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Expression of apoptosis markers in the retinas of human subjects with diabetes.
Invest Ophthalmol Vis Sci. 2004 Aug; 45(8):2760-6.IO

Abstract

PURPOSE

To investigate the expression of the apoptotic mediators in the retinas from human subjects with diabetes mellitus.

METHODS

Ten donor eyes from five subjects with diabetes mellitus, and eight eyes from four nondiabetic subjects without known ocular disease serving as control subjects were examined. Immunohistochemical techniques were used with antibodies directed against glial fibrillary acidic protein (GFAP), caspase-3, Fas, Fas ligand (FasL), Bax, Bcl-2, survivin, p53, extracellular signal-regulated kinases (ERK1/2), and p38.

RESULTS

In retinas from all subjects without diabetes, weak Bcl-2 immunoreactivity was confined to GFAP-positive glial cells in the nerve fiber layer. Weak immunoreactivity for ERK1/2 was noted in a few nuclei in the inner nuclear layer and in a few Müller cell processes. Cytoplasmic immunostaining for survivin was noted in the retinal pigment epithelial cells. There was no immunoreactivity for the other antibodies tested. All diabetic retinas showed cytoplasmic immunoreactivity for caspase-3, Fas, and Bax in ganglion cells. FasL immunoreactivity was detected in GFAP-positive cells. Upregulation of Bcl-2 immunoreactivity was noted in GFAP-positive cells in nerve fiber and ganglion cell layers, and Bcl-2 induction was noted in Müller cell processes. Strong immunoreactivity for ERK1/2 was observed in many nuclei in the inner nuclear layer in GFAP-positive cells in the nerve fiber and ganglion cell layers and numerous Müller cell processes. Survivin immunoreactivity was not altered in the diabetic retinas. There was no immunoreactivity for p53 and p38.

CONCLUSIONS

Ganglion cells in diabetic retinas express several proapoptosis molecules, suggesting that these cells are the most vulnerable population. Glial cells in diabetic retinas are activated and express several antiapoptosis molecules in addition to the cytotoxic effector molecule FasL, suggesting a possible role of glial cells in induction of apoptosis in ganglion cells.

Authors+Show Affiliations

Department of Ophthalmology, College of Medicine, King Saud University, Riyadh, Saudi Arabia. abuasrar@ksu.edu.saNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15277502

Citation

Abu-El-Asrar, Ahmed M., et al. "Expression of Apoptosis Markers in the Retinas of Human Subjects With Diabetes." Investigative Ophthalmology & Visual Science, vol. 45, no. 8, 2004, pp. 2760-6.
Abu-El-Asrar AM, Dralands L, Missotten L, et al. Expression of apoptosis markers in the retinas of human subjects with diabetes. Invest Ophthalmol Vis Sci. 2004;45(8):2760-6.
Abu-El-Asrar, A. M., Dralands, L., Missotten, L., Al-Jadaan, I. A., & Geboes, K. (2004). Expression of apoptosis markers in the retinas of human subjects with diabetes. Investigative Ophthalmology & Visual Science, 45(8), 2760-6.
Abu-El-Asrar AM, et al. Expression of Apoptosis Markers in the Retinas of Human Subjects With Diabetes. Invest Ophthalmol Vis Sci. 2004;45(8):2760-6. PubMed PMID: 15277502.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression of apoptosis markers in the retinas of human subjects with diabetes. AU - Abu-El-Asrar,Ahmed M, AU - Dralands,Lieve, AU - Missotten,Luc, AU - Al-Jadaan,Ibrahim A, AU - Geboes,Karel, PY - 2004/7/28/pubmed PY - 2004/9/1/medline PY - 2004/7/28/entrez SP - 2760 EP - 6 JF - Investigative ophthalmology & visual science JO - Invest Ophthalmol Vis Sci VL - 45 IS - 8 N2 - PURPOSE: To investigate the expression of the apoptotic mediators in the retinas from human subjects with diabetes mellitus. METHODS: Ten donor eyes from five subjects with diabetes mellitus, and eight eyes from four nondiabetic subjects without known ocular disease serving as control subjects were examined. Immunohistochemical techniques were used with antibodies directed against glial fibrillary acidic protein (GFAP), caspase-3, Fas, Fas ligand (FasL), Bax, Bcl-2, survivin, p53, extracellular signal-regulated kinases (ERK1/2), and p38. RESULTS: In retinas from all subjects without diabetes, weak Bcl-2 immunoreactivity was confined to GFAP-positive glial cells in the nerve fiber layer. Weak immunoreactivity for ERK1/2 was noted in a few nuclei in the inner nuclear layer and in a few Müller cell processes. Cytoplasmic immunostaining for survivin was noted in the retinal pigment epithelial cells. There was no immunoreactivity for the other antibodies tested. All diabetic retinas showed cytoplasmic immunoreactivity for caspase-3, Fas, and Bax in ganglion cells. FasL immunoreactivity was detected in GFAP-positive cells. Upregulation of Bcl-2 immunoreactivity was noted in GFAP-positive cells in nerve fiber and ganglion cell layers, and Bcl-2 induction was noted in Müller cell processes. Strong immunoreactivity for ERK1/2 was observed in many nuclei in the inner nuclear layer in GFAP-positive cells in the nerve fiber and ganglion cell layers and numerous Müller cell processes. Survivin immunoreactivity was not altered in the diabetic retinas. There was no immunoreactivity for p53 and p38. CONCLUSIONS: Ganglion cells in diabetic retinas express several proapoptosis molecules, suggesting that these cells are the most vulnerable population. Glial cells in diabetic retinas are activated and express several antiapoptosis molecules in addition to the cytotoxic effector molecule FasL, suggesting a possible role of glial cells in induction of apoptosis in ganglion cells. SN - 0146-0404 UR - https://www.unboundmedicine.com/medline/citation/15277502/Expression_of_apoptosis_markers_in_the_retinas_of_human_subjects_with_diabetes_ DB - PRIME DP - Unbound Medicine ER -