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Stress response in yeast mRNA export factor: reversible changes in Rat8p localization are caused by ethanol stress but not heat shock.
J Cell Sci. 2004 Aug 15; 117(Pt 18):4189-97.JC

Abstract

Ethanol stress (10% v/v) causes selective mRNA export in Saccharomyces cerevisiae in a similar manner to heat shock (42 degrees C). Bulk poly(A)(+) mRNA accumulates in the nucleus, whereas heat shock protein mRNA is exported under such conditions. Here we investigated the effects of stress on mRNA export factors. In cells treated with ethanol stress, the DEAD box protein Rat8p showed a rapid and reversible change in its localization, accumulating in the nucleus. This change correlated closely with the blocking of bulk poly(A)(+) mRNA export caused by ethanol stress. We also found that the nuclear accumulation of Rat8p is caused by a defect in the Xpo1p/Crm1p exportin. Intriguingly, the localization of Rat8p did not change in heat shocked cells, suggesting that the mechanisms blocking bulk poly(A)(+) mRNA export differ for heat shock and ethanol stress. These results suggest that changes in the localization of Rat8p contribute to the selective export of mRNA in ethanol stressed cells, and also indicate differences in mRNA export between the heat shock response and ethanol stress response.

Authors+Show Affiliations

Laboratory of Molecular Microbiology, Graduate School of Agriculture, Kyoto University, Uji, Kyoto 611-0011, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15280434

Citation

Takemura, Reiko, et al. "Stress Response in Yeast mRNA Export Factor: Reversible Changes in Rat8p Localization Are Caused By Ethanol Stress but Not Heat Shock." Journal of Cell Science, vol. 117, no. Pt 18, 2004, pp. 4189-97.
Takemura R, Inoue Y, Izawa S. Stress response in yeast mRNA export factor: reversible changes in Rat8p localization are caused by ethanol stress but not heat shock. J Cell Sci. 2004;117(Pt 18):4189-97.
Takemura, R., Inoue, Y., & Izawa, S. (2004). Stress response in yeast mRNA export factor: reversible changes in Rat8p localization are caused by ethanol stress but not heat shock. Journal of Cell Science, 117(Pt 18), 4189-97.
Takemura R, Inoue Y, Izawa S. Stress Response in Yeast mRNA Export Factor: Reversible Changes in Rat8p Localization Are Caused By Ethanol Stress but Not Heat Shock. J Cell Sci. 2004 Aug 15;117(Pt 18):4189-97. PubMed PMID: 15280434.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Stress response in yeast mRNA export factor: reversible changes in Rat8p localization are caused by ethanol stress but not heat shock. AU - Takemura,Reiko, AU - Inoue,Yoshiharu, AU - Izawa,Shingo, Y1 - 2004/07/27/ PY - 2004/7/29/pubmed PY - 2005/4/27/medline PY - 2004/7/29/entrez SP - 4189 EP - 97 JF - Journal of cell science JO - J Cell Sci VL - 117 IS - Pt 18 N2 - Ethanol stress (10% v/v) causes selective mRNA export in Saccharomyces cerevisiae in a similar manner to heat shock (42 degrees C). Bulk poly(A)(+) mRNA accumulates in the nucleus, whereas heat shock protein mRNA is exported under such conditions. Here we investigated the effects of stress on mRNA export factors. In cells treated with ethanol stress, the DEAD box protein Rat8p showed a rapid and reversible change in its localization, accumulating in the nucleus. This change correlated closely with the blocking of bulk poly(A)(+) mRNA export caused by ethanol stress. We also found that the nuclear accumulation of Rat8p is caused by a defect in the Xpo1p/Crm1p exportin. Intriguingly, the localization of Rat8p did not change in heat shocked cells, suggesting that the mechanisms blocking bulk poly(A)(+) mRNA export differ for heat shock and ethanol stress. These results suggest that changes in the localization of Rat8p contribute to the selective export of mRNA in ethanol stressed cells, and also indicate differences in mRNA export between the heat shock response and ethanol stress response. SN - 0021-9533 UR - https://www.unboundmedicine.com/medline/citation/15280434/Stress_response_in_yeast_mRNA_export_factor:_reversible_changes_in_Rat8p_localization_are_caused_by_ethanol_stress_but_not_heat_shock_ L2 - https://journals.biologists.com/jcs/article-lookup/doi/10.1242/jcs.01296 DB - PRIME DP - Unbound Medicine ER -