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[Determination of anti-transglutaminase antibodies in the diagnosis of coeliac disease in children: results of a five year prospective study].
Ann Biol Clin (Paris) 2004 Jul-Aug; 62(4):431-6AB

Abstract

AIM

To evaluate the interest of IgA antibodies to tissue transglutaminase in the diagnosis of children coeliac disease compared with anti-endomysium and anti-gliadin antibodies.

SUBJECTS AND METHODS

Seventy children with coeliac disease (mean age: 5 years and 8 months) and 99 disease controls (mean age: 4 years and 5 months). IgA anti-transglutaminase were tested by ELISA using a human recombinant tissue transglutaminase. IgA anti-endomysium were detected by indirect immunofluorescence on monkey oesophagus.

RESULTS

The middle rate of IgA anti-transglutaminase was 101.06 units in patients and only 0.47 unit in controls. IgA anti-transglutaminase and IgA anti-endomysium were in agreement in 98.8% of cases; only two cases were discordant (+/- and -/+). Globally, the two markers had the same sensitivity (90%), specificity (98%), negative (93.2%) and positive (96.9%) predictive values. For anti-gliadin antibodies, the IgG were more sensitive (88.6%) and the IgA more specific (93.9%).

CONCLUSION

IgA anti-tissue transglutaminase can be used instead of IgA anti-endomysium as a serological marker of screening and diagnosis of coeliac disease in children after 3 years.

Authors+Show Affiliations

Laboratoire d'immunologie, CHU Habib Bourguiba, Sfax, Tunisie.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article

Language

fre

PubMed ID

15297237

Citation

Laadhar, L, et al. "[Determination of Anti-transglutaminase Antibodies in the Diagnosis of Coeliac Disease in Children: Results of a Five Year Prospective Study]." Annales De Biologie Clinique, vol. 62, no. 4, 2004, pp. 431-6.
Laadhar L, Bouaziz N, Ben Ayed M, et al. [Determination of anti-transglutaminase antibodies in the diagnosis of coeliac disease in children: results of a five year prospective study]. Ann Biol Clin (Paris). 2004;62(4):431-6.
Laadhar, L., Bouaziz, N., Ben Ayed, M., Chaabouni, M., Boudawara, T., Hachicha, M., ... Masmoudi, H. (2004). [Determination of anti-transglutaminase antibodies in the diagnosis of coeliac disease in children: results of a five year prospective study]. Annales De Biologie Clinique, 62(4), pp. 431-6.
Laadhar L, et al. [Determination of Anti-transglutaminase Antibodies in the Diagnosis of Coeliac Disease in Children: Results of a Five Year Prospective Study]. Ann Biol Clin (Paris). 2004;62(4):431-6. PubMed PMID: 15297237.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Determination of anti-transglutaminase antibodies in the diagnosis of coeliac disease in children: results of a five year prospective study]. AU - Laadhar,L, AU - Bouaziz,N, AU - Ben Ayed,M, AU - Chaabouni,M, AU - Boudawara,T, AU - Hachicha,M, AU - Jliri,R, AU - Triki,A, AU - Masmoudi,H, PY - 2004/8/7/pubmed PY - 2004/9/24/medline PY - 2004/8/7/entrez SP - 431 EP - 6 JF - Annales de biologie clinique JO - Ann. Biol. Clin. (Paris) VL - 62 IS - 4 N2 - AIM: To evaluate the interest of IgA antibodies to tissue transglutaminase in the diagnosis of children coeliac disease compared with anti-endomysium and anti-gliadin antibodies. SUBJECTS AND METHODS: Seventy children with coeliac disease (mean age: 5 years and 8 months) and 99 disease controls (mean age: 4 years and 5 months). IgA anti-transglutaminase were tested by ELISA using a human recombinant tissue transglutaminase. IgA anti-endomysium were detected by indirect immunofluorescence on monkey oesophagus. RESULTS: The middle rate of IgA anti-transglutaminase was 101.06 units in patients and only 0.47 unit in controls. IgA anti-transglutaminase and IgA anti-endomysium were in agreement in 98.8% of cases; only two cases were discordant (+/- and -/+). Globally, the two markers had the same sensitivity (90%), specificity (98%), negative (93.2%) and positive (96.9%) predictive values. For anti-gliadin antibodies, the IgG were more sensitive (88.6%) and the IgA more specific (93.9%). CONCLUSION: IgA anti-tissue transglutaminase can be used instead of IgA anti-endomysium as a serological marker of screening and diagnosis of coeliac disease in children after 3 years. SN - 0003-3898 UR - https://www.unboundmedicine.com/medline/citation/15297237/[Determination_of_anti_transglutaminase_antibodies_in_the_diagnosis_of_coeliac_disease_in_children:_results_of_a_five_year_prospective_study]_ L2 - http://www.jle.com/medline.md?issn=0003-3898&vol=62&iss=4&page=431 DB - PRIME DP - Unbound Medicine ER -