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Identification of functional mob regions in Rhizobium etli: evidence for self-transmissibility of the symbiotic plasmid pRetCFN42d.
J Bacteriol. 2004 Sep; 186(17):5753-61.JB

Abstract

An approach originally designed to identify functional origins of conjugative transfer (oriT or mob) in a bacterial genome (J. A. Herrera-Cervera, J. M. Sanjuán-Pinilla, J. Olivares, and J. Sanjuán, J. Bacteriol. 180:4583-4590, 1998) was modified to improve its reliability and prevent selection of undesired false mob clones. By following this modified approach, we were able to identify two functional mob regions in the genome of Rhizobium etli CFN42. One corresponds to the recently characterized transfer region of the nonsymbiotic, self-transmissible plasmid pRetCFN42a (C. Tun-Garrido, P. Bustos, V. González, and S. Brom, J. Bacteriol. 185:1681-1692, 2003), whereas the second mob region belongs to the symbiotic plasmid pRetCFN42d. The new transfer region identified contains a putative oriT and a typical conjugative (tra) gene cluster organization. Although pRetCFN42d had not previously been shown to be self-transmissible, mobilization of cosmids containing this tra region required the presence of a wild-type pRetCFN42d in the donor cell; the presence of multiple copies of this mob region in CFN42 also promoted conjugal transfer of the Sym plasmid pRetCFN42d. The overexpression of a small open reading frame, named yp028, located downstream of the putative relaxase gene traA, appeared to be responsible for promoting the conjugal transfer of the R. etli pSym under laboratory conditions. This yp028-dependent conjugal transfer required a wild-type pRetCFN42d traA gene. Our results suggest for the first time that the R. etli symbiotic plasmid is self-transmissible and that its transfer is subject to regulation. In wild-type CFN42, pRetCFN42d tra gene expression appears to be insufficient to promote plasmid transfer under standard laboratory conditions; gene yp028 may play some role in the activation of conjugal transfer in response to as-yet-unknown environmental conditions.

Authors+Show Affiliations

Departamento Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín. Profesor Albareda 1, 18008 Granada, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15317780

Citation

Pérez-Mendoza, Daniel, et al. "Identification of Functional Mob Regions in Rhizobium Etli: Evidence for Self-transmissibility of the Symbiotic Plasmid PRetCFN42d." Journal of Bacteriology, vol. 186, no. 17, 2004, pp. 5753-61.
Pérez-Mendoza D, Domínguez-Ferreras A, Muñoz S, et al. Identification of functional mob regions in Rhizobium etli: evidence for self-transmissibility of the symbiotic plasmid pRetCFN42d. J Bacteriol. 2004;186(17):5753-61.
Pérez-Mendoza, D., Domínguez-Ferreras, A., Muñoz, S., Soto, M. J., Olivares, J., Brom, S., Girard, L., Herrera-Cervera, J. A., & Sanjuán, J. (2004). Identification of functional mob regions in Rhizobium etli: evidence for self-transmissibility of the symbiotic plasmid pRetCFN42d. Journal of Bacteriology, 186(17), 5753-61.
Pérez-Mendoza D, et al. Identification of Functional Mob Regions in Rhizobium Etli: Evidence for Self-transmissibility of the Symbiotic Plasmid PRetCFN42d. J Bacteriol. 2004;186(17):5753-61. PubMed PMID: 15317780.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of functional mob regions in Rhizobium etli: evidence for self-transmissibility of the symbiotic plasmid pRetCFN42d. AU - Pérez-Mendoza,Daniel, AU - Domínguez-Ferreras,Ana, AU - Muñoz,Socorro, AU - Soto,María José, AU - Olivares,José, AU - Brom,Susana, AU - Girard,Lourdes, AU - Herrera-Cervera,José A, AU - Sanjuán,Juan, PY - 2004/8/20/pubmed PY - 2004/9/24/medline PY - 2004/8/20/entrez SP - 5753 EP - 61 JF - Journal of bacteriology JO - J. Bacteriol. VL - 186 IS - 17 N2 - An approach originally designed to identify functional origins of conjugative transfer (oriT or mob) in a bacterial genome (J. A. Herrera-Cervera, J. M. Sanjuán-Pinilla, J. Olivares, and J. Sanjuán, J. Bacteriol. 180:4583-4590, 1998) was modified to improve its reliability and prevent selection of undesired false mob clones. By following this modified approach, we were able to identify two functional mob regions in the genome of Rhizobium etli CFN42. One corresponds to the recently characterized transfer region of the nonsymbiotic, self-transmissible plasmid pRetCFN42a (C. Tun-Garrido, P. Bustos, V. González, and S. Brom, J. Bacteriol. 185:1681-1692, 2003), whereas the second mob region belongs to the symbiotic plasmid pRetCFN42d. The new transfer region identified contains a putative oriT and a typical conjugative (tra) gene cluster organization. Although pRetCFN42d had not previously been shown to be self-transmissible, mobilization of cosmids containing this tra region required the presence of a wild-type pRetCFN42d in the donor cell; the presence of multiple copies of this mob region in CFN42 also promoted conjugal transfer of the Sym plasmid pRetCFN42d. The overexpression of a small open reading frame, named yp028, located downstream of the putative relaxase gene traA, appeared to be responsible for promoting the conjugal transfer of the R. etli pSym under laboratory conditions. This yp028-dependent conjugal transfer required a wild-type pRetCFN42d traA gene. Our results suggest for the first time that the R. etli symbiotic plasmid is self-transmissible and that its transfer is subject to regulation. In wild-type CFN42, pRetCFN42d tra gene expression appears to be insufficient to promote plasmid transfer under standard laboratory conditions; gene yp028 may play some role in the activation of conjugal transfer in response to as-yet-unknown environmental conditions. SN - 0021-9193 UR - https://www.unboundmedicine.com/medline/citation/15317780/Identification_of_functional_mob_regions_in_Rhizobium_etli:_evidence_for_self_transmissibility_of_the_symbiotic_plasmid_pRetCFN42d_ L2 - http://jb.asm.org/cgi/pmidlookup?view=long&pmid=15317780 DB - PRIME DP - Unbound Medicine ER -