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Intraepithelial lymphocytes in the villous tip: do they indicate potential coeliac disease?
J Clin Pathol. 2004 Aug; 57(8):835-9.JC

Abstract

BACKGROUND

The counting of intraepithelial lymphocytes (IELs) in the villous tips of architecturally normal small bowel biopsy specimens was proposed as a method to measure mucosal infiltration in gluten sensitive patients.

AIMS

To apply this straightforward method in duodenal biopsy specimens from patients affected by potential coeliac disease (PCD) to verify whether it can discriminate these patients from controls.

METHODS

Paraffin wax embedded duodenal sections from 11 patients affected by PCD were stained with an antihuman CD3 antibody. Sections from 19 patients affected by treated coeliac disease (TCD) and 17 patients in whom coeliac disease was excluded were stained with the same antibody to serve as controls. The slides were examined blindly. IELs/20 enterocytes in five randomly chosen villous tips were counted. Patients affected by PCD were all on a gluten containing diet. They had an architecturally normal duodenal mucosa and were positive for endomysial antibody. Both TCD and non-coeliac controls were negative for endomysial antibody.

RESULTS

The mean villous tip IEL scores were 4.6 (SD, 1.5; range, 1.4-7.8) in non-coeliac controls, 7.9 (SD, 4.0; range, 2.0-18.6) in TCD, and 9.2 (SD, 4.7; range, 5.8-21.8) in patients with PCD. The difference between PCD and non-coeliac controls was significant.

CONCLUSIONS

This is a very simple and sufficiently reliable method to count IELs. In patients with an architecturally normal duodenal mucosa, the IEL count in villous tips helps to distinguish between patients with PCD and non-coeliac controls.

Authors+Show Affiliations

Gastroenterology Unit, University of Pavia, 27100 Pavia, Italy. f.biagi@smatteo.pv.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15280404

Citation

Biagi, F, et al. "Intraepithelial Lymphocytes in the Villous Tip: Do They Indicate Potential Coeliac Disease?" Journal of Clinical Pathology, vol. 57, no. 8, 2004, pp. 835-9.
Biagi F, Luinetti O, Campanella J, et al. Intraepithelial lymphocytes in the villous tip: do they indicate potential coeliac disease? J Clin Pathol. 2004;57(8):835-9.
Biagi, F., Luinetti, O., Campanella, J., Klersy, C., Zambelli, C., Villanacci, V., Lanzini, A., & Corazza, G. R. (2004). Intraepithelial lymphocytes in the villous tip: do they indicate potential coeliac disease? Journal of Clinical Pathology, 57(8), 835-9.
Biagi F, et al. Intraepithelial Lymphocytes in the Villous Tip: Do They Indicate Potential Coeliac Disease. J Clin Pathol. 2004;57(8):835-9. PubMed PMID: 15280404.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Intraepithelial lymphocytes in the villous tip: do they indicate potential coeliac disease? AU - Biagi,F, AU - Luinetti,O, AU - Campanella,J, AU - Klersy,C, AU - Zambelli,C, AU - Villanacci,V, AU - Lanzini,A, AU - Corazza,G R, PY - 2004/7/29/pubmed PY - 2004/9/29/medline PY - 2004/7/29/entrez SP - 835 EP - 9 JF - Journal of clinical pathology JO - J Clin Pathol VL - 57 IS - 8 N2 - BACKGROUND: The counting of intraepithelial lymphocytes (IELs) in the villous tips of architecturally normal small bowel biopsy specimens was proposed as a method to measure mucosal infiltration in gluten sensitive patients. AIMS: To apply this straightforward method in duodenal biopsy specimens from patients affected by potential coeliac disease (PCD) to verify whether it can discriminate these patients from controls. METHODS: Paraffin wax embedded duodenal sections from 11 patients affected by PCD were stained with an antihuman CD3 antibody. Sections from 19 patients affected by treated coeliac disease (TCD) and 17 patients in whom coeliac disease was excluded were stained with the same antibody to serve as controls. The slides were examined blindly. IELs/20 enterocytes in five randomly chosen villous tips were counted. Patients affected by PCD were all on a gluten containing diet. They had an architecturally normal duodenal mucosa and were positive for endomysial antibody. Both TCD and non-coeliac controls were negative for endomysial antibody. RESULTS: The mean villous tip IEL scores were 4.6 (SD, 1.5; range, 1.4-7.8) in non-coeliac controls, 7.9 (SD, 4.0; range, 2.0-18.6) in TCD, and 9.2 (SD, 4.7; range, 5.8-21.8) in patients with PCD. The difference between PCD and non-coeliac controls was significant. CONCLUSIONS: This is a very simple and sufficiently reliable method to count IELs. In patients with an architecturally normal duodenal mucosa, the IEL count in villous tips helps to distinguish between patients with PCD and non-coeliac controls. SN - 0021-9746 UR - https://www.unboundmedicine.com/medline/citation/15280404/Intraepithelial_lymphocytes_in_the_villous_tip:_do_they_indicate_potential_coeliac_disease L2 - https://jcp.bmj.com/lookup/pmidlookup?view=long&pmid=15280404 DB - PRIME DP - Unbound Medicine ER -