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Distribution of PAI-1 promoter 4G/5G polymorphism in healthy Chinese and functional characterization using a luciferase reporter vector.
Ann Hematol. 2005 Mar; 84(3):183-7.AH

Abstract

The aim of this study was to analyze the distribution of plasminogen activator inhibitor-1 (PAI-1) promoter 4G/5G polymorphism in healthy Chinese and compare the transcription activity of the two PAI-1 promoters using luciferase reporter vector pGL(3). PAI-1 promoter 4G/5G polymorphism was analyzed in 77 healthy Chinese using allele-specific polymerase chain reaction (PCR). The PAI-1 promoters from individuals homozygous for the insertion (5G) and deletion (4G) were cloned into luciferase reporter vector pGL(3), respectively. The transcription activities of the two promoters (PAI-1-4G and PAI-1-5G) were then compared using the luciferase reporter vectors expressed in ECV304 and HepG(2) cell lines. Among the 77 healthy Chinese the genotype frequency for 4G/4G, 4G/5G, and 5G/5G was 49.1, 44.1, and 6.5%, respectively. Both of the two PAI-1 promoters cloned into luciferase reporter vector pGL(3), expressed very well both in ECV304 and HepG(2), and have similar basic transcription activity. Thrombin (10-20 U/ml) has no effects on the transcription activity of the two promoters expressed in ECV304, while in the HepG(2) cell line interleukin-1 (IL-1) 158 U/ml increased the transcription activity of PAI-1-4G by 67% (p<0.05, n=5) without effects on PAI-1-5G transcription activity. The distribution of PAI-1 promoter 4G/5G polymorphism in Chinese is obviously different from that in Caucasians with the 4G allele more frequently found in Chinese. PAI-1 promoter 4G/5G polymorphism does not alter basic transcription activity and 4G promoter has increased response to IL-1.

Authors+Show Affiliations

Ding Z
Department of Hematology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, 1 Shuaifuyuan, Beijing 100730, China. zding@temple.edu
Pan JQ
No affiliation info available

MeSH

Cell Line, TumorChinaGene FrequencyGenes, ReporterGenotypeHumansInterleukin-1LuciferasesPlasminogen Activator Inhibitor 1Polymorphism, GeneticPromoter Regions, GeneticThrombinTranscription, GeneticTransfection

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15309528

Citation

Ding, Zhongren, and Jia-Qi Pan. "Distribution of PAI-1 Promoter 4G/5G Polymorphism in Healthy Chinese and Functional Characterization Using a Luciferase Reporter Vector." Annals of Hematology, vol. 84, no. 3, 2005, pp. 183-7.
Ding Z, Pan JQ. Distribution of PAI-1 promoter 4G/5G polymorphism in healthy Chinese and functional characterization using a luciferase reporter vector. Ann Hematol. 2005;84(3):183-7.
Ding, Z., & Pan, J. Q. (2005). Distribution of PAI-1 promoter 4G/5G polymorphism in healthy Chinese and functional characterization using a luciferase reporter vector. Annals of Hematology, 84(3), 183-7.
Ding Z, Pan JQ. Distribution of PAI-1 Promoter 4G/5G Polymorphism in Healthy Chinese and Functional Characterization Using a Luciferase Reporter Vector. Ann Hematol. 2005;84(3):183-7. PubMed PMID: 15309528.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Distribution of PAI-1 promoter 4G/5G polymorphism in healthy Chinese and functional characterization using a luciferase reporter vector. AU - Ding,Zhongren, AU - Pan,Jia-Qi, Y1 - 2004/08/12/ PY - 2003/12/19/received PY - 2004/07/10/accepted PY - 2004/8/17/pubmed PY - 2005/5/18/medline PY - 2004/8/17/entrez SP - 183 EP - 7 JF - Annals of hematology JO - Ann Hematol VL - 84 IS - 3 N2 - The aim of this study was to analyze the distribution of plasminogen activator inhibitor-1 (PAI-1) promoter 4G/5G polymorphism in healthy Chinese and compare the transcription activity of the two PAI-1 promoters using luciferase reporter vector pGL(3). PAI-1 promoter 4G/5G polymorphism was analyzed in 77 healthy Chinese using allele-specific polymerase chain reaction (PCR). The PAI-1 promoters from individuals homozygous for the insertion (5G) and deletion (4G) were cloned into luciferase reporter vector pGL(3), respectively. The transcription activities of the two promoters (PAI-1-4G and PAI-1-5G) were then compared using the luciferase reporter vectors expressed in ECV304 and HepG(2) cell lines. Among the 77 healthy Chinese the genotype frequency for 4G/4G, 4G/5G, and 5G/5G was 49.1, 44.1, and 6.5%, respectively. Both of the two PAI-1 promoters cloned into luciferase reporter vector pGL(3), expressed very well both in ECV304 and HepG(2), and have similar basic transcription activity. Thrombin (10-20 U/ml) has no effects on the transcription activity of the two promoters expressed in ECV304, while in the HepG(2) cell line interleukin-1 (IL-1) 158 U/ml increased the transcription activity of PAI-1-4G by 67% (p<0.05, n=5) without effects on PAI-1-5G transcription activity. The distribution of PAI-1 promoter 4G/5G polymorphism in Chinese is obviously different from that in Caucasians with the 4G allele more frequently found in Chinese. PAI-1 promoter 4G/5G polymorphism does not alter basic transcription activity and 4G promoter has increased response to IL-1. SN - 0939-5555 UR - https://www.unboundmedicine.com/medline/citation/15309528/Distribution_of_PAI_1_promoter_4G/5G_polymorphism_in_healthy_Chinese_and_functional_characterization_using_a_luciferase_reporter_vector_ DB - PRIME DP - Unbound Medicine ER -