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16S rDNA PCR analysis of infectious keratitis: a case series.
Acta Ophthalmol Scand. 2004 Aug; 82(4):463-7.AO

Abstract

PURPOSE

To discuss the value of polymerase chain reaction (PCR) in the management of bacterial infectious keratitis.

METHODS

Corneal scrapings of four patients with severe infectious keratitis were analysed by culture and PCR of 16S ribosomal DNA (rDNA), followed by direct sequencing of the resulting amplicon. The medical history of the patients included laser-assisted in-situ keratomileusis (LASIK), penetrating keratoplasty (PKP) and trauma.

RESULTS

Using PCR we were able to identify a possible pathogen in all four cases, while bacterial cultures were either negative or did not correspond to the clinical picture. The identified bacteria were a Pseudomonas species, Abiotrophia defectiva, Stenotrophomonas maltophilia and Porphyromonas gingivalis.

CONCLUSIONS

Analysis of corneal scrapings by 16S rDNA PCR should be considered as a supplement to standard microbiological procedures. However, the results of this relatively new method have to be interpreted carefully.

Authors+Show Affiliations

Department of Ophthalmology, Sahlgrenska University Hospital, Gothenburg University, Gothenburg, Sweden. thiemo.rudolph@web.deNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Case Reports
Journal Article

Language

eng

PubMed ID

15291943

Citation

Rudolph, Thiemo, et al. "16S rDNA PCR Analysis of Infectious Keratitis: a Case Series." Acta Ophthalmologica Scandinavica, vol. 82, no. 4, 2004, pp. 463-7.
Rudolph T, Welinder-Olsson C, Lind-Brandberg L, et al. 16S rDNA PCR analysis of infectious keratitis: a case series. Acta Ophthalmol Scand. 2004;82(4):463-7.
Rudolph, T., Welinder-Olsson, C., Lind-Brandberg, L., & Stenevi, U. (2004). 16S rDNA PCR analysis of infectious keratitis: a case series. Acta Ophthalmologica Scandinavica, 82(4), 463-7.
Rudolph T, et al. 16S rDNA PCR Analysis of Infectious Keratitis: a Case Series. Acta Ophthalmol Scand. 2004;82(4):463-7. PubMed PMID: 15291943.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - 16S rDNA PCR analysis of infectious keratitis: a case series. AU - Rudolph,Thiemo, AU - Welinder-Olsson,Christina, AU - Lind-Brandberg,Lena, AU - Stenevi,Ulf, PY - 2004/8/5/pubmed PY - 2004/10/16/medline PY - 2004/8/5/entrez SP - 463 EP - 7 JF - Acta ophthalmologica Scandinavica JO - Acta Ophthalmol Scand VL - 82 IS - 4 N2 - PURPOSE: To discuss the value of polymerase chain reaction (PCR) in the management of bacterial infectious keratitis. METHODS: Corneal scrapings of four patients with severe infectious keratitis were analysed by culture and PCR of 16S ribosomal DNA (rDNA), followed by direct sequencing of the resulting amplicon. The medical history of the patients included laser-assisted in-situ keratomileusis (LASIK), penetrating keratoplasty (PKP) and trauma. RESULTS: Using PCR we were able to identify a possible pathogen in all four cases, while bacterial cultures were either negative or did not correspond to the clinical picture. The identified bacteria were a Pseudomonas species, Abiotrophia defectiva, Stenotrophomonas maltophilia and Porphyromonas gingivalis. CONCLUSIONS: Analysis of corneal scrapings by 16S rDNA PCR should be considered as a supplement to standard microbiological procedures. However, the results of this relatively new method have to be interpreted carefully. SN - 1395-3907 UR - https://www.unboundmedicine.com/medline/citation/15291943/16S_rDNA_PCR_analysis_of_infectious_keratitis:_a_case_series_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=1395-3907&date=2004&volume=82&issue=4&spage=463 DB - PRIME DP - Unbound Medicine ER -