TY - JOUR T1 - Simultaneous quantification of opiates, cocaine, and metabolites in hair by LC-APCI-MS/MS. AU - Scheidweiler,Karl B, AU - Huestis,Marilyn A, PY - 2004/7/31/pubmed PY - 2006/5/3/medline PY - 2004/7/31/entrez SP - 4358 EP - 63 JF - Analytical chemistry JO - Anal Chem VL - 76 IS - 15 N2 - A quantitative LC-APCI-MS/MS method for simultaneous measurement of opiates, cocaine, and metabolites in hair was developed and validated. Cocaine and opiates were extracted from pulverized hair via sonication in methanol at 37 degrees C for 3 h. Samples were cleaned up using solid-phase extraction. LC separation was achieved in 20 min with identification and quantification by selected reaction monitoring. Calibration by linear regression analysis utilized deuterated internal standards and a weighting factor of 1/x (R(2) > 0.998). Limits of quantification (LOQ) ranged from 17 to 50 pg/mg for cocaine and metabolites and were 83 pg/mg for opiates. Standard curves were linear from the LOQ to 5000 pg/mg for cocaine and metabolites, except benzoylecgonine (2500 pg/mg). Opiate standard curves were linear from the LOQ to 12500 pg/mg. Accuracy ranged from 84 to 115% for all quantitative analytes. Precision, % relative standard deviation, was less than 11.0% for all analytes. Methanolic sonication produced less than 5% hydrolysis of cocaine and 6-acetylmorphine. The method should be useful for studying cocaine and opiate distribution into hair. SN - 0003-2700 UR - https://www.unboundmedicine.com/medline/citation/15283573/Simultaneous_quantification_of_opiates_cocaine_and_metabolites_in_hair_by_LC_APCI_MS/MS_ DB - PRIME DP - Unbound Medicine ER -