Crystallization and preliminary X-ray crystallographic analysis of chitosanase from Bacillus circulans MH-K1.Acta Crystallogr D Biol Crystallogr. 1995 Sep 01; 51(Pt 5):856-7.AC
Abstract
Chitosanase, an enzyme which hydrolyzes chitosan, isolated from Bacillus circulans MH-K1, was crystallized by a vapor-diffusion procedure at 293 K using ammonium sulfate as a precipitant. Rod-shaped colorless crystals, which grew to 0.05 x 0.15 x 1.2 mm within a week, belong to the orthorhombic system and the space group P222(1) or P2(1)2(1)2 with unit-cell dimensions of a = 43.3, b = 57.7, and c = 128.0 A. The asymmetric unit is thought to contain one chitosanase molecule (29 024 Da). The crystals diffract X-rays to at least 2.3 A resolution and are suitable for high-resolution X-ray structure analysis.
Pub Type(s)
Journal Article
Language
eng
PubMed ID
15299827
Citation
Saito, J I., et al. "Crystallization and Preliminary X-ray Crystallographic Analysis of Chitosanase From Bacillus Circulans MH-K1." Acta Crystallographica. Section D, Biological Crystallography, vol. 51, no. Pt 5, 1995, pp. 856-7.
Saito JI, Nagata Y, Ando A, et al. Crystallization and preliminary X-ray crystallographic analysis of chitosanase from Bacillus circulans MH-K1. Acta Crystallogr D Biol Crystallogr. 1995;51(Pt 5):856-7.
Saito, J. I., Nagata, Y., Ando, A., Kita, A., & Miki, K. (1995). Crystallization and preliminary X-ray crystallographic analysis of chitosanase from Bacillus circulans MH-K1. Acta Crystallographica. Section D, Biological Crystallography, 51(Pt 5), 856-7.
Saito JI, et al. Crystallization and Preliminary X-ray Crystallographic Analysis of Chitosanase From Bacillus Circulans MH-K1. Acta Crystallogr D Biol Crystallogr. 1995 Sep 1;51(Pt 5):856-7. PubMed PMID: 15299827.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR
T1 - Crystallization and preliminary X-ray crystallographic analysis of chitosanase from Bacillus circulans MH-K1.
AU - Saito,J I,
AU - Nagata,Y,
AU - Ando,A,
AU - Kita,A,
AU - Miki,K,
PY - 1995/9/1/pubmed
PY - 1995/9/1/medline
PY - 1995/9/1/entrez
SP - 856
EP - 7
JF - Acta crystallographica. Section D, Biological crystallography
JO - Acta Crystallogr D Biol Crystallogr
VL - 51
IS - Pt 5
N2 - Chitosanase, an enzyme which hydrolyzes chitosan, isolated from Bacillus circulans MH-K1, was crystallized by a vapor-diffusion procedure at 293 K using ammonium sulfate as a precipitant. Rod-shaped colorless crystals, which grew to 0.05 x 0.15 x 1.2 mm within a week, belong to the orthorhombic system and the space group P222(1) or P2(1)2(1)2 with unit-cell dimensions of a = 43.3, b = 57.7, and c = 128.0 A. The asymmetric unit is thought to contain one chitosanase molecule (29 024 Da). The crystals diffract X-rays to at least 2.3 A resolution and are suitable for high-resolution X-ray structure analysis.
SN - 0907-4449
UR - https://www.unboundmedicine.com/medline/citation/15299827/Crystallization_and_preliminary_X_ray_crystallographic_analysis_of_chitosanase_from_Bacillus_circulans_MH_K1_
DB - PRIME
DP - Unbound Medicine
ER -
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