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Th1 cytokine pattern (IL-12 and IL-18) in bronchoalveolar lavage fluid (BALF) before and after treatment with interferon gamma-1b (IFN-gamma-1b) or colchicine in patients with idiopathic pulmonary fibrosis (IPF/UIP).
Sarcoidosis Vasc Diffuse Lung Dis. 2004 Jun; 21(2):105-10.SV

Abstract

BACKGROUND AND AIM

Characterization of the biologic effects of Th1 cytokines will enhance the understanding of idiopathic pulmonary fibrosis (IPF) pathogenesis and treatment selection. Th1 response is characterized by increased expression of IFN-gamma, interleukin (IL)-12 and IL-18. The present study aims to evaluate the role of Th1 cytokines and their possible changes in the bronchoalveolar lavage fluid (BALF), before and after treatment with IFN-gamma-1b or colchicine.

PATIENTS AND METHODS

We studied prospectively 10 patients (8 male, 2 female) of median age 67 yr with histologically confirmed IPF/UIP. Patients were randomly assigned to receive either IFN-gamma-1b 200 microg sc (5 patients) or colchicine 1 mg qd (5 patients) plus prednisone 10 mg qd. BALF IL-12 and IL-18 levels were measured before and after treatment.

RESULTS

BALF IL-12 levels before and after treatment did not differ significantly between the two treatment groups. However, BALF IL-18 levels were significantly decreased after treatment with IFN-gamma-1b (mean +/- SD, 58.4 +/- 15.6 pg/mL vs 42.8 +/- 4.90 pg/mL, p < 0.05). A significant difference was also found after treatment with colchicine (mean +/- SD, 66.8 +/- 36.9 pg/mL vs 42.6 +/- 1.08 pg/mL, p < 0.01). A significant correlation was found between IL-18 BALF levels and the BALF neutrophils (r = 0.75, p = 0.024).

CONCLUSION

Our data suggest the potential role of IL-18 as an inflammatory marker in the pathogenetic pathway of IPF such as its possible downregulation by IFN-gamma-1b treatment. Further studies are needed in a higher number of patients in order to define the precise role of both cytokines during the immunoregulatory response with IFN-gamma-1b.

Authors+Show Affiliations

Interstitial Lung Disease Unit, Department Pneumonology, Medical School, University of Crete, Greece.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Clinical Trial
Comparative Study
Journal Article
Randomized Controlled Trial

Language

eng

PubMed ID

15281431

Citation

Antoniou, Katerina M., et al. "Th1 Cytokine Pattern (IL-12 and IL-18) in Bronchoalveolar Lavage Fluid (BALF) Before and After Treatment With Interferon Gamma-1b (IFN-gamma-1b) or Colchicine in Patients With Idiopathic Pulmonary Fibrosis (IPF/UIP)." Sarcoidosis, Vasculitis, and Diffuse Lung Diseases : Official Journal of WASOG, vol. 21, no. 2, 2004, pp. 105-10.
Antoniou KM, Alexandrakis MG, Sfiridaki K, et al. Th1 cytokine pattern (IL-12 and IL-18) in bronchoalveolar lavage fluid (BALF) before and after treatment with interferon gamma-1b (IFN-gamma-1b) or colchicine in patients with idiopathic pulmonary fibrosis (IPF/UIP). Sarcoidosis Vasc Diffuse Lung Dis. 2004;21(2):105-10.
Antoniou, K. M., Alexandrakis, M. G., Sfiridaki, K., Tsiligianni, I., Perisinakis, K., Tzortzaki, E. G., Siafakas, N. M., & Bouros, D. E. (2004). Th1 cytokine pattern (IL-12 and IL-18) in bronchoalveolar lavage fluid (BALF) before and after treatment with interferon gamma-1b (IFN-gamma-1b) or colchicine in patients with idiopathic pulmonary fibrosis (IPF/UIP). Sarcoidosis, Vasculitis, and Diffuse Lung Diseases : Official Journal of WASOG, 21(2), 105-10.
Antoniou KM, et al. Th1 Cytokine Pattern (IL-12 and IL-18) in Bronchoalveolar Lavage Fluid (BALF) Before and After Treatment With Interferon Gamma-1b (IFN-gamma-1b) or Colchicine in Patients With Idiopathic Pulmonary Fibrosis (IPF/UIP). Sarcoidosis Vasc Diffuse Lung Dis. 2004;21(2):105-10. PubMed PMID: 15281431.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Th1 cytokine pattern (IL-12 and IL-18) in bronchoalveolar lavage fluid (BALF) before and after treatment with interferon gamma-1b (IFN-gamma-1b) or colchicine in patients with idiopathic pulmonary fibrosis (IPF/UIP). AU - Antoniou,Katerina M, AU - Alexandrakis,Michael G, AU - Sfiridaki,Katerina, AU - Tsiligianni,Ioanna, AU - Perisinakis,Kostas, AU - Tzortzaki,Eleni G, AU - Siafakas,Nikolaos M, AU - Bouros,Demosthenes E, PY - 2004/7/30/pubmed PY - 2004/12/16/medline PY - 2004/7/30/entrez SP - 105 EP - 10 JF - Sarcoidosis, vasculitis, and diffuse lung diseases : official journal of WASOG JO - Sarcoidosis Vasc Diffuse Lung Dis VL - 21 IS - 2 N2 - BACKGROUND AND AIM: Characterization of the biologic effects of Th1 cytokines will enhance the understanding of idiopathic pulmonary fibrosis (IPF) pathogenesis and treatment selection. Th1 response is characterized by increased expression of IFN-gamma, interleukin (IL)-12 and IL-18. The present study aims to evaluate the role of Th1 cytokines and their possible changes in the bronchoalveolar lavage fluid (BALF), before and after treatment with IFN-gamma-1b or colchicine. PATIENTS AND METHODS: We studied prospectively 10 patients (8 male, 2 female) of median age 67 yr with histologically confirmed IPF/UIP. Patients were randomly assigned to receive either IFN-gamma-1b 200 microg sc (5 patients) or colchicine 1 mg qd (5 patients) plus prednisone 10 mg qd. BALF IL-12 and IL-18 levels were measured before and after treatment. RESULTS: BALF IL-12 levels before and after treatment did not differ significantly between the two treatment groups. However, BALF IL-18 levels were significantly decreased after treatment with IFN-gamma-1b (mean +/- SD, 58.4 +/- 15.6 pg/mL vs 42.8 +/- 4.90 pg/mL, p < 0.05). A significant difference was also found after treatment with colchicine (mean +/- SD, 66.8 +/- 36.9 pg/mL vs 42.6 +/- 1.08 pg/mL, p < 0.01). A significant correlation was found between IL-18 BALF levels and the BALF neutrophils (r = 0.75, p = 0.024). CONCLUSION: Our data suggest the potential role of IL-18 as an inflammatory marker in the pathogenetic pathway of IPF such as its possible downregulation by IFN-gamma-1b treatment. Further studies are needed in a higher number of patients in order to define the precise role of both cytokines during the immunoregulatory response with IFN-gamma-1b. SN - 1124-0490 UR - https://www.unboundmedicine.com/medline/citation/15281431/Th1_cytokine_pattern__IL_12_and_IL_18__in_bronchoalveolar_lavage_fluid__BALF__before_and_after_treatment_with_interferon_gamma_1b__IFN_gamma_1b__or_colchicine_in_patients_with_idiopathic_pulmonary_fibrosis__IPF/UIP__ L2 - http://www.diseaseinfosearch.org/result/6087 DB - PRIME DP - Unbound Medicine ER -