Tags

Type your tag names separated by a space and hit enter

Effects of arginine supplementation on mucosal immunity in rats with septic peritonitis.
Clin Nutr. 2004 Aug; 23(4):561-9.CN

Abstract

BACKGROUND

Supplemental Arginine (Arg) has been demonstrated to improve the immunologic response and reduce mortality in rodents with sepsis. However, the effects of Arg on gut-associated lymphoid tissue function after infection and sepsis are not clear. The aim of this study was to study the effect of Arg-supplemented diets before and Arg-enriched total parenteral nutrition (TPN) after sepsis or both on the intestinal immunity of rats with septic peritonitis.

METHODS

Rats were assigned to four groups. Groups 1 and 2 were fed a semipurified diet, while in the diets of groups 3 and 4, part of the casein was replaced with Arg. After feeding the experimental diets for 10 days, sepsis was induced by cecal ligation and puncture (CLP); at the same time, the internal jugular vein was cannulated. All rats were maintained on TPN for 3 days. Groups 1 and 3 were infused with conventional TPN, while groups 2 and 4 were given a TPN solution supplemented with Arg, which replaced 10% of the total amino acids. All rats were sacrificed 3 days after CLP. Intestinal immunoglobin (Ig) A levels, total lymphocyte yields, and lymphocyte subpopulations in Peyer's patches were analyzed. In vitro cytokine secretion by splenocytes and Peyer's patch lymphocytes were also measured.

RESULTS

Total lymphocyte yields in Peyer's patches, and small intestinal immunoglobulin A (IgA) secretion in group 4 were significantly higher than the groups 1 and 2. No differences were observed between groups 3 and 4. There were no differences in the interleukin (IL)-2 and interferon- gamma levels among all groups when splenocytes were stimulated with mitogen. However, in vitro splenocyte IL-10 production in group 4 was significantly higher than those of groups 1 and 2, and had no difference from group 3. There were no differences in the ratios of B and T lymphocyte subpopulations in Peyer's patches among all groups.

CONCLUSIONS

Enteral Arg supplementation before sepsis tended to enhance total lymphocyte yields in Peyer's patches and intestinal IgA secretion. Arg administered both before and after CLP had a synergistic effect on improving intestinal immunity, possibly by enhancing systemic IL-10 secretion. However, intravenous Arg administration after CLP had no favorable effects on mucosal immunity in rats with septic peritonitis.

Authors+Show Affiliations

Department of Microbiology and Immunology, Taipei Medical University, 250 Wu-Hsing Street, Taipei, Taiwan, ROC.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15297092

Citation

Shang, Huey-Fang, et al. "Effects of Arginine Supplementation On Mucosal Immunity in Rats With Septic Peritonitis." Clinical Nutrition (Edinburgh, Scotland), vol. 23, no. 4, 2004, pp. 561-9.
Shang HF, Wang YY, Lai YN, et al. Effects of arginine supplementation on mucosal immunity in rats with septic peritonitis. Clin Nutr. 2004;23(4):561-9.
Shang, H. F., Wang, Y. Y., Lai, Y. N., Chiu, W. C., & Yeh, S. L. (2004). Effects of arginine supplementation on mucosal immunity in rats with septic peritonitis. Clinical Nutrition (Edinburgh, Scotland), 23(4), 561-9.
Shang HF, et al. Effects of Arginine Supplementation On Mucosal Immunity in Rats With Septic Peritonitis. Clin Nutr. 2004;23(4):561-9. PubMed PMID: 15297092.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effects of arginine supplementation on mucosal immunity in rats with septic peritonitis. AU - Shang,Huey-Fang, AU - Wang,Yi-Yun, AU - Lai,Yu-Ni, AU - Chiu,Wan-Chun, AU - Yeh,Sung-Ling, PY - 2003/06/25/received PY - 2003/10/08/accepted PY - 2004/8/7/pubmed PY - 2005/4/13/medline PY - 2004/8/7/entrez SP - 561 EP - 9 JF - Clinical nutrition (Edinburgh, Scotland) JO - Clin Nutr VL - 23 IS - 4 N2 - BACKGROUND: Supplemental Arginine (Arg) has been demonstrated to improve the immunologic response and reduce mortality in rodents with sepsis. However, the effects of Arg on gut-associated lymphoid tissue function after infection and sepsis are not clear. The aim of this study was to study the effect of Arg-supplemented diets before and Arg-enriched total parenteral nutrition (TPN) after sepsis or both on the intestinal immunity of rats with septic peritonitis. METHODS: Rats were assigned to four groups. Groups 1 and 2 were fed a semipurified diet, while in the diets of groups 3 and 4, part of the casein was replaced with Arg. After feeding the experimental diets for 10 days, sepsis was induced by cecal ligation and puncture (CLP); at the same time, the internal jugular vein was cannulated. All rats were maintained on TPN for 3 days. Groups 1 and 3 were infused with conventional TPN, while groups 2 and 4 were given a TPN solution supplemented with Arg, which replaced 10% of the total amino acids. All rats were sacrificed 3 days after CLP. Intestinal immunoglobin (Ig) A levels, total lymphocyte yields, and lymphocyte subpopulations in Peyer's patches were analyzed. In vitro cytokine secretion by splenocytes and Peyer's patch lymphocytes were also measured. RESULTS: Total lymphocyte yields in Peyer's patches, and small intestinal immunoglobulin A (IgA) secretion in group 4 were significantly higher than the groups 1 and 2. No differences were observed between groups 3 and 4. There were no differences in the interleukin (IL)-2 and interferon- gamma levels among all groups when splenocytes were stimulated with mitogen. However, in vitro splenocyte IL-10 production in group 4 was significantly higher than those of groups 1 and 2, and had no difference from group 3. There were no differences in the ratios of B and T lymphocyte subpopulations in Peyer's patches among all groups. CONCLUSIONS: Enteral Arg supplementation before sepsis tended to enhance total lymphocyte yields in Peyer's patches and intestinal IgA secretion. Arg administered both before and after CLP had a synergistic effect on improving intestinal immunity, possibly by enhancing systemic IL-10 secretion. However, intravenous Arg administration after CLP had no favorable effects on mucosal immunity in rats with septic peritonitis. SN - 0261-5614 UR - https://www.unboundmedicine.com/medline/citation/15297092/Effects_of_arginine_supplementation_on_mucosal_immunity_in_rats_with_septic_peritonitis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0261561403002164 DB - PRIME DP - Unbound Medicine ER -