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A direct repeat sequence associated with the centromeric retrotransposons in wheat.
Genome 2004; 47(4):747-56G

Abstract

A high-density BAC filter of Triticum monococcum was screened for the presence of a centromeric retrotransposon using the integrase region as a probe. Southern hybridization to the BAC digests using total genomic DNA probes of Triticum monococcum, Triticum aestivum, and Hordeum vulgare detected differentially hybridizing restriction fragments between wheat and barley. The fragments that hybridized to genomic DNA of wheat but not to that of barley were subcloned. Fluorescence in situ hybridization (FISH) analysis indicated that the clone pHind258 hybridized strongly to centromeric regions in wheat and rye and weakly to those in barley. The sequence of pHind258 was homologous to integrase and long terminal repeats of centromeric Ty3-gypsy retrotransposons of cereal species. Additionally, pHind258 has a pair of 192-bp direct repeats. FISH analysis indicated that the 192-bp repeat probe hybridized to centromeres of wheat and rye but not to those of barley. We found differential FISH signal intensities among wheat chromosomes using the 192-bp probe. In general, the A-genome chromosomes possess strong FISH signals, the B-genome chromosomes possess moderate signals, and the D-genome chromosomes possess weak signals. This was consistent with the estimated copy numbers of the 192-bp repeats in the ancestral species of hexaploid wheat.

Authors+Show Affiliations

Laboratory of Plant Genetics, Graduate School of Agriculture, Kyoto University, Kitashirakawaoiwake-cho, Sakyo-ku, Kyoto 606-8502, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15284880

Citation

Ito, Hidetaka, et al. "A Direct Repeat Sequence Associated With the Centromeric Retrotransposons in Wheat." Genome, vol. 47, no. 4, 2004, pp. 747-56.
Ito H, Nasuda S, Endo TR. A direct repeat sequence associated with the centromeric retrotransposons in wheat. Genome. 2004;47(4):747-56.
Ito, H., Nasuda, S., & Endo, T. R. (2004). A direct repeat sequence associated with the centromeric retrotransposons in wheat. Genome, 47(4), pp. 747-56.
Ito H, Nasuda S, Endo TR. A Direct Repeat Sequence Associated With the Centromeric Retrotransposons in Wheat. Genome. 2004;47(4):747-56. PubMed PMID: 15284880.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A direct repeat sequence associated with the centromeric retrotransposons in wheat. AU - Ito,Hidetaka, AU - Nasuda,Shuhei, AU - Endo,Takashi R, PY - 2004/7/31/pubmed PY - 2005/2/8/medline PY - 2004/7/31/entrez SP - 747 EP - 56 JF - Genome JO - Genome VL - 47 IS - 4 N2 - A high-density BAC filter of Triticum monococcum was screened for the presence of a centromeric retrotransposon using the integrase region as a probe. Southern hybridization to the BAC digests using total genomic DNA probes of Triticum monococcum, Triticum aestivum, and Hordeum vulgare detected differentially hybridizing restriction fragments between wheat and barley. The fragments that hybridized to genomic DNA of wheat but not to that of barley were subcloned. Fluorescence in situ hybridization (FISH) analysis indicated that the clone pHind258 hybridized strongly to centromeric regions in wheat and rye and weakly to those in barley. The sequence of pHind258 was homologous to integrase and long terminal repeats of centromeric Ty3-gypsy retrotransposons of cereal species. Additionally, pHind258 has a pair of 192-bp direct repeats. FISH analysis indicated that the 192-bp repeat probe hybridized to centromeres of wheat and rye but not to those of barley. We found differential FISH signal intensities among wheat chromosomes using the 192-bp probe. In general, the A-genome chromosomes possess strong FISH signals, the B-genome chromosomes possess moderate signals, and the D-genome chromosomes possess weak signals. This was consistent with the estimated copy numbers of the 192-bp repeats in the ancestral species of hexaploid wheat. SN - 0831-2796 UR - https://www.unboundmedicine.com/medline/citation/15284880/A_direct_repeat_sequence_associated_with_the_centromeric_retrotransposons_in_wheat_ L2 - http://www.nrcresearchpress.com/doi/full/10.1139/g04-034?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -