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In vivo studies of sickle red blood cells.

Abstract

The defining clinical feature of sickle cell anemia is periodic occurrence of painful vasoocclusive crisis. Factors that promote trapping and sickling of red cells in the microcirculation are likely to trigger vasoocclusion. The marked red cell heterogeneity in sickle blood and abnormal adhesion of sickle red cells to vascular endothelium would be major disruptive influences. Using ex vivo and in vivo models, the authors show how to dissect the relative contribution of heterogeneous sickle red cell classes to adhesive and obstructive events. These studies revealed that (1) both rheological abnormalities and adhesion of sickle red cells contribute to their abnormal hemodynamic behavior, (2) venules are the sites of sickle cell adhesion, and (3) sickle red cell deformability plays an important role in adhesive and obstructive events. Preferential adhesion of deformable sickle red cells in postcapillary venules followed by selective trapping of dense sickle red cells could result in vasoocclusion. An updated version of this 2-step model is presented. The multifactorial nature of sickle red cell adhesion needs to be considered in designing antiadhesive therapy in vivo.

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  • Authors+Show Affiliations

    ,

    Department of Medicine, Albert Einstein College of Medicine, Bronx, New York 10461, USA. kaul@aecom.yu.edu

    Source

    MeSH

    Anemia, Sickle Cell
    Animals
    Cell Adhesion
    Erythrocytes, Abnormal
    Hemodynamics
    Humans
    Vascular Diseases
    Venules

    Pub Type(s)

    Journal Article
    Research Support, N.I.H., Extramural
    Research Support, Non-U.S. Gov't
    Review

    Language

    eng

    PubMed ID

    15280089

    Citation

    Kaul, Dhananjay K., and Mary E. Fabry. "In Vivo Studies of Sickle Red Blood Cells." Microcirculation (New York, N.Y. : 1994), vol. 11, no. 2, 2004, pp. 153-65.
    Kaul DK, Fabry ME. In vivo studies of sickle red blood cells. Microcirculation. 2004;11(2):153-65.
    Kaul, D. K., & Fabry, M. E. (2004). In vivo studies of sickle red blood cells. Microcirculation (New York, N.Y. : 1994), 11(2), pp. 153-65.
    Kaul DK, Fabry ME. In Vivo Studies of Sickle Red Blood Cells. Microcirculation. 2004;11(2):153-65. PubMed PMID: 15280089.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - In vivo studies of sickle red blood cells. AU - Kaul,Dhananjay K, AU - Fabry,Mary E, PY - 2004/7/29/pubmed PY - 2008/6/4/medline PY - 2004/7/29/entrez SP - 153 EP - 65 JF - Microcirculation (New York, N.Y. : 1994) JO - Microcirculation VL - 11 IS - 2 N2 - The defining clinical feature of sickle cell anemia is periodic occurrence of painful vasoocclusive crisis. Factors that promote trapping and sickling of red cells in the microcirculation are likely to trigger vasoocclusion. The marked red cell heterogeneity in sickle blood and abnormal adhesion of sickle red cells to vascular endothelium would be major disruptive influences. Using ex vivo and in vivo models, the authors show how to dissect the relative contribution of heterogeneous sickle red cell classes to adhesive and obstructive events. These studies revealed that (1) both rheological abnormalities and adhesion of sickle red cells contribute to their abnormal hemodynamic behavior, (2) venules are the sites of sickle cell adhesion, and (3) sickle red cell deformability plays an important role in adhesive and obstructive events. Preferential adhesion of deformable sickle red cells in postcapillary venules followed by selective trapping of dense sickle red cells could result in vasoocclusion. An updated version of this 2-step model is presented. The multifactorial nature of sickle red cell adhesion needs to be considered in designing antiadhesive therapy in vivo. SN - 1073-9688 UR - https://www.unboundmedicine.com/medline/citation/15280089/In_vivo_studies_of_sickle_red_blood_cells_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=1073-9688&date=2004&volume=11&issue=2&spage=153 DB - PRIME DP - Unbound Medicine ER -