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Nitric oxide-releasing sol-gel particle/polyurethane glucose biosensors.
Anal Chem. 2004 Aug 01; 76(15):4543-9.AC

Abstract

A hybrid sol-gel/polyurethane glucose biosensor that releases nitric oxide is developed and characterized. The biosensor consists of a platinum electrode coated with four polymeric membranes including the following: (1) sol-gel with immobilized glucose oxidase (GOx); (2) polyurethane to protect the enzyme; (3) NO donor-modified sol-gel particle-doped polyurethane; and (4) polyurethane. This configuration was developed due to the drastic reduction in sensitivity observed for NO donor-modified sol-gel film-based glucose sensors. For the hybrid sol-gel/polyurethane biosensor, sol-gel particles are first modified with the NO donor and then incorporated into a polyurethane layer that is coated onto the preimmobilized GOx electrode. In this manner, the GOx layer is not exposed to the harsh conditions necessary to impart NO release ability to the biosensor, and only a minimal decrease in sensitivity due to the NO release is observed. The glucose response of the NO-releasing glucose biosensor and its NO generation profiles are reported. In addition, the stability of the sol-gel particles in the supporting polyurethane membrane is discussed.

Authors+Show Affiliations

Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

15283600

Citation

Shin, Jae Ho, et al. "Nitric Oxide-releasing Sol-gel Particle/polyurethane Glucose Biosensors." Analytical Chemistry, vol. 76, no. 15, 2004, pp. 4543-9.
Shin JH, Marxer SM, Schoenfisch MH. Nitric oxide-releasing sol-gel particle/polyurethane glucose biosensors. Anal Chem. 2004;76(15):4543-9.
Shin, J. H., Marxer, S. M., & Schoenfisch, M. H. (2004). Nitric oxide-releasing sol-gel particle/polyurethane glucose biosensors. Analytical Chemistry, 76(15), 4543-9.
Shin JH, Marxer SM, Schoenfisch MH. Nitric Oxide-releasing Sol-gel Particle/polyurethane Glucose Biosensors. Anal Chem. 2004 Aug 1;76(15):4543-9. PubMed PMID: 15283600.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nitric oxide-releasing sol-gel particle/polyurethane glucose biosensors. AU - Shin,Jae Ho, AU - Marxer,Stephanie M, AU - Schoenfisch,Mark H, PY - 2004/7/31/pubmed PY - 2006/5/3/medline PY - 2004/7/31/entrez SP - 4543 EP - 9 JF - Analytical chemistry JO - Anal Chem VL - 76 IS - 15 N2 - A hybrid sol-gel/polyurethane glucose biosensor that releases nitric oxide is developed and characterized. The biosensor consists of a platinum electrode coated with four polymeric membranes including the following: (1) sol-gel with immobilized glucose oxidase (GOx); (2) polyurethane to protect the enzyme; (3) NO donor-modified sol-gel particle-doped polyurethane; and (4) polyurethane. This configuration was developed due to the drastic reduction in sensitivity observed for NO donor-modified sol-gel film-based glucose sensors. For the hybrid sol-gel/polyurethane biosensor, sol-gel particles are first modified with the NO donor and then incorporated into a polyurethane layer that is coated onto the preimmobilized GOx electrode. In this manner, the GOx layer is not exposed to the harsh conditions necessary to impart NO release ability to the biosensor, and only a minimal decrease in sensitivity due to the NO release is observed. The glucose response of the NO-releasing glucose biosensor and its NO generation profiles are reported. In addition, the stability of the sol-gel particles in the supporting polyurethane membrane is discussed. SN - 0003-2700 UR - https://www.unboundmedicine.com/medline/citation/15283600/Nitric_oxide_releasing_sol_gel_particle/polyurethane_glucose_biosensors_ L2 - https://doi.org/10.1021/ac049776z DB - PRIME DP - Unbound Medicine ER -