Tags

Type your tag names separated by a space and hit enter

Enterocyte actin autoantibody detection: a new diagnostic tool in celiac disease diagnosis: results of a multicenter study.
Am J Gastroenterol. 2004 Aug; 99(8):1551-6.AJ

Abstract

OBJECTIVES

This study describes a new method to detect autoantibodies against actin filaments (AAA) as a serological marker of intestinal villous atrophy (IVA) in celiac disease (CD), and reports the results of an Italian double-blind multicenter study.

METHODS

IgA-AAA were analyzed by immunofluorescence using a newly developed method based on intestinal epithelial cells cultured in presence of colchicine. IgA-AAA were blindly evaluated prospectively in 223 antiendomysial antibody (AEA) and/or antitransglutaminase antibody (TGA) positive subjects and in 78 AEA and TGA negative subjects. IgA-AAA positive patients underwent an intestinal biopsy to confirm the diagnosis. Moreover, IgA-AAA were retrospectively investigated in 84 biopsy-proven CD patients and in 2,000 new consecutively collected serum samples from AEA and TGA negative nonbiopsied subjects.

RESULTS

IgA-AAA were positive in 98.2% of the CD patients with flat mucosa, in 89% with subtotal villous atrophy, and in 30% with partial villous atrophy. IgA-AAA were present in none of the AEA and TGA negative nonbiopsied controls. In AEA and/or TGA positive CD patients IgA-AAA positivity significantly correlated with IVA (p < 0.000 in the prospective study, p = 0.005 in the retrospective study). In the prospective study, the values of sensitivity, specificity, the positive predictive value, the negative predictive value, and the efficiency of the IgA-AAA test to identify patients with IVA were, respectively, 83.9%, 95.1%, 97.8%, 69.2%, and 87.0%. Furthermore, a significant correlation (p < 0.0001) was found between the IgA-AAA serum titre and the degree of IVA (rs 0.56).

CONCLUSIONS

The results of this multicenter study show that the new method for IgA-AAA detection could represent a practical diagnostic tool in AEA and/or TGA positive subjects, which would be especially useful when IVA shows a patchy distribution, when the histological picture is difficult to interpret, or when a biopsy could represent a life-threatening risk.

Authors+Show Affiliations

Department of Biomedical Sciences and Biotechnologies, Second Pediatrics Clinic, Cagliari University, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Multicenter Study
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15307876

Citation

Clemente, M G., et al. "Enterocyte Actin Autoantibody Detection: a New Diagnostic Tool in Celiac Disease Diagnosis: Results of a Multicenter Study." The American Journal of Gastroenterology, vol. 99, no. 8, 2004, pp. 1551-6.
Clemente MG, Musu MP, Troncone R, et al. Enterocyte actin autoantibody detection: a new diagnostic tool in celiac disease diagnosis: results of a multicenter study. Am J Gastroenterol. 2004;99(8):1551-6.
Clemente, M. G., Musu, M. P., Troncone, R., Volta, U., Congia, M., Ciacci, C., Neri, E., Not, T., Maggiore, G., Strisciuglio, P., Corazza, G. R., Gasbarrini, G., Cicotto, L., Sole, G., Fasano, A., & De Virgiliis, S. (2004). Enterocyte actin autoantibody detection: a new diagnostic tool in celiac disease diagnosis: results of a multicenter study. The American Journal of Gastroenterology, 99(8), 1551-6.
Clemente MG, et al. Enterocyte Actin Autoantibody Detection: a New Diagnostic Tool in Celiac Disease Diagnosis: Results of a Multicenter Study. Am J Gastroenterol. 2004;99(8):1551-6. PubMed PMID: 15307876.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enterocyte actin autoantibody detection: a new diagnostic tool in celiac disease diagnosis: results of a multicenter study. AU - Clemente,M G, AU - Musu,M P, AU - Troncone,R, AU - Volta,U, AU - Congia,M, AU - Ciacci,C, AU - Neri,E, AU - Not,T, AU - Maggiore,G, AU - Strisciuglio,P, AU - Corazza,G R, AU - Gasbarrini,G, AU - Cicotto,L, AU - Sole,G, AU - Fasano,A, AU - De Virgiliis,S, PY - 2004/8/17/pubmed PY - 2004/9/21/medline PY - 2004/8/17/entrez SP - 1551 EP - 6 JF - The American journal of gastroenterology JO - Am. J. Gastroenterol. VL - 99 IS - 8 N2 - OBJECTIVES: This study describes a new method to detect autoantibodies against actin filaments (AAA) as a serological marker of intestinal villous atrophy (IVA) in celiac disease (CD), and reports the results of an Italian double-blind multicenter study. METHODS: IgA-AAA were analyzed by immunofluorescence using a newly developed method based on intestinal epithelial cells cultured in presence of colchicine. IgA-AAA were blindly evaluated prospectively in 223 antiendomysial antibody (AEA) and/or antitransglutaminase antibody (TGA) positive subjects and in 78 AEA and TGA negative subjects. IgA-AAA positive patients underwent an intestinal biopsy to confirm the diagnosis. Moreover, IgA-AAA were retrospectively investigated in 84 biopsy-proven CD patients and in 2,000 new consecutively collected serum samples from AEA and TGA negative nonbiopsied subjects. RESULTS: IgA-AAA were positive in 98.2% of the CD patients with flat mucosa, in 89% with subtotal villous atrophy, and in 30% with partial villous atrophy. IgA-AAA were present in none of the AEA and TGA negative nonbiopsied controls. In AEA and/or TGA positive CD patients IgA-AAA positivity significantly correlated with IVA (p < 0.000 in the prospective study, p = 0.005 in the retrospective study). In the prospective study, the values of sensitivity, specificity, the positive predictive value, the negative predictive value, and the efficiency of the IgA-AAA test to identify patients with IVA were, respectively, 83.9%, 95.1%, 97.8%, 69.2%, and 87.0%. Furthermore, a significant correlation (p < 0.0001) was found between the IgA-AAA serum titre and the degree of IVA (rs 0.56). CONCLUSIONS: The results of this multicenter study show that the new method for IgA-AAA detection could represent a practical diagnostic tool in AEA and/or TGA positive subjects, which would be especially useful when IVA shows a patchy distribution, when the histological picture is difficult to interpret, or when a biopsy could represent a life-threatening risk. SN - 0002-9270 UR - https://www.unboundmedicine.com/medline/citation/15307876/Enterocyte_actin_autoantibody_detection:_a_new_diagnostic_tool_in_celiac_disease_diagnosis:_results_of_a_multicenter_study_ L2 - http://Insights.ovid.com/pubmed?pmid=15307876 DB - PRIME DP - Unbound Medicine ER -