Tags

Type your tag names separated by a space and hit enter

Novel aspects of accumulation dynamics and A beta composition in transgenic models of AD.
Neurobiol Aging. 2004 Oct; 25(9):1175-85.NA

Abstract

A homogeneous time-resolved fluorescence immunoassay for detection of beta-amyloid (A beta) peptides has been adapted for quantification of A beta(40) and A beta(42) accumulation in brains of APP695SWE transgenic mice. These over-express human beta APP(swe), beta-amyloid precursor protein (beta-APP) containing the K670N/M671L 'Swedish' familial Alzheimer's disease (FAD) mutation. Both peptides start to accumulate in this line from about 260 to 280 days of age. Co-expression of a human presenilin-1 (PS1) transgene containing the A246E FAD mutation accelerates deposition and also favors-at least initially-accumulation of A beta(42) so that the A beta(2):A beta(40) ratio of peptides from 7- to 12-month-old APP695SWE x PS1A246E animals is significantly elevated above that observed throughout the lifetime of APP695SWE mice. These findings, supported by parallel immunohistochemical staining and surface-enhanced laser desorption/ionization time-of-flight (SELDI-TOF) mass spectrometry data, offer important longitudinal characterization of two mouse models of cerebral amyloidosis. Application of the same extraction and quantitation procedures to samples of temporal cortex from AD sufferers indicates however that A beta(40) is only a minor component of beta-amyloid in humans.

Authors+Show Affiliations

Department of Molecular and Cellular Neuroscience, Merck Sharp & Dohme Research Laboratories, Neuroscience Research Centre, Terlings Park, Harlow, Essex CM20 2QR, UK.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15312963

Citation

Lewis, Huw D., et al. "Novel Aspects of Accumulation Dynamics and a Beta Composition in Transgenic Models of AD." Neurobiology of Aging, vol. 25, no. 9, 2004, pp. 1175-85.
Lewis HD, Beher D, Smith D, et al. Novel aspects of accumulation dynamics and A beta composition in transgenic models of AD. Neurobiol Aging. 2004;25(9):1175-85.
Lewis, H. D., Beher, D., Smith, D., Hewson, L., Cookson, N., Reynolds, D. S., Dawson, G. R., Jiang, M., Van der Ploeg, L. H., Qian, S., Rosahl, T. W., Kalaria, R. N., & Shearman, M. S. (2004). Novel aspects of accumulation dynamics and A beta composition in transgenic models of AD. Neurobiology of Aging, 25(9), 1175-85.
Lewis HD, et al. Novel Aspects of Accumulation Dynamics and a Beta Composition in Transgenic Models of AD. Neurobiol Aging. 2004;25(9):1175-85. PubMed PMID: 15312963.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Novel aspects of accumulation dynamics and A beta composition in transgenic models of AD. AU - Lewis,Huw D, AU - Beher,Dirk, AU - Smith,David, AU - Hewson,Louise, AU - Cookson,Natalie, AU - Reynolds,David S, AU - Dawson,Gerard R, AU - Jiang,Michael, AU - Van der Ploeg,Lex H T, AU - Qian,Su, AU - Rosahl,Thomas W, AU - Kalaria,Raj N, AU - Shearman,Mark S, PY - 2003/04/16/received PY - 2003/10/20/revised PY - 2003/12/11/accepted PY - 2004/8/18/pubmed PY - 2004/12/22/medline PY - 2004/8/18/entrez SP - 1175 EP - 85 JF - Neurobiology of aging JO - Neurobiol. Aging VL - 25 IS - 9 N2 - A homogeneous time-resolved fluorescence immunoassay for detection of beta-amyloid (A beta) peptides has been adapted for quantification of A beta(40) and A beta(42) accumulation in brains of APP695SWE transgenic mice. These over-express human beta APP(swe), beta-amyloid precursor protein (beta-APP) containing the K670N/M671L 'Swedish' familial Alzheimer's disease (FAD) mutation. Both peptides start to accumulate in this line from about 260 to 280 days of age. Co-expression of a human presenilin-1 (PS1) transgene containing the A246E FAD mutation accelerates deposition and also favors-at least initially-accumulation of A beta(42) so that the A beta(2):A beta(40) ratio of peptides from 7- to 12-month-old APP695SWE x PS1A246E animals is significantly elevated above that observed throughout the lifetime of APP695SWE mice. These findings, supported by parallel immunohistochemical staining and surface-enhanced laser desorption/ionization time-of-flight (SELDI-TOF) mass spectrometry data, offer important longitudinal characterization of two mouse models of cerebral amyloidosis. Application of the same extraction and quantitation procedures to samples of temporal cortex from AD sufferers indicates however that A beta(40) is only a minor component of beta-amyloid in humans. SN - 0197-4580 UR - https://www.unboundmedicine.com/medline/citation/15312963/Novel_aspects_of_accumulation_dynamics_and_A_beta_composition_in_transgenic_models_of_AD_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0197458004000478 DB - PRIME DP - Unbound Medicine ER -