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[Comparison of the methenamine silver staining, direct fluorescent antibody and nested-polymerase chain reaction methods in the diagnosis of Pneumocystis carinii pneumonia].
Mikrobiyol Bul. 2004 Jan-Apr; 38(1-2):105-12.MB

Abstract

Pneumocystis carinii is one of the most common causative agents of pneumonia in immunocompromised patients, but the problems in the laboratory diagnosis of the disease frequently leads to diagnosis according to the response to medical treatment. In this study, the presence of P. carinii was investigated in immunocompromised patients who were presenting with the clinical symptoms of atypical pneumonia, by Gomori methenamine silver staining (GMS), direct fluorescent antibody (DFA) test and nested-polymerase chain reaction (nPCR) methods. Fifty-three samples of 49 patients were included in the study. Twelve of the samples (22.6%) were found to be positive by nPCR, 6 of them (11.3%) were found to be positive by DFA, while only one of them (1.8%) was positive by GMS staining method. As a result, for the appropriate treatment and prophylaxis of P. carinii infections, PCR which is a rapid and reliable diagnostic test should be used for diagnosis.

Authors+Show Affiliations

Güneş I
Gazi Universitesi Tip Fakültesi, Tibbi Mikrobiyoloji Anabilim Dali, Ankara.
Kalkanci A
No affiliation info available
Kuştimur S
No affiliation info available
Ergüven S
No affiliation info available
Ozet G
No affiliation info available
Ekim N
No affiliation info available

MeSH

DNA, FungalFluorescent Antibody Technique, DirectHumansImmunocompromised HostMethenaminePneumocystis cariniiPneumonia, PneumocystisPolymerase Chain ReactionReagent Kits, DiagnosticSilver Staining

Pub Type(s)

Comparative Study
Journal Article

Language

tur

PubMed ID

15293909

Citation

Güneş, Ilkay, et al. "[Comparison of the Methenamine Silver Staining, Direct Fluorescent Antibody and Nested-polymerase Chain Reaction Methods in the Diagnosis of Pneumocystis Carinii Pneumonia]." Mikrobiyoloji Bulteni, vol. 38, no. 1-2, 2004, pp. 105-12.
Güneş I, Kalkanci A, Kuştimur S, et al. [Comparison of the methenamine silver staining, direct fluorescent antibody and nested-polymerase chain reaction methods in the diagnosis of Pneumocystis carinii pneumonia]. Mikrobiyol Bul. 2004;38(1-2):105-12.
Güneş, I., Kalkanci, A., Kuştimur, S., Ergüven, S., Ozet, G., & Ekim, N. (2004). [Comparison of the methenamine silver staining, direct fluorescent antibody and nested-polymerase chain reaction methods in the diagnosis of Pneumocystis carinii pneumonia]. Mikrobiyoloji Bulteni, 38(1-2), 105-12.
Güneş I, et al. [Comparison of the Methenamine Silver Staining, Direct Fluorescent Antibody and Nested-polymerase Chain Reaction Methods in the Diagnosis of Pneumocystis Carinii Pneumonia]. Mikrobiyol Bul. 2004 Jan-Apr;38(1-2):105-12. PubMed PMID: 15293909.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Comparison of the methenamine silver staining, direct fluorescent antibody and nested-polymerase chain reaction methods in the diagnosis of Pneumocystis carinii pneumonia]. AU - Güneş,Ilkay, AU - Kalkanci,Ayşe, AU - Kuştimur,Semra, AU - Ergüven,Sibel, AU - Ozet,Gülsüm, AU - Ekim,Numan, PY - 2004/8/6/pubmed PY - 2005/7/22/medline PY - 2004/8/6/entrez SP - 105 EP - 12 JF - Mikrobiyoloji bulteni JO - Mikrobiyol Bul VL - 38 IS - 1-2 N2 - Pneumocystis carinii is one of the most common causative agents of pneumonia in immunocompromised patients, but the problems in the laboratory diagnosis of the disease frequently leads to diagnosis according to the response to medical treatment. In this study, the presence of P. carinii was investigated in immunocompromised patients who were presenting with the clinical symptoms of atypical pneumonia, by Gomori methenamine silver staining (GMS), direct fluorescent antibody (DFA) test and nested-polymerase chain reaction (nPCR) methods. Fifty-three samples of 49 patients were included in the study. Twelve of the samples (22.6%) were found to be positive by nPCR, 6 of them (11.3%) were found to be positive by DFA, while only one of them (1.8%) was positive by GMS staining method. As a result, for the appropriate treatment and prophylaxis of P. carinii infections, PCR which is a rapid and reliable diagnostic test should be used for diagnosis. SN - 0374-9096 UR - https://www.unboundmedicine.com/medline/citation/15293909/[Comparison_of_the_methenamine_silver_staining_direct_fluorescent_antibody_and_nested_polymerase_chain_reaction_methods_in_the_diagnosis_of_Pneumocystis_carinii_pneumonia]_ DB - PRIME DP - Unbound Medicine ER -