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Vascular endothelial growth factor-A is expressed both on lymphoma cells and endothelial cells in angioimmunoblastic T-cell lymphoma and related to lymphoma progression.
Lab Invest. 2004 Nov; 84(11):1512-9.LI

Abstract

Vascular endothelial growth factor-A (VEGF-A), a main stimulator of endothelial cell proliferation, plays an important role on tumor angiogenesis. Angioimmunoblastic T-cell lymphoma (AITL) show the most prominent vascular component among lymphomas and their prognosis is difficult to predict. To assess the clinical significance of VEGF-A in AITL, VEGF-A gene expression was studied in the tumoral lymph nodes of 24 patients using laser microdissection and quantitative polymerase chain reaction. VEGF-A gene was overexpressed in both microdissected lymphoma and endothelial cells. Increased levels of VEGF-A gene expression in lymphoma cells, as in endothelial cells, were related to extranodal involvement and to short survival time. Accordingly, VEGF-A protein expression was also found in both types of cells in lymph nodes and bone marrows with lymphomatous involvement. Triple immunofluorescent labeling on lymph node sections showed that VEGF-A protein and its receptor VEGF-R1 were coexpressed on endothelial cells of microvessels in the areas of lymphoma invasion. In these areas, ultrastructural study showed dystrophic microvessels. Taken together, the value of VEGF-A gene expression as an adverse prognostic marker in AITL should thus be considered. In addition to lymphoma cells themselves, the vascular component, a critical pathologic characteristic in AITL, also contributes to lymphoma progression.

Authors+Show Affiliations

ERM-0220, Institut Universitaire d'Hématologie, Université Paris VII, Paris, France.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15311211

Citation

Zhao, Wei-Li, et al. "Vascular Endothelial Growth factor-A Is Expressed Both On Lymphoma Cells and Endothelial Cells in Angioimmunoblastic T-cell Lymphoma and Related to Lymphoma Progression." Laboratory Investigation; a Journal of Technical Methods and Pathology, vol. 84, no. 11, 2004, pp. 1512-9.
Zhao WL, Mourah S, Mounier N, et al. Vascular endothelial growth factor-A is expressed both on lymphoma cells and endothelial cells in angioimmunoblastic T-cell lymphoma and related to lymphoma progression. Lab Invest. 2004;84(11):1512-9.
Zhao, W. L., Mourah, S., Mounier, N., Leboeuf, C., Daneshpouy, M. E., Legrès, L., Meignin, V., Oksenhendler, E., Maignin, C. L., Calvo, F., Brière, J., Gisselbrecht, C., & Janin, A. (2004). Vascular endothelial growth factor-A is expressed both on lymphoma cells and endothelial cells in angioimmunoblastic T-cell lymphoma and related to lymphoma progression. Laboratory Investigation; a Journal of Technical Methods and Pathology, 84(11), 1512-9.
Zhao WL, et al. Vascular Endothelial Growth factor-A Is Expressed Both On Lymphoma Cells and Endothelial Cells in Angioimmunoblastic T-cell Lymphoma and Related to Lymphoma Progression. Lab Invest. 2004;84(11):1512-9. PubMed PMID: 15311211.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Vascular endothelial growth factor-A is expressed both on lymphoma cells and endothelial cells in angioimmunoblastic T-cell lymphoma and related to lymphoma progression. AU - Zhao,Wei-Li, AU - Mourah,Samia, AU - Mounier,Nicolas, AU - Leboeuf,Christophe, AU - Daneshpouy,Marjan Ertault, AU - Legrès,Luc, AU - Meignin,Veronique, AU - Oksenhendler,Eric, AU - Maignin,Christine Le, AU - Calvo,Fabien, AU - Brière,Josette, AU - Gisselbrecht,Christian, AU - Janin,Anne, PY - 2004/8/18/pubmed PY - 2004/12/16/medline PY - 2004/8/18/entrez SP - 1512 EP - 9 JF - Laboratory investigation; a journal of technical methods and pathology JO - Lab Invest VL - 84 IS - 11 N2 - Vascular endothelial growth factor-A (VEGF-A), a main stimulator of endothelial cell proliferation, plays an important role on tumor angiogenesis. Angioimmunoblastic T-cell lymphoma (AITL) show the most prominent vascular component among lymphomas and their prognosis is difficult to predict. To assess the clinical significance of VEGF-A in AITL, VEGF-A gene expression was studied in the tumoral lymph nodes of 24 patients using laser microdissection and quantitative polymerase chain reaction. VEGF-A gene was overexpressed in both microdissected lymphoma and endothelial cells. Increased levels of VEGF-A gene expression in lymphoma cells, as in endothelial cells, were related to extranodal involvement and to short survival time. Accordingly, VEGF-A protein expression was also found in both types of cells in lymph nodes and bone marrows with lymphomatous involvement. Triple immunofluorescent labeling on lymph node sections showed that VEGF-A protein and its receptor VEGF-R1 were coexpressed on endothelial cells of microvessels in the areas of lymphoma invasion. In these areas, ultrastructural study showed dystrophic microvessels. Taken together, the value of VEGF-A gene expression as an adverse prognostic marker in AITL should thus be considered. In addition to lymphoma cells themselves, the vascular component, a critical pathologic characteristic in AITL, also contributes to lymphoma progression. SN - 0023-6837 UR - https://www.unboundmedicine.com/medline/citation/15311211/Vascular_endothelial_growth_factor_A_is_expressed_both_on_lymphoma_cells_and_endothelial_cells_in_angioimmunoblastic_T_cell_lymphoma_and_related_to_lymphoma_progression_ L2 - https://doi.org/10.1038/labinvest.3700145 DB - PRIME DP - Unbound Medicine ER -