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Quantum dot biolabeling coupled with immunomagnetic separation for detection of Escherichia coli O157:H7.
Anal Chem. 2004 Aug 15; 76(16):4806-10.AC

Abstract

A sensitive, specific, and rapid method for the detection of E. coli O157:H7 was demonstrated using quantum dots (QDs) as a fluorescence marker coupled with immunomagnetic separation. Magnetic beads coated with anti-E. coli O157 antibodies were employed to selectively capture the target bacteria, and biotin-conjugated anti-E. coli antibodies were added to form sandwich immuno complexes. After magnetic separation, the immuno complexes were labeled with QDs via biotin-streptavidin conjugation. This was followed by a fluorescence measurement using a laptop-controlled portable device, which consisted of a blue LED and a CCD-array spectrometer. The peak intensity of the fluorescence emission was proportional to the initial cell concentration of E. coli O157:H7 in the range of 10(3)-10(7) CFU/mL with a detection limit at least 100 times lower than that of the FITC-based method. The total detection time was less than 2 h. Neither E. coli K12 nor Salmonella typhimurium interfered with the detection of E. coli O157:H7.

Authors+Show Affiliations

Su XL
Department of Biological and Agricultural Engineering, University of Arkansas, Fayetteville, Arkansas 72701, USA.
Li Y
No affiliation info available

MeSH

Escherichia coli O157Immunomagnetic SeparationIndicators and ReagentsQuantum TheorySafetySalmonella typhimuriumSpectrometry, Fluorescence

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

15307792

Citation

Su, Xiao-Li, and Yanbin Li. "Quantum Dot Biolabeling Coupled With Immunomagnetic Separation for Detection of Escherichia Coli O157:H7." Analytical Chemistry, vol. 76, no. 16, 2004, pp. 4806-10.
Su XL, Li Y. Quantum dot biolabeling coupled with immunomagnetic separation for detection of Escherichia coli O157:H7. Anal Chem. 2004;76(16):4806-10.
Su, X. L., & Li, Y. (2004). Quantum dot biolabeling coupled with immunomagnetic separation for detection of Escherichia coli O157:H7. Analytical Chemistry, 76(16), 4806-10.
Su XL, Li Y. Quantum Dot Biolabeling Coupled With Immunomagnetic Separation for Detection of Escherichia Coli O157:H7. Anal Chem. 2004 Aug 15;76(16):4806-10. PubMed PMID: 15307792.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Quantum dot biolabeling coupled with immunomagnetic separation for detection of Escherichia coli O157:H7. AU - Su,Xiao-Li, AU - Li,Yanbin, PY - 2004/8/17/pubmed PY - 2006/5/3/medline PY - 2004/8/17/entrez SP - 4806 EP - 10 JF - Analytical chemistry JO - Anal Chem VL - 76 IS - 16 N2 - A sensitive, specific, and rapid method for the detection of E. coli O157:H7 was demonstrated using quantum dots (QDs) as a fluorescence marker coupled with immunomagnetic separation. Magnetic beads coated with anti-E. coli O157 antibodies were employed to selectively capture the target bacteria, and biotin-conjugated anti-E. coli antibodies were added to form sandwich immuno complexes. After magnetic separation, the immuno complexes were labeled with QDs via biotin-streptavidin conjugation. This was followed by a fluorescence measurement using a laptop-controlled portable device, which consisted of a blue LED and a CCD-array spectrometer. The peak intensity of the fluorescence emission was proportional to the initial cell concentration of E. coli O157:H7 in the range of 10(3)-10(7) CFU/mL with a detection limit at least 100 times lower than that of the FITC-based method. The total detection time was less than 2 h. Neither E. coli K12 nor Salmonella typhimurium interfered with the detection of E. coli O157:H7. SN - 0003-2700 UR - https://www.unboundmedicine.com/medline/citation/15307792/Quantum_dot_biolabeling_coupled_with_immunomagnetic_separation_for_detection_of_Escherichia_coli_O157:H7_ DB - PRIME DP - Unbound Medicine ER -