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Aging, gender and APOE isotype modulate metabolism of Alzheimer's Abeta peptides and F-isoprostanes in the absence of detectable amyloid deposits.

Abstract

Aging and apolipoprotein E (APOE) isoform are among the most consistent risks for the development of Alzheimer's disease (AD). Metabolic factors that modulate risk have been elusive, though oxidative reactions and their by-products have been implicated in human AD and in transgenic mice with overt histological amyloidosis. We investigated the relationship between the levels of endogenous murine amyloid beta (Abeta) peptides and the levels of a marker of oxidation in mice that never develop histological amyloidosis [i.e. APOE knockout (KO) mice with or without transgenic human APOEepsilon3 or human APOEepsilon4 alleles]. Aging-, gender-, and APOE-genotype-dependent changes were observed for endogenous mouse brain Abeta40 and Abeta42 peptides. Levels of the oxidized lipid F2-isoprostane (F2-isoPs) in the brains of the same animals as those used for the Abeta analyses revealed aging- and gender-dependent changes in APOE KO and in human APOEepsilon4 transgenic KO mice. Human APOEepsilon3 transgenic KO mice did not exhibit aging- or gender-dependent increases in F2-isoPs. In general, the changes in the levels of brain F2-isoPs in mice according to age, gender, and APOE genotype mirrored the changes in brain Abeta levels, which, in turn, paralleled known trends in the risk for human AD. These data indicate that there exists an aging-dependent, APOE-genotype-sensitive rise in murine brain Abeta levels despite the apparent inability of the peptide to form histologically detectable amyloid. Human APOEepsilon3, but not human APOEepsilon4, can apparently prevent the aging-dependent rise in murine brain Abeta levels, consistent with the relative risk for AD associated with these genotypes. The fidelity of the brain Abeta/F2-isoP relationship across multiple relevant variables supports the hypothesis that oxidized lipids play a role in AD pathogenesis, as has been suggested by recent evidence that F2-isoPs can stimulate Abeta generation and aggregation.

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  • Authors+Show Affiliations

    ,

    Department of Psychiatry, New York University, The Nathan S. Kline Institute for Psychiatric Research, Orangeburg, New York, USA.

    , , , , , , , , , , , , , , , , , ,

    Source

    Journal of neurochemistry 90:4 2004 Aug pg 1011-8

    MeSH

    Aging
    Alzheimer Disease
    Amyloid beta-Peptides
    Amyloidosis
    Animals
    Apolipoprotein E3
    Apolipoprotein E4
    Apolipoproteins E
    Astrocytes
    Cell Count
    Choline O-Acetyltransferase
    Disease Models, Animal
    Disease Progression
    F2-Isoprostanes
    Humans
    Lipid Metabolism
    Mice
    Mice, Inbred C57BL
    Mice, Knockout
    Mice, Transgenic
    Neurons
    Oxidative Stress
    Peptide Fragments
    Sex Factors

    Pub Type(s)

    Journal Article
    Research Support, U.S. Gov't, P.H.S.

    Language

    eng

    PubMed ID

    15287908

    Citation

    Yao, Jun, et al. "Aging, Gender and APOE Isotype Modulate Metabolism of Alzheimer's Abeta Peptides and F-isoprostanes in the Absence of Detectable Amyloid Deposits." Journal of Neurochemistry, vol. 90, no. 4, 2004, pp. 1011-8.
    Yao J, Petanceska SS, Montine TJ, et al. Aging, gender and APOE isotype modulate metabolism of Alzheimer's Abeta peptides and F-isoprostanes in the absence of detectable amyloid deposits. J Neurochem. 2004;90(4):1011-8.
    Yao, J., Petanceska, S. S., Montine, T. J., Holtzman, D. M., Schmidt, S. D., Parker, C. A., ... Gandy, S. (2004). Aging, gender and APOE isotype modulate metabolism of Alzheimer's Abeta peptides and F-isoprostanes in the absence of detectable amyloid deposits. Journal of Neurochemistry, 90(4), pp. 1011-8.
    Yao J, et al. Aging, Gender and APOE Isotype Modulate Metabolism of Alzheimer's Abeta Peptides and F-isoprostanes in the Absence of Detectable Amyloid Deposits. J Neurochem. 2004;90(4):1011-8. PubMed PMID: 15287908.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Aging, gender and APOE isotype modulate metabolism of Alzheimer's Abeta peptides and F-isoprostanes in the absence of detectable amyloid deposits. AU - Yao,Jun, AU - Petanceska,Suzana S, AU - Montine,Thomas J, AU - Holtzman,David M, AU - Schmidt,Stephen D, AU - Parker,Carolyn A, AU - Callahan,Michael J, AU - Lipinski,William J, AU - Bisgaier,Charles L, AU - Turner,Brian A, AU - Nixon,Ralph A, AU - Martins,Ralph N, AU - Ouimet,Charles, AU - Smith,Jonathan D, AU - Davies,Peter, AU - Laska,Eugene, AU - Ehrlich,Michelle E, AU - Walker,Lary C, AU - Mathews,Paul M, AU - Gandy,Sam, PY - 2004/8/4/pubmed PY - 2004/10/29/medline PY - 2004/8/4/entrez SP - 1011 EP - 8 JF - Journal of neurochemistry JO - J. Neurochem. VL - 90 IS - 4 N2 - Aging and apolipoprotein E (APOE) isoform are among the most consistent risks for the development of Alzheimer's disease (AD). Metabolic factors that modulate risk have been elusive, though oxidative reactions and their by-products have been implicated in human AD and in transgenic mice with overt histological amyloidosis. We investigated the relationship between the levels of endogenous murine amyloid beta (Abeta) peptides and the levels of a marker of oxidation in mice that never develop histological amyloidosis [i.e. APOE knockout (KO) mice with or without transgenic human APOEepsilon3 or human APOEepsilon4 alleles]. Aging-, gender-, and APOE-genotype-dependent changes were observed for endogenous mouse brain Abeta40 and Abeta42 peptides. Levels of the oxidized lipid F2-isoprostane (F2-isoPs) in the brains of the same animals as those used for the Abeta analyses revealed aging- and gender-dependent changes in APOE KO and in human APOEepsilon4 transgenic KO mice. Human APOEepsilon3 transgenic KO mice did not exhibit aging- or gender-dependent increases in F2-isoPs. In general, the changes in the levels of brain F2-isoPs in mice according to age, gender, and APOE genotype mirrored the changes in brain Abeta levels, which, in turn, paralleled known trends in the risk for human AD. These data indicate that there exists an aging-dependent, APOE-genotype-sensitive rise in murine brain Abeta levels despite the apparent inability of the peptide to form histologically detectable amyloid. Human APOEepsilon3, but not human APOEepsilon4, can apparently prevent the aging-dependent rise in murine brain Abeta levels, consistent with the relative risk for AD associated with these genotypes. The fidelity of the brain Abeta/F2-isoP relationship across multiple relevant variables supports the hypothesis that oxidized lipids play a role in AD pathogenesis, as has been suggested by recent evidence that F2-isoPs can stimulate Abeta generation and aggregation. SN - 0022-3042 UR - https://www.unboundmedicine.com/medline/citation/15287908/Aging_gender_and_APOE_isotype_modulate_metabolism_of_Alzheimer's_Abeta_peptides_and_F_isoprostanes_in_the_absence_of_detectable_amyloid_deposits_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0022-3042&date=2004&volume=90&issue=4&spage=1011 DB - PRIME DP - Unbound Medicine ER -