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Expression and distribution of Na, K-ATPase in gill and kidney of the spotted green pufferfish, Tetraodon nigroviridis, in response to salinity challenge.
Comp Biochem Physiol A Mol Integr Physiol. 2004 Jul; 138(3):287-95.CB

Abstract

Freshwater (FW) spotted green pufferfish (Tetraodon nigroviridis) were transferred directly from a local aquarium to fresh water (FW; 0 per thousand), brackish water (BW; 15 per thousand), and seawater (SW; 35 per thousand) conditions in the laboratory and reared for at least two weeks. No mortality was found. To investigate the efficient mechanisms of osmoregulation in the euryhaline teleost, distribution and expression of Na,K-ATPase (NKA) in gill and kidney of the pufferfish were examined and the osmolality, [Na+] and [Cl-] of the blood were assayed. The lowest levels of both relative protein abundance and activity were found to be exhibited in the BW group, and higher levels in the SW group than FW group. In all salinities, branchial NKA immunoreactivity was found in epithelial cells of the interlamellar region of the filament and not on the lamellae. Relative abundance of kidney NKA alpha-subunit, as well as the NKA activity, was found to be higher in the FW pufferfish than fish in BW or SW. Renal NKA appeared in the epithelial cells of distal tubules, proximal tubules, and collecting tubules, but not in glomeruli, in fish groups of various salinities. Plasma osmolality and chloride levels were significantly lower in FW pufferfish than those in BW and SW, whereas plasma sodium did not differ among the groups. Although identical distributions of NKA were found in either gill or kidney of FW-, BW- or SW-acclimated spotted green pufferfish, differential NKA expression in fish of various salinity groups was associated with physiological homeostasis (stable blood osmolality), and illustrated the impressive osmoregulatory ability of this freshwater and estuarine species in response to salinity challenge.

Authors+Show Affiliations

Department of Life Sciences, National Chung-Hsing University, Taichung 402, Taiwan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15313482

Citation

Lin, C H., et al. "Expression and Distribution of Na, K-ATPase in Gill and Kidney of the Spotted Green Pufferfish, Tetraodon Nigroviridis, in Response to Salinity Challenge." Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, vol. 138, no. 3, 2004, pp. 287-95.
Lin CH, Tsai RS, Lee TH. Expression and distribution of Na, K-ATPase in gill and kidney of the spotted green pufferfish, Tetraodon nigroviridis, in response to salinity challenge. Comp Biochem Physiol A Mol Integr Physiol. 2004;138(3):287-95.
Lin, C. H., Tsai, R. S., & Lee, T. H. (2004). Expression and distribution of Na, K-ATPase in gill and kidney of the spotted green pufferfish, Tetraodon nigroviridis, in response to salinity challenge. Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, 138(3), 287-95.
Lin CH, Tsai RS, Lee TH. Expression and Distribution of Na, K-ATPase in Gill and Kidney of the Spotted Green Pufferfish, Tetraodon Nigroviridis, in Response to Salinity Challenge. Comp Biochem Physiol A Mol Integr Physiol. 2004;138(3):287-95. PubMed PMID: 15313482.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression and distribution of Na, K-ATPase in gill and kidney of the spotted green pufferfish, Tetraodon nigroviridis, in response to salinity challenge. AU - Lin,C H, AU - Tsai,R S, AU - Lee,T H, PY - 2003/10/24/received PY - 2004/02/14/revised PY - 2004/04/10/accepted PY - 2004/8/18/pubmed PY - 2005/7/23/medline PY - 2004/8/18/entrez SP - 287 EP - 95 JF - Comparative biochemistry and physiology. Part A, Molecular & integrative physiology JO - Comp Biochem Physiol A Mol Integr Physiol VL - 138 IS - 3 N2 - Freshwater (FW) spotted green pufferfish (Tetraodon nigroviridis) were transferred directly from a local aquarium to fresh water (FW; 0 per thousand), brackish water (BW; 15 per thousand), and seawater (SW; 35 per thousand) conditions in the laboratory and reared for at least two weeks. No mortality was found. To investigate the efficient mechanisms of osmoregulation in the euryhaline teleost, distribution and expression of Na,K-ATPase (NKA) in gill and kidney of the pufferfish were examined and the osmolality, [Na+] and [Cl-] of the blood were assayed. The lowest levels of both relative protein abundance and activity were found to be exhibited in the BW group, and higher levels in the SW group than FW group. In all salinities, branchial NKA immunoreactivity was found in epithelial cells of the interlamellar region of the filament and not on the lamellae. Relative abundance of kidney NKA alpha-subunit, as well as the NKA activity, was found to be higher in the FW pufferfish than fish in BW or SW. Renal NKA appeared in the epithelial cells of distal tubules, proximal tubules, and collecting tubules, but not in glomeruli, in fish groups of various salinities. Plasma osmolality and chloride levels were significantly lower in FW pufferfish than those in BW and SW, whereas plasma sodium did not differ among the groups. Although identical distributions of NKA were found in either gill or kidney of FW-, BW- or SW-acclimated spotted green pufferfish, differential NKA expression in fish of various salinity groups was associated with physiological homeostasis (stable blood osmolality), and illustrated the impressive osmoregulatory ability of this freshwater and estuarine species in response to salinity challenge. SN - 1095-6433 UR - https://www.unboundmedicine.com/medline/citation/15313482/Expression_and_distribution_of_Na_K_ATPase_in_gill_and_kidney_of_the_spotted_green_pufferfish_Tetraodon_nigroviridis_in_response_to_salinity_challenge_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1095643304001448 DB - PRIME DP - Unbound Medicine ER -