-hybrid system to search for proteins that interact with the cytoplasmic carboxy terminal tail region of GlyT2. Screening of a rat brain cDNA library identified the PDZ domain protein syntenin-1 as an intracellular binding partner of GlyT2. In pull-down experiments, the interaction between GlyT2 and syntenin-1 was found to involve the C-terminal amino acid residues of GlyT2 and the PDZ2 domain of syntenin-1. Syntenin-1 is widely expressed in brain and co-localizes with GlyT2 in brainstem sections. Furthermore, syntenin-1 binds syntaxin 1A, which is known to regulate the plasma membrane insertion of GlyT2. Thus, syntenin-1 may be an in vivo binding partner of GlyT2 that regulates its trafficking and/or presynaptic localization in glycinergic neurons.

Authors+Show Affiliations

Department of Neurochemistry, Max-Planck-Institute for Brain Research, Deutschordenstrasse 46, 60528 Frankfurt am Main, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15276154

Citation

Ohno, Koji, et al. "The Neuronal Glycine Transporter 2 Interacts With the PDZ Domain Protein Syntenin-1." Molecular and Cellular Neurosciences, vol. 26, no. 4, 2004, pp. 518-29.
Ohno K, Koroll M, El Far O, et al. The neuronal glycine transporter 2 interacts with the PDZ domain protein syntenin-1. Mol Cell Neurosci. 2004;26(4):518-29.
Ohno, K., Koroll, M., El Far, O., Scholze, P., Gomeza, J., & Betz, H. (2004). The neuronal glycine transporter 2 interacts with the PDZ domain protein syntenin-1. Molecular and Cellular Neurosciences, 26(4), pp. 518-29.
Ohno K, et al. The Neuronal Glycine Transporter 2 Interacts With the PDZ Domain Protein Syntenin-1. Mol Cell Neurosci. 2004;26(4):518-29. PubMed PMID: 15276154.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The neuronal glycine transporter 2 interacts with the PDZ domain protein syntenin-1. AU - Ohno,Koji, AU - Koroll,Michael, AU - El Far,Oussama, AU - Scholze,Petra, AU - Gomeza,Jesus, AU - Betz,Heinrich, PY - 2004/02/11/received PY - 2004/04/08/revised PY - 2004/04/14/accepted PY - 2004/7/28/pubmed PY - 2004/11/13/medline PY - 2004/7/28/entrez SP - 518 EP - 29 JF - Molecular and cellular neurosciences JO - Mol. Cell. Neurosci. VL - 26 IS - 4 N2 - The glycine transporter subtype 2 (GlyT2) is localized at glycinergic axon terminals where it mediates the re-uptake of glycine from the extracellular space. In this study, we used the yeast two-hybrid system to search for proteins that interact with the cytoplasmic carboxy terminal tail region of GlyT2. Screening of a rat brain cDNA library identified the PDZ domain protein syntenin-1 as an intracellular binding partner of GlyT2. In pull-down experiments, the interaction between GlyT2 and syntenin-1 was found to involve the C-terminal amino acid residues of GlyT2 and the PDZ2 domain of syntenin-1. Syntenin-1 is widely expressed in brain and co-localizes with GlyT2 in brainstem sections. Furthermore, syntenin-1 binds syntaxin 1A, which is known to regulate the plasma membrane insertion of GlyT2. Thus, syntenin-1 may be an in vivo binding partner of GlyT2 that regulates its trafficking and/or presynaptic localization in glycinergic neurons. SN - 1044-7431 UR - https://www.unboundmedicine.com/medline/citation/15276154/The_neuronal_glycine_transporter_2_interacts_with_the_PDZ_domain_protein_syntenin_1_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1044743104000910 DB - PRIME DP - Unbound Medicine ER -