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Mapping of 99 new microsatellite-derived loci in rye (Secale cereale L.) including 39 expressed sequence tags.
Theor Appl Genet. 2004 Aug; 109(4):725-32.TA

Abstract

The genetic map of rye contains predominantly restriction fragment length polymorphism (RFLP) markers but also a limited number of microsatellite markers, which are known to be more reliable and easier to apply. We report here the saturation of the genomic map of rye with additional microsatellite-derived markers that we obtained from the rye expressed sequence tag (EST) databases and the Gatersleben collection of wheat microsatellite markers (WMS). A total of 99 loci (39 EST and 60 WMS) were mapped into the RFLP frameworks of four rye mapping populations consisting of 139, 64, 58 and 60 RFLPs, respectively. For another ten WMS loci, which amplified PCR products not polymorphic in any of the mapping populations, chromosome and chromosome arm locations were determined using wheat-rye addition lines. Chromosomes 1R, 2R, 3R, 4R, 5R, 6R and 7R were enriched with 9, 19, 9, 13, 27, 16 and 16 microsatellite loci, respectively. The microsatellite loci mapped were evenly distributed along the chromosomes, which is important for the further application of these markers for gene mapping or diversity studies in rye. Forty-four of the WMS loci mapped in rye were found to be homologous to those mapped in bread wheat (Triticum aestivum L.).

Authors+Show Affiliations

Khlestkina EK
Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstrasse 3, 06466 Gatersleben, Germany.
Than MH
No affiliation info available
Pestsova EG
No affiliation info available
Röder MS
No affiliation info available
Malyshev SV
No affiliation info available
Korzun V
No affiliation info available
Börner A
No affiliation info available

MeSH

Chromosome MappingExpressed Sequence TagsMicrosatellite RepeatsPolymorphism, Restriction Fragment LengthSecale

Pub Type(s)

Comparative Study
Journal Article

Language

eng

PubMed ID

15300380

Citation

Khlestkina, Elena K., et al. "Mapping of 99 New Microsatellite-derived Loci in Rye (Secale Cereale L.) Including 39 Expressed Sequence Tags." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 109, no. 4, 2004, pp. 725-32.
Khlestkina EK, Than MH, Pestsova EG, et al. Mapping of 99 new microsatellite-derived loci in rye (Secale cereale L.) including 39 expressed sequence tags. Theor Appl Genet. 2004;109(4):725-32.
Khlestkina, E. K., Than, M. H., Pestsova, E. G., Röder, M. S., Malyshev, S. V., Korzun, V., & Börner, A. (2004). Mapping of 99 new microsatellite-derived loci in rye (Secale cereale L.) including 39 expressed sequence tags. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 109(4), 725-32.
Khlestkina EK, et al. Mapping of 99 New Microsatellite-derived Loci in Rye (Secale Cereale L.) Including 39 Expressed Sequence Tags. Theor Appl Genet. 2004;109(4):725-32. PubMed PMID: 15300380.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Mapping of 99 new microsatellite-derived loci in rye (Secale cereale L.) including 39 expressed sequence tags. AU - Khlestkina,Elena K, AU - Than,Ma Hla Myint, AU - Pestsova,Elena G, AU - Röder,Marion S, AU - Malyshev,Sergey V, AU - Korzun,Viktor, AU - Börner,Andreas, Y1 - 2004/08/06/ PY - 2003/09/16/received PY - 2004/03/08/accepted PY - 2004/8/10/pubmed PY - 2004/12/22/medline PY - 2004/8/10/entrez SP - 725 EP - 32 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 109 IS - 4 N2 - The genetic map of rye contains predominantly restriction fragment length polymorphism (RFLP) markers but also a limited number of microsatellite markers, which are known to be more reliable and easier to apply. We report here the saturation of the genomic map of rye with additional microsatellite-derived markers that we obtained from the rye expressed sequence tag (EST) databases and the Gatersleben collection of wheat microsatellite markers (WMS). A total of 99 loci (39 EST and 60 WMS) were mapped into the RFLP frameworks of four rye mapping populations consisting of 139, 64, 58 and 60 RFLPs, respectively. For another ten WMS loci, which amplified PCR products not polymorphic in any of the mapping populations, chromosome and chromosome arm locations were determined using wheat-rye addition lines. Chromosomes 1R, 2R, 3R, 4R, 5R, 6R and 7R were enriched with 9, 19, 9, 13, 27, 16 and 16 microsatellite loci, respectively. The microsatellite loci mapped were evenly distributed along the chromosomes, which is important for the further application of these markers for gene mapping or diversity studies in rye. Forty-four of the WMS loci mapped in rye were found to be homologous to those mapped in bread wheat (Triticum aestivum L.). SN - 0040-5752 UR - https://www.unboundmedicine.com/medline/citation/15300380/Mapping_of_99_new_microsatellite_derived_loci_in_rye__Secale_cereale_L___including_39_expressed_sequence_tags_ DB - PRIME DP - Unbound Medicine ER -