Crystallization and preliminary X-ray analysis of a novel plant lectin from Calystegia sepium.Acta Crystallogr D Biol Crystallogr. 1997 Mar 01; 53(Pt 2):220-1.AC
Abstract
A mannose-specific lectin from Calystegia sepium has been crystallized by the hanging-drop vapour-diffusion method using ammonium sulfate as precipitant. The needle-shaped crystals are orthorhombic, space group C222(1) with cell dimensions a = 55.2(1), b = 55.9 (1), c = 196.1 (1) A. Fresh crystals diffract to 1.9 A resolution on a synchrotron radiation source. The asymmetric unit contains a dimer of two identical 16 kDa subunits with a packing density of 2.36 A(3) Da(-1). Intensity data have been observed beyond 2.0 A, but reasonable statistics restricted the usable range to 2.0 A.
Pub Type(s)
Journal Article
Language
eng
PubMed ID
15299962
Citation
Wright, L M., et al. "Crystallization and Preliminary X-ray Analysis of a Novel Plant Lectin From Calystegia Sepium." Acta Crystallographica. Section D, Biological Crystallography, vol. 53, no. Pt 2, 1997, pp. 220-1.
Wright LM, Wood SD, Reynolds CD, et al. Crystallization and preliminary X-ray analysis of a novel plant lectin from Calystegia sepium. Acta Crystallogr D Biol Crystallogr. 1997;53(Pt 2):220-1.
Wright, L. M., Wood, S. D., Reynolds, C. D., Rizkallah, P. J., Van Damme, E. J., & Peumans, W. J. (1997). Crystallization and preliminary X-ray analysis of a novel plant lectin from Calystegia sepium. Acta Crystallographica. Section D, Biological Crystallography, 53(Pt 2), 220-1.
Wright LM, et al. Crystallization and Preliminary X-ray Analysis of a Novel Plant Lectin From Calystegia Sepium. Acta Crystallogr D Biol Crystallogr. 1997 Mar 1;53(Pt 2):220-1. PubMed PMID: 15299962.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR
T1 - Crystallization and preliminary X-ray analysis of a novel plant lectin from Calystegia sepium.
AU - Wright,L M,
AU - Wood,S D,
AU - Reynolds,C D,
AU - Rizkallah,P J,
AU - Van Damme,E J,
AU - Peumans,W J,
PY - 1997/3/1/pubmed
PY - 1997/3/1/medline
PY - 1997/3/1/entrez
SP - 220
EP - 1
JF - Acta crystallographica. Section D, Biological crystallography
JO - Acta Crystallogr D Biol Crystallogr
VL - 53
IS - Pt 2
N2 - A mannose-specific lectin from Calystegia sepium has been crystallized by the hanging-drop vapour-diffusion method using ammonium sulfate as precipitant. The needle-shaped crystals are orthorhombic, space group C222(1) with cell dimensions a = 55.2(1), b = 55.9 (1), c = 196.1 (1) A. Fresh crystals diffract to 1.9 A resolution on a synchrotron radiation source. The asymmetric unit contains a dimer of two identical 16 kDa subunits with a packing density of 2.36 A(3) Da(-1). Intensity data have been observed beyond 2.0 A, but reasonable statistics restricted the usable range to 2.0 A.
SN - 0907-4449
UR - https://www.unboundmedicine.com/medline/citation/15299962/Crystallization_and_preliminary_X_ray_analysis_of_a_novel_plant_lectin_from_Calystegia_sepium_
DB - PRIME
DP - Unbound Medicine
ER -
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