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Muscle satellite cells adopt divergent fates: a mechanism for self-renewal?
J Cell Biol. 2004 Aug 02; 166(3):347-57.JC

Abstract

Growth, repair, and regeneration of adult skeletal muscle depends on the persistence of satellite cells: muscle stem cells resident beneath the basal lamina that surrounds each myofiber. However, how the satellite cell compartment is maintained is unclear. Here, we use cultured myofibers to model muscle regeneration and show that satellite cells adopt divergent fates. Quiescent satellite cells are synchronously activated to coexpress the transcription factors Pax7 and MyoD. Most then proliferate, down-regulate Pax7, and differentiate. In contrast, other proliferating cells maintain Pax7 but lose MyoD and withdraw from immediate differentiation. These cells are typically located in clusters, together with Pax7-ve progeny destined for differentiation. Some of the Pax7+ve/MyoD-ve cells then leave the cell cycle, thus regaining the quiescent satellite cell phenotype. Significantly, noncycling cells contained within a cluster can be stimulated to proliferate again. These observations suggest that satellite cells either differentiate or switch from terminal myogenesis to maintain the satellite cell pool.

Authors+Show Affiliations

Muscle Cell Biology Group, Medical Research Council Clinical Sciences Centre, Faculty of Medicine, Imperial College, Hammersmith Hospital Campus, Du Cane Rd., London W12 0NN, UK. peter.zammit@csc.mrc.ac.ukNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15277541

Citation

Zammit, Peter S., et al. "Muscle Satellite Cells Adopt Divergent Fates: a Mechanism for Self-renewal?" The Journal of Cell Biology, vol. 166, no. 3, 2004, pp. 347-57.
Zammit PS, Golding JP, Nagata Y, et al. Muscle satellite cells adopt divergent fates: a mechanism for self-renewal? J Cell Biol. 2004;166(3):347-57.
Zammit, P. S., Golding, J. P., Nagata, Y., Hudon, V., Partridge, T. A., & Beauchamp, J. R. (2004). Muscle satellite cells adopt divergent fates: a mechanism for self-renewal? The Journal of Cell Biology, 166(3), 347-57.
Zammit PS, et al. Muscle Satellite Cells Adopt Divergent Fates: a Mechanism for Self-renewal. J Cell Biol. 2004 Aug 2;166(3):347-57. PubMed PMID: 15277541.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Muscle satellite cells adopt divergent fates: a mechanism for self-renewal? AU - Zammit,Peter S, AU - Golding,Jon P, AU - Nagata,Yosuke, AU - Hudon,Valérie, AU - Partridge,Terence A, AU - Beauchamp,Jonathan R, Y1 - 2004/07/26/ PY - 2004/7/28/pubmed PY - 2004/9/2/medline PY - 2004/7/28/entrez SP - 347 EP - 57 JF - The Journal of cell biology JO - J Cell Biol VL - 166 IS - 3 N2 - Growth, repair, and regeneration of adult skeletal muscle depends on the persistence of satellite cells: muscle stem cells resident beneath the basal lamina that surrounds each myofiber. However, how the satellite cell compartment is maintained is unclear. Here, we use cultured myofibers to model muscle regeneration and show that satellite cells adopt divergent fates. Quiescent satellite cells are synchronously activated to coexpress the transcription factors Pax7 and MyoD. Most then proliferate, down-regulate Pax7, and differentiate. In contrast, other proliferating cells maintain Pax7 but lose MyoD and withdraw from immediate differentiation. These cells are typically located in clusters, together with Pax7-ve progeny destined for differentiation. Some of the Pax7+ve/MyoD-ve cells then leave the cell cycle, thus regaining the quiescent satellite cell phenotype. Significantly, noncycling cells contained within a cluster can be stimulated to proliferate again. These observations suggest that satellite cells either differentiate or switch from terminal myogenesis to maintain the satellite cell pool. SN - 0021-9525 UR - https://www.unboundmedicine.com/medline/citation/15277541/Muscle_satellite_cells_adopt_divergent_fates:_a_mechanism_for_self_renewal L2 - https://rupress.org/jcb/article-lookup/doi/10.1083/jcb.200312007 DB - PRIME DP - Unbound Medicine ER -