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Determination of amphetamine in rat brain by in vivo microdialysis and ion-pairing liquid chromatography with electrospray tandem mass spectrometry.
Rapid Commun Mass Spectrom. 2004; 18(15):1711-4.RC

Abstract

An ion-pairing liquid chromatography/electrospray tandem mass spectrometry (LC/ES-MS/MS) method with in vivo microdialysis for the determination of amphetamine in rat brain has been developed. A microdialysis probe was surgically implanted into the striatum of the rat and artificial cerebrospinal fluid (aCSF) was used as the perfusion medium. Samples were collected and then analyzed off-line by LC/ES-MS/MS. A reversed-phase C18 column was employed for LC separation and MS/MS was utilized for detection. Trifluoroacetic acid (TFA) was added to the mobile phase (acetonitrile/water) as an ion-pairing reagent. Detection was by ES-MS/MS directly, and no post-column addition of organic modifier was needed. Dual linear ranges were determined from 0.1-0.5 microg/mL and 0.005-0.1 microg/mL, respectively. The detection limit, based on a signal-to-noise ratio of 3, was 0.001 microg/mL (5 nM). Good precision and accuracy were obtained. The applicability of this newly developed method was demonstrated by continuous monitoring of amphetamine concentrations in rat brain. Amphetamine reached a maximum concentration of 0.086 +/- 0.017 microg/mL over 20-40 min after a single 3.0 mg/kg intraperitoneal administration.

Authors+Show Affiliations

Department of Chemistry, Soochow University, Taipei, Taiwan. msfuh@mail.scu.edu.twNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15282769

Citation

Fuh, Ming-Ren, et al. "Determination of Amphetamine in Rat Brain By in Vivo Microdialysis and Ion-pairing Liquid Chromatography With Electrospray Tandem Mass Spectrometry." Rapid Communications in Mass Spectrometry : RCM, vol. 18, no. 15, 2004, pp. 1711-4.
Fuh MR, Haung CH, Wu TY, et al. Determination of amphetamine in rat brain by in vivo microdialysis and ion-pairing liquid chromatography with electrospray tandem mass spectrometry. Rapid Commun Mass Spectrom. 2004;18(15):1711-4.
Fuh, M. R., Haung, C. H., Wu, T. Y., Lin, S. L., & Pan, W. H. (2004). Determination of amphetamine in rat brain by in vivo microdialysis and ion-pairing liquid chromatography with electrospray tandem mass spectrometry. Rapid Communications in Mass Spectrometry : RCM, 18(15), 1711-4.
Fuh MR, et al. Determination of Amphetamine in Rat Brain By in Vivo Microdialysis and Ion-pairing Liquid Chromatography With Electrospray Tandem Mass Spectrometry. Rapid Commun Mass Spectrom. 2004;18(15):1711-4. PubMed PMID: 15282769.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of amphetamine in rat brain by in vivo microdialysis and ion-pairing liquid chromatography with electrospray tandem mass spectrometry. AU - Fuh,Ming-Ren, AU - Haung,Chiuan-Hung, AU - Wu,Ti-Yu, AU - Lin,Shiang-Ling, AU - Pan,Wynn H T, PY - 2004/7/30/pubmed PY - 2004/12/16/medline PY - 2004/7/30/entrez SP - 1711 EP - 4 JF - Rapid communications in mass spectrometry : RCM JO - Rapid Commun Mass Spectrom VL - 18 IS - 15 N2 - An ion-pairing liquid chromatography/electrospray tandem mass spectrometry (LC/ES-MS/MS) method with in vivo microdialysis for the determination of amphetamine in rat brain has been developed. A microdialysis probe was surgically implanted into the striatum of the rat and artificial cerebrospinal fluid (aCSF) was used as the perfusion medium. Samples were collected and then analyzed off-line by LC/ES-MS/MS. A reversed-phase C18 column was employed for LC separation and MS/MS was utilized for detection. Trifluoroacetic acid (TFA) was added to the mobile phase (acetonitrile/water) as an ion-pairing reagent. Detection was by ES-MS/MS directly, and no post-column addition of organic modifier was needed. Dual linear ranges were determined from 0.1-0.5 microg/mL and 0.005-0.1 microg/mL, respectively. The detection limit, based on a signal-to-noise ratio of 3, was 0.001 microg/mL (5 nM). Good precision and accuracy were obtained. The applicability of this newly developed method was demonstrated by continuous monitoring of amphetamine concentrations in rat brain. Amphetamine reached a maximum concentration of 0.086 +/- 0.017 microg/mL over 20-40 min after a single 3.0 mg/kg intraperitoneal administration. SN - 0951-4198 UR - https://www.unboundmedicine.com/medline/citation/15282769/Determination_of_amphetamine_in_rat_brain_by_in_vivo_microdialysis_and_ion_pairing_liquid_chromatography_with_electrospray_tandem_mass_spectrometry_ L2 - https://doi.org/10.1002/rcm.1543 DB - PRIME DP - Unbound Medicine ER -