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Development of stellate and basket cells and their apoptosis in mouse cerebellar cortex.
Neurosci Res 2004; 50(1):13-22NR

Abstract

Stellate and basket cells in the molecular layer (ML) of the cerebellar cortex proliferate within the white matter (WH) during development. Developmental neuronal death has been documented on granule cells but has not been demonstrated on other GABAergic neurons. We investigated the migration and the cell death of stellate/basket cells further in glutamic acid decarboxylase 67/green fluorescent protein (GFP) knock-in mouse in which every GABAergic neuron was identified by its GFP fluorescence. Analyses were made in the first three postnatal weeks. In the WM, GFP-positive cells were abundant on postnatal day (P) 5-15 but scarce in P21. Stellate/basket cells increased in number in the ML until P15, corresponding to the growth of the ML. Administration of 5-bromo-2'deoxyuridine (BrdU) at P2-8 labeled many cells in the WM within 1h. After BrdU administration at P5, many BrdU-labeled GFP-positive cells were observed in the WM and the internal granular layer at P7, and in the ML at P9. These results support the proliferation of stellate/basket cells in the WM and their migration to the ML. Apoptosis of GABAergic interneurons was demonstrated in the ML and WM during the first two weeks. Their apoptotic loss will contribute to the adjustment of neuron number or elimination of any improper populations.

Authors+Show Affiliations

National Instituute of Physiologcal Sciences, The School of Life Science, Graduate University for Advanced Studies, Okazaki, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15288494

Citation

Yamanaka, Hajime, et al. "Development of Stellate and Basket Cells and Their Apoptosis in Mouse Cerebellar Cortex." Neuroscience Research, vol. 50, no. 1, 2004, pp. 13-22.
Yamanaka H, Yanagawa Y, Obata K. Development of stellate and basket cells and their apoptosis in mouse cerebellar cortex. Neurosci Res. 2004;50(1):13-22.
Yamanaka, H., Yanagawa, Y., & Obata, K. (2004). Development of stellate and basket cells and their apoptosis in mouse cerebellar cortex. Neuroscience Research, 50(1), pp. 13-22.
Yamanaka H, Yanagawa Y, Obata K. Development of Stellate and Basket Cells and Their Apoptosis in Mouse Cerebellar Cortex. Neurosci Res. 2004;50(1):13-22. PubMed PMID: 15288494.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of stellate and basket cells and their apoptosis in mouse cerebellar cortex. AU - Yamanaka,Hajime, AU - Yanagawa,Yuchio, AU - Obata,Kunihiko, PY - 2004/02/03/received PY - 2004/06/03/accepted PY - 2004/8/4/pubmed PY - 2004/11/2/medline PY - 2004/8/4/entrez SP - 13 EP - 22 JF - Neuroscience research JO - Neurosci. Res. VL - 50 IS - 1 N2 - Stellate and basket cells in the molecular layer (ML) of the cerebellar cortex proliferate within the white matter (WH) during development. Developmental neuronal death has been documented on granule cells but has not been demonstrated on other GABAergic neurons. We investigated the migration and the cell death of stellate/basket cells further in glutamic acid decarboxylase 67/green fluorescent protein (GFP) knock-in mouse in which every GABAergic neuron was identified by its GFP fluorescence. Analyses were made in the first three postnatal weeks. In the WM, GFP-positive cells were abundant on postnatal day (P) 5-15 but scarce in P21. Stellate/basket cells increased in number in the ML until P15, corresponding to the growth of the ML. Administration of 5-bromo-2'deoxyuridine (BrdU) at P2-8 labeled many cells in the WM within 1h. After BrdU administration at P5, many BrdU-labeled GFP-positive cells were observed in the WM and the internal granular layer at P7, and in the ML at P9. These results support the proliferation of stellate/basket cells in the WM and their migration to the ML. Apoptosis of GABAergic interneurons was demonstrated in the ML and WM during the first two weeks. Their apoptotic loss will contribute to the adjustment of neuron number or elimination of any improper populations. SN - 0168-0102 UR - https://www.unboundmedicine.com/medline/citation/15288494/Development_of_stellate_and_basket_cells_and_their_apoptosis_in_mouse_cerebellar_cortex_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168010204001361 DB - PRIME DP - Unbound Medicine ER -