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An increase in the absolute count of CD56dimCD16+CD69+ NK cells in the peripheral blood is associated with a poorer IVF treatment and pregnancy outcome.
Hum Reprod 2004; 19(10):2395-400HR

Abstract

BACKGROUND

Our aim was to evaluate the effect of the absolute count of the activation marker (CD69), IgG Fc receptor (CD16) and inhibitor marker (CD94) expression on peripheral blood natural killer (NK) cells on implantation and miscarriage rates after IVF treatment.

METHODS

Prospective observational study of 138 randomly selected women who underwent IVF treatment from December 2002 to September 2003. NK cells were identified as CD56(+) (dim + bright) and CD3(-) by flow cytometry. The absolute counts of the CD69(+), CD16(+) and CD94(+)expressing NK cells were recorded and their relation to IVF treatment outcome and miscarriage rate was analysed.

RESULTS

The mean (+/-SD) absolute count of the CD56(dim)CD16(+)CD69(+) NK cells for women who had a successful ongoing pregnancy was 0.61 x 10(6)/l (+/-0.31). For those women who failed to achieve a pregnancy, the mean value of the absolute count of CD56(dim)CD16(+)D69(+) NK cells was significantly (P=0.003) higher at 1.66 x 10(6)/l (+/-0.52). The absolute count of CD56(dim)CD16(+)CD94(+) and CD56(dim)CD16(+) NK cells did not show any statistically significant differences between those women with successful and failed IVF treatment. Receiver operating characteristic (ROC) curve analysis was performed to select a CD69 threshold for further statistical analysis. The implantation rate (IR) was significantly lower (13.1%) and miscarriage rate (MR) was significantly higher (66.7%) for women with an absolute CD56(dim)CD16(+)CD69(+) NK cell count of >1.0 x 10(6)/l compared to women with count below this value (IR 28.2% and MR 16.7%). Further analysis of the absolute count of CD56(bright)CD69(+) and CD56(bright)CD94(+) NK cells did not show any significant difference between those women with successful and failed IVF treatment.

CONCLUSIONS

An increase in the absolute count of activated NK cells (CD56(dim)CD16(+)CD69(+)) in the peripheral blood is associated with a reduced rate of embryo implantation in IVF treatment. Furthermore, women with high CD56(dim)CD16(+)CD69(+) peripheral blood NK cell absolute count, who are able to achieve pregnancy, have a significantly higher miscarriage rate.

Authors+Show Affiliations

Lister Fertility Clinic, Lister Hospital, Chelsea Bridge Road, London SW1W 8RH, UK. mythum@doctors.net.ukNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15319390

Citation

Thum, M Y., et al. "An Increase in the Absolute Count of CD56dimCD16+CD69+ NK Cells in the Peripheral Blood Is Associated With a Poorer IVF Treatment and Pregnancy Outcome." Human Reproduction (Oxford, England), vol. 19, no. 10, 2004, pp. 2395-400.
Thum MY, Bhaskaran S, Abdalla HI, et al. An increase in the absolute count of CD56dimCD16+CD69+ NK cells in the peripheral blood is associated with a poorer IVF treatment and pregnancy outcome. Hum Reprod. 2004;19(10):2395-400.
Thum, M. Y., Bhaskaran, S., Abdalla, H. I., Ford, B., Sumar, N., Shehata, H., & Bansal, A. S. (2004). An increase in the absolute count of CD56dimCD16+CD69+ NK cells in the peripheral blood is associated with a poorer IVF treatment and pregnancy outcome. Human Reproduction (Oxford, England), 19(10), pp. 2395-400.
Thum MY, et al. An Increase in the Absolute Count of CD56dimCD16+CD69+ NK Cells in the Peripheral Blood Is Associated With a Poorer IVF Treatment and Pregnancy Outcome. Hum Reprod. 2004;19(10):2395-400. PubMed PMID: 15319390.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - An increase in the absolute count of CD56dimCD16+CD69+ NK cells in the peripheral blood is associated with a poorer IVF treatment and pregnancy outcome. AU - Thum,M Y, AU - Bhaskaran,S, AU - Abdalla,H I, AU - Ford,B, AU - Sumar,N, AU - Shehata,H, AU - Bansal,A S, Y1 - 2004/08/19/ PY - 2004/8/21/pubmed PY - 2005/3/9/medline PY - 2004/8/21/entrez SP - 2395 EP - 400 JF - Human reproduction (Oxford, England) JO - Hum. Reprod. VL - 19 IS - 10 N2 - BACKGROUND: Our aim was to evaluate the effect of the absolute count of the activation marker (CD69), IgG Fc receptor (CD16) and inhibitor marker (CD94) expression on peripheral blood natural killer (NK) cells on implantation and miscarriage rates after IVF treatment. METHODS: Prospective observational study of 138 randomly selected women who underwent IVF treatment from December 2002 to September 2003. NK cells were identified as CD56(+) (dim + bright) and CD3(-) by flow cytometry. The absolute counts of the CD69(+), CD16(+) and CD94(+)expressing NK cells were recorded and their relation to IVF treatment outcome and miscarriage rate was analysed. RESULTS: The mean (+/-SD) absolute count of the CD56(dim)CD16(+)CD69(+) NK cells for women who had a successful ongoing pregnancy was 0.61 x 10(6)/l (+/-0.31). For those women who failed to achieve a pregnancy, the mean value of the absolute count of CD56(dim)CD16(+)D69(+) NK cells was significantly (P=0.003) higher at 1.66 x 10(6)/l (+/-0.52). The absolute count of CD56(dim)CD16(+)CD94(+) and CD56(dim)CD16(+) NK cells did not show any statistically significant differences between those women with successful and failed IVF treatment. Receiver operating characteristic (ROC) curve analysis was performed to select a CD69 threshold for further statistical analysis. The implantation rate (IR) was significantly lower (13.1%) and miscarriage rate (MR) was significantly higher (66.7%) for women with an absolute CD56(dim)CD16(+)CD69(+) NK cell count of >1.0 x 10(6)/l compared to women with count below this value (IR 28.2% and MR 16.7%). Further analysis of the absolute count of CD56(bright)CD69(+) and CD56(bright)CD94(+) NK cells did not show any significant difference between those women with successful and failed IVF treatment. CONCLUSIONS: An increase in the absolute count of activated NK cells (CD56(dim)CD16(+)CD69(+)) in the peripheral blood is associated with a reduced rate of embryo implantation in IVF treatment. Furthermore, women with high CD56(dim)CD16(+)CD69(+) peripheral blood NK cell absolute count, who are able to achieve pregnancy, have a significantly higher miscarriage rate. SN - 0268-1161 UR - https://www.unboundmedicine.com/medline/citation/15319390/An_increase_in_the_absolute_count_of_CD56dimCD16+CD69+_NK_cells_in_the_peripheral_blood_is_associated_with_a_poorer_IVF_treatment_and_pregnancy_outcome_ L2 - https://academic.oup.com/humrep/article-lookup/doi/10.1093/humrep/deh378 DB - PRIME DP - Unbound Medicine ER -