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Temporal changes in cerebral antioxidant enzyme activities after ischemia and reperfusion in a rat focal brain ischemia model: effect of dietary fish oil.
Brain Res Dev Brain Res. 2004 Aug 18; 152(1):11-8.BR

Abstract

This study investigated the neuroprotective effects of dietary supplementation of fish oil on both brain infarction and the activities of antioxidant enzymes. Male Sprague-Dawley rats (4-weeks old) were divided into two groups and received either a regular diet (RD) or a fish-oil-supplemented diet (FOD) for 6 weeks prior to middle cerebral artery (MCA) occlusion. The infarction volume of the brain was calculated using image analysis after staining. Antioxidant enzymes were measured before ischemia (BI), after 2 h of ischemia (AI) and after 24 h (24hR), 48 h (48hR) and after 7 days (7dR) of reperfusion. The infarction volume of the brain was significantly smaller in the FOD group than in the RD group after 24 h of reperfusion (p<0.05). Before ischemia, the levels of lipid peroxide and the glutathione peroxidase (GPx) activity were higher in the FOD group than in the RD group. During reperfusion, the catalase (CAT) activity in the FOD group remained at the preischemia level until after 48 h of reperfusion, while those in the RD group did not. The Mn-superoxide dismutase (SOD) activity and GPx activity were higher in the FOD group than in the RD group only after 2 h of ischemia. In the fatty acid analysis, the ratio of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) were higher in the FOD group than in the RD group (p<0.05). Our results demonstrate that supplementing the diet with fish oil could decrease the cerebral infarction volume following ischemia and reperfusion (I/R) partly by working directly as an antioxidant and partly by modulating antioxidant enzyme activities.

Authors+Show Affiliations

College of Nursing, Seoul National University, Youngun Dong 28, Chong ro Gu Seoul 110-799, South Korea. smi@plaza.sun.ac.krNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15283990

Citation

Choi-Kwon, Smi, et al. "Temporal Changes in Cerebral Antioxidant Enzyme Activities After Ischemia and Reperfusion in a Rat Focal Brain Ischemia Model: Effect of Dietary Fish Oil." Brain Research. Developmental Brain Research, vol. 152, no. 1, 2004, pp. 11-8.
Choi-Kwon S, Park KA, Lee HJ, et al. Temporal changes in cerebral antioxidant enzyme activities after ischemia and reperfusion in a rat focal brain ischemia model: effect of dietary fish oil. Brain Res Dev Brain Res. 2004;152(1):11-8.
Choi-Kwon, S., Park, K. A., Lee, H. J., Park, M. S., Lee, J. H., Jeon, S. E., Choe, M. A., & Park, K. C. (2004). Temporal changes in cerebral antioxidant enzyme activities after ischemia and reperfusion in a rat focal brain ischemia model: effect of dietary fish oil. Brain Research. Developmental Brain Research, 152(1), 11-8.
Choi-Kwon S, et al. Temporal Changes in Cerebral Antioxidant Enzyme Activities After Ischemia and Reperfusion in a Rat Focal Brain Ischemia Model: Effect of Dietary Fish Oil. Brain Res Dev Brain Res. 2004 Aug 18;152(1):11-8. PubMed PMID: 15283990.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Temporal changes in cerebral antioxidant enzyme activities after ischemia and reperfusion in a rat focal brain ischemia model: effect of dietary fish oil. AU - Choi-Kwon,Smi, AU - Park,Kyung-Ae, AU - Lee,Hee-Joo, AU - Park,Myung-Sook, AU - Lee,Joung-Hee, AU - Jeon,Sang-Eun, AU - Choe,Myoung-Ae, AU - Park,Kyoung-Chan, PY - 2004/05/25/accepted PY - 2004/7/31/pubmed PY - 2004/10/22/medline PY - 2004/7/31/entrez SP - 11 EP - 8 JF - Brain research. Developmental brain research JO - Brain Res Dev Brain Res VL - 152 IS - 1 N2 - This study investigated the neuroprotective effects of dietary supplementation of fish oil on both brain infarction and the activities of antioxidant enzymes. Male Sprague-Dawley rats (4-weeks old) were divided into two groups and received either a regular diet (RD) or a fish-oil-supplemented diet (FOD) for 6 weeks prior to middle cerebral artery (MCA) occlusion. The infarction volume of the brain was calculated using image analysis after staining. Antioxidant enzymes were measured before ischemia (BI), after 2 h of ischemia (AI) and after 24 h (24hR), 48 h (48hR) and after 7 days (7dR) of reperfusion. The infarction volume of the brain was significantly smaller in the FOD group than in the RD group after 24 h of reperfusion (p<0.05). Before ischemia, the levels of lipid peroxide and the glutathione peroxidase (GPx) activity were higher in the FOD group than in the RD group. During reperfusion, the catalase (CAT) activity in the FOD group remained at the preischemia level until after 48 h of reperfusion, while those in the RD group did not. The Mn-superoxide dismutase (SOD) activity and GPx activity were higher in the FOD group than in the RD group only after 2 h of ischemia. In the fatty acid analysis, the ratio of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) were higher in the FOD group than in the RD group (p<0.05). Our results demonstrate that supplementing the diet with fish oil could decrease the cerebral infarction volume following ischemia and reperfusion (I/R) partly by working directly as an antioxidant and partly by modulating antioxidant enzyme activities. SN - 0165-3806 UR - https://www.unboundmedicine.com/medline/citation/15283990/Temporal_changes_in_cerebral_antioxidant_enzyme_activities_after_ischemia_and_reperfusion_in_a_rat_focal_brain_ischemia_model:_effect_of_dietary_fish_oil_ DB - PRIME DP - Unbound Medicine ER -