Tags

Type your tag names separated by a space and hit enter

Comparison of intracranial self-administration of ethanol within the posterior ventral tegmental area between alcohol-preferring and Wistar rats.
Alcohol Clin Exp Res. 2004 Aug; 28(8):1212-9.AC

Abstract

BACKGROUND

A previous study indicated that selectively bred alcohol-preferring (P) rats self-administered ethanol (EtOH) directly into the ventral tegmental area (VTA), whereas the alcohol-nonpreferring line did not. Wistar rats will also self-administer EtOH directly into the posterior VTA. Because Wistar rats also have a low preference for EtOH solutions but self-inject EtOH into the VTA, this study was undertaken to test the hypothesis that there is an association between EtOH preference and sensitivity of the VTA to the reinforcing effects of EtOH.

METHODS

Adult P and Wistar rats were assigned to groups that received one of the following concentrations of EtOH: 0, 50, 75, 100, 150, or 200 mg/100 ml. Rats were connected to the microinjection system, placed into two-lever (active and inactive) experimental chambers, and given EtOH for the first four sessions (acquisition), artificial cerebrospinal fluid for sessions 5 and 6 (extinction), and EtOH again in session 7 (reinstatement). Responding on the active lever produced a 100-nl injection of the infusate.

RESULTS

P rats self-infused 75 to 200 mg/100 ml EtOH and demonstrated lever discrimination, whereas Wistar rats reliably self-infused only 150 and 200 mg/100 ml EtOH. Both P and Wistar rats reduced responding on the active lever when artificial cerebrospinal fluid (aCSF) was substituted for EtOH and reinstated responding in session 7 when EtOH was restored, although P rats demonstrated a very robust enhancement of responding for 100 and 150 mg/100 ml EtOH, and this was not found for Wistar rats.

CONCLUSIONS

These results suggest that, compared with Wistar rats, the posterior VTA of P rats was more sensitive to the reinforcing effects of EtOH. Furthermore, the reinstatement data suggest that the posterior VTA of P rats underwent neuronal alterations as a result of prior EtOH exposure and extinction that changed the reinforcing effects of EtOH within this region.

Authors+Show Affiliations

Department of Psychiatry, Indiana University School of Medicine, Indianapolis, Indiana 46202-4887, USA. zrodd@iupui.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15318120

Citation

Rodd, Zachary A., et al. "Comparison of Intracranial Self-administration of Ethanol Within the Posterior Ventral Tegmental Area Between Alcohol-preferring and Wistar Rats." Alcoholism, Clinical and Experimental Research, vol. 28, no. 8, 2004, pp. 1212-9.
Rodd ZA, Bell RL, Melendez RI, et al. Comparison of intracranial self-administration of ethanol within the posterior ventral tegmental area between alcohol-preferring and Wistar rats. Alcohol Clin Exp Res. 2004;28(8):1212-9.
Rodd, Z. A., Bell, R. L., Melendez, R. I., Kuc, K. A., Lumeng, L., Li, T. K., Murphy, J. M., & McBride, W. J. (2004). Comparison of intracranial self-administration of ethanol within the posterior ventral tegmental area between alcohol-preferring and Wistar rats. Alcoholism, Clinical and Experimental Research, 28(8), 1212-9.
Rodd ZA, et al. Comparison of Intracranial Self-administration of Ethanol Within the Posterior Ventral Tegmental Area Between Alcohol-preferring and Wistar Rats. Alcohol Clin Exp Res. 2004;28(8):1212-9. PubMed PMID: 15318120.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of intracranial self-administration of ethanol within the posterior ventral tegmental area between alcohol-preferring and Wistar rats. AU - Rodd,Zachary A, AU - Bell,Richard L, AU - Melendez,Roberto I, AU - Kuc,Kelly A, AU - Lumeng,Lawrence, AU - Li,Ting-Kai, AU - Murphy,James M, AU - McBride,William J, PY - 2004/8/20/pubmed PY - 2005/2/5/medline PY - 2004/8/20/entrez SP - 1212 EP - 9 JF - Alcoholism, clinical and experimental research JO - Alcohol Clin Exp Res VL - 28 IS - 8 N2 - BACKGROUND: A previous study indicated that selectively bred alcohol-preferring (P) rats self-administered ethanol (EtOH) directly into the ventral tegmental area (VTA), whereas the alcohol-nonpreferring line did not. Wistar rats will also self-administer EtOH directly into the posterior VTA. Because Wistar rats also have a low preference for EtOH solutions but self-inject EtOH into the VTA, this study was undertaken to test the hypothesis that there is an association between EtOH preference and sensitivity of the VTA to the reinforcing effects of EtOH. METHODS: Adult P and Wistar rats were assigned to groups that received one of the following concentrations of EtOH: 0, 50, 75, 100, 150, or 200 mg/100 ml. Rats were connected to the microinjection system, placed into two-lever (active and inactive) experimental chambers, and given EtOH for the first four sessions (acquisition), artificial cerebrospinal fluid for sessions 5 and 6 (extinction), and EtOH again in session 7 (reinstatement). Responding on the active lever produced a 100-nl injection of the infusate. RESULTS: P rats self-infused 75 to 200 mg/100 ml EtOH and demonstrated lever discrimination, whereas Wistar rats reliably self-infused only 150 and 200 mg/100 ml EtOH. Both P and Wistar rats reduced responding on the active lever when artificial cerebrospinal fluid (aCSF) was substituted for EtOH and reinstated responding in session 7 when EtOH was restored, although P rats demonstrated a very robust enhancement of responding for 100 and 150 mg/100 ml EtOH, and this was not found for Wistar rats. CONCLUSIONS: These results suggest that, compared with Wistar rats, the posterior VTA of P rats was more sensitive to the reinforcing effects of EtOH. Furthermore, the reinstatement data suggest that the posterior VTA of P rats underwent neuronal alterations as a result of prior EtOH exposure and extinction that changed the reinforcing effects of EtOH within this region. SN - 0145-6008 UR - https://www.unboundmedicine.com/medline/citation/15318120/Comparison_of_intracranial_self_administration_of_ethanol_within_the_posterior_ventral_tegmental_area_between_alcohol_preferring_and_Wistar_rats_ DB - PRIME DP - Unbound Medicine ER -