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Protein and sequence requirements for the recruitment of the human origin recognition complex to the latent cycle origin of DNA replication of Epstein-Barr virus oriP.
Virology. 2004 Sep 01; 326(2):317-28.V

Abstract

Initiation of DNA replication from within the Epstein-Barr virus (EBV) latent cycle origin oriP occurs once per cell cycle and is almost entirely dependent upon cellular proteins. The human origin recognition complex (ORC) is recruited to oriP and orchestrates the events that lead to the initiation of replication. EBNA-1, the sole viral protein required for oriP-plasmid replication, binds four sites within the replicator but the role(s) it plays in the replication of oriP plasmids has not been elucidated. We investigated the recruitment of ORC to oriP in vivo and show that the binding of EBNA-1 to the replicator is necessary for the association of the ORC subunit Orc2 with the replicator. The minimal replicator of oriP consists of two EBNA-1 binding sites flanked by perfect 14-bp inverted repeats (a and b), but these repeats are dispensable for the association of Orc2 with the replicator. A mutational analysis of the 14-bp repeats provided additional support for a role for the telomere repeat binding protein 2 in oriP replicator function. We show that nucleotide differences between the oriP replicator of the B95-8 and Raji EBV genomes are not solely responsible for the inefficient utilization of this origin in the Raji EBV genome.

Authors+Show Affiliations

Department of Molecular Genetics and Microbiology, Stony Brook University, NY 11794-5222, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15302216

Citation

Julien, Marcell Dodard, et al. "Protein and Sequence Requirements for the Recruitment of the Human Origin Recognition Complex to the Latent Cycle Origin of DNA Replication of Epstein-Barr Virus OriP." Virology, vol. 326, no. 2, 2004, pp. 317-28.
Julien MD, Polonskaya Z, Hearing J. Protein and sequence requirements for the recruitment of the human origin recognition complex to the latent cycle origin of DNA replication of Epstein-Barr virus oriP. Virology. 2004;326(2):317-28.
Julien, M. D., Polonskaya, Z., & Hearing, J. (2004). Protein and sequence requirements for the recruitment of the human origin recognition complex to the latent cycle origin of DNA replication of Epstein-Barr virus oriP. Virology, 326(2), 317-28.
Julien MD, Polonskaya Z, Hearing J. Protein and Sequence Requirements for the Recruitment of the Human Origin Recognition Complex to the Latent Cycle Origin of DNA Replication of Epstein-Barr Virus OriP. Virology. 2004 Sep 1;326(2):317-28. PubMed PMID: 15302216.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protein and sequence requirements for the recruitment of the human origin recognition complex to the latent cycle origin of DNA replication of Epstein-Barr virus oriP. AU - Julien,Marcell Dodard, AU - Polonskaya,Zhanna, AU - Hearing,Janet, PY - 2004/03/26/received PY - 2004/05/28/accepted PY - 2004/8/11/pubmed PY - 2004/10/2/medline PY - 2004/8/11/entrez SP - 317 EP - 28 JF - Virology JO - Virology VL - 326 IS - 2 N2 - Initiation of DNA replication from within the Epstein-Barr virus (EBV) latent cycle origin oriP occurs once per cell cycle and is almost entirely dependent upon cellular proteins. The human origin recognition complex (ORC) is recruited to oriP and orchestrates the events that lead to the initiation of replication. EBNA-1, the sole viral protein required for oriP-plasmid replication, binds four sites within the replicator but the role(s) it plays in the replication of oriP plasmids has not been elucidated. We investigated the recruitment of ORC to oriP in vivo and show that the binding of EBNA-1 to the replicator is necessary for the association of the ORC subunit Orc2 with the replicator. The minimal replicator of oriP consists of two EBNA-1 binding sites flanked by perfect 14-bp inverted repeats (a and b), but these repeats are dispensable for the association of Orc2 with the replicator. A mutational analysis of the 14-bp repeats provided additional support for a role for the telomere repeat binding protein 2 in oriP replicator function. We show that nucleotide differences between the oriP replicator of the B95-8 and Raji EBV genomes are not solely responsible for the inefficient utilization of this origin in the Raji EBV genome. SN - 0042-6822 UR - https://www.unboundmedicine.com/medline/citation/15302216/Protein_and_sequence_requirements_for_the_recruitment_of_the_human_origin_recognition_complex_to_the_latent_cycle_origin_of_DNA_replication_of_Epstein_Barr_virus_oriP_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0042682204004015 DB - PRIME DP - Unbound Medicine ER -