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The F-box protein AhSLF-S2 controls the pollen function of S-RNase-based self-incompatibility.
Plant Cell. 2004 Sep; 16(9):2307-22.PC

Abstract

Recently, we have provided evidence that the polymorphic self-incompatibility (S) locus-encoded F-box (SLF) protein AhSLF-S(2) plays a role in mediating a selective S-RNase destruction during the self-incompatible response in Antirrhinum hispanicum. To investigate its role further, we first transformed a transformation-competent artificial chromosome clone (TAC26) containing both AhSLF-S(2) and AhS(2)-RNase into a self-incompatible (SI) line of Petunia hybrida. Molecular analyses showed that both genes are correctly expressed in pollen and pistil in four independent transgenic lines of petunia. Pollination tests indicated that all four lines became self-compatible because of the specific loss of the pollen function of SI. This alteration was transmitted stably into the T1 progeny. We then transformed AhSLF-S(2) cDNA under the control of a tomato (Lycopersicon esculentum) pollen-specific promoter LAT52 into the self-incompatible petunia line. Molecular studies revealed that AhSLF-S(2) is specifically expressed in pollen of five independent transgenic plants. Pollination tests showed that they also had lost the pollen function of SI. Importantly, expression of endogenous SLF or SLF-like genes was not altered in these transgenic plants. These results phenocopy a well-known phenomenon called competitive interaction whereby the presence of two different pollen S alleles within pollen leads to the breakdown of the pollen function of SI in several solanaceaous species. Furthermore, we demonstrated that AhSLF-S(2) physically interacts with PhS(3)-RNase from the P. hybrida line used for transformation. Together with the recent demonstration of PiSLF as the pollen determinant in P. inflata, these results provide direct evidence that the polymorphic SLF including AhSLF-S(2) controls the pollen function of S-RNase-based self-incompatibility.

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Authors+Show Affiliations

Qiao H
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100080, China.
Wang F
No affiliation info available
Zhao L
No affiliation info available
Zhou J
No affiliation info available
Lai Z
No affiliation info available
Zhang Y
No affiliation info available
Robbins TP
No affiliation info available
Xue Y
No affiliation info available

MeSH

Amino Acid SequenceAntirrhinumBase SequenceF-Box MotifsFlowersGene Expression Regulation, PlantSolanum lycopersicumMolecular Sequence DataPeptidesPetuniaPhenotypePhylogenyPlant ProteinsPlants, Genetically ModifiedPollenPromoter Regions, GeneticReproductionRibonucleasesSequence Homology, Amino AcidTransformation, Genetic

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15308757

Citation

Qiao, Hong, et al. "The F-box Protein AhSLF-S2 Controls the Pollen Function of S-RNase-based Self-incompatibility." The Plant Cell, vol. 16, no. 9, 2004, pp. 2307-22.
Qiao H, Wang F, Zhao L, et al. The F-box protein AhSLF-S2 controls the pollen function of S-RNase-based self-incompatibility. Plant Cell. 2004;16(9):2307-22.
Qiao, H., Wang, F., Zhao, L., Zhou, J., Lai, Z., Zhang, Y., Robbins, T. P., & Xue, Y. (2004). The F-box protein AhSLF-S2 controls the pollen function of S-RNase-based self-incompatibility. The Plant Cell, 16(9), 2307-22.
Qiao H, et al. The F-box Protein AhSLF-S2 Controls the Pollen Function of S-RNase-based Self-incompatibility. Plant Cell. 2004;16(9):2307-22. PubMed PMID: 15308757.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The F-box protein AhSLF-S2 controls the pollen function of S-RNase-based self-incompatibility. AU - Qiao,Hong, AU - Wang,Fei, AU - Zhao,Lan, AU - Zhou,Junli, AU - Lai,Zhao, AU - Zhang,Yansheng, AU - Robbins,Timothy P, AU - Xue,Yongbiao, Y1 - 2004/08/12/ PY - 2004/8/17/pubmed PY - 2004/12/16/medline PY - 2004/8/17/entrez SP - 2307 EP - 22 JF - The Plant cell JO - Plant Cell VL - 16 IS - 9 N2 - Recently, we have provided evidence that the polymorphic self-incompatibility (S) locus-encoded F-box (SLF) protein AhSLF-S(2) plays a role in mediating a selective S-RNase destruction during the self-incompatible response in Antirrhinum hispanicum. To investigate its role further, we first transformed a transformation-competent artificial chromosome clone (TAC26) containing both AhSLF-S(2) and AhS(2)-RNase into a self-incompatible (SI) line of Petunia hybrida. Molecular analyses showed that both genes are correctly expressed in pollen and pistil in four independent transgenic lines of petunia. Pollination tests indicated that all four lines became self-compatible because of the specific loss of the pollen function of SI. This alteration was transmitted stably into the T1 progeny. We then transformed AhSLF-S(2) cDNA under the control of a tomato (Lycopersicon esculentum) pollen-specific promoter LAT52 into the self-incompatible petunia line. Molecular studies revealed that AhSLF-S(2) is specifically expressed in pollen of five independent transgenic plants. Pollination tests showed that they also had lost the pollen function of SI. Importantly, expression of endogenous SLF or SLF-like genes was not altered in these transgenic plants. These results phenocopy a well-known phenomenon called competitive interaction whereby the presence of two different pollen S alleles within pollen leads to the breakdown of the pollen function of SI in several solanaceaous species. Furthermore, we demonstrated that AhSLF-S(2) physically interacts with PhS(3)-RNase from the P. hybrida line used for transformation. Together with the recent demonstration of PiSLF as the pollen determinant in P. inflata, these results provide direct evidence that the polymorphic SLF including AhSLF-S(2) controls the pollen function of S-RNase-based self-incompatibility. SN - 1040-4651 UR - https://www.unboundmedicine.com/medline/citation/15308757/The_F_box_protein_AhSLF_S2_controls_the_pollen_function_of_S_RNase_based_self_incompatibility_ DB - PRIME DP - Unbound Medicine ER -