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In-vitro trypanocidal activity evaluation of crude extract and isolated compounds from Baccharis dracunculifolia D.C. (Asteraceae).
J Pharm Pharmacol. 2004 Sep; 56(9):1195-9.JP

Abstract

We have performed a trypanocidal bioactivity-guided study of Baccharis dracunculifolia (Asteraceae), the main botanical origin of Brazilian green propolis. The leaf rinse extract of B. dracunculifolia, at a concentration of 3.0 mg mL(-1), displayed 100% lysis of trypomastigote forms of the Y strain of Trypanosoma cruzi (2 x 10(6) parasites mL(-1)). The chromatographic fractionation of the leaf rinse, using several techniques, afforded the isolation of the compounds isosakuranetin (1), aromadendrin-4'-methylether (2), baccharis oxide (3), ferulic acid (4), dihydrocinnamic acid (5), 3-prenyl-4-(dihydrocinnamoyloxy)-cinnamic acid (6), and friedelanol (7). The chemical structures of all compounds were established by UV-vis, (1)H and (13)CNMR data analysis in comparison with the literature. Compounds 1 and 3 were the most active in the trypanocidal assay, showing IC50 values (inhibitory concentration required for 50% inhibition) of 247.6 and 249.8 microM, respectively. Compounds 2, 4, and 6 displayed moderate activity, whilst compounds 5 and 7 were inactive.

Authors+Show Affiliations

da Silva Filho AA
Departamento de Ciências Farmacêuticas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Avenida do café s/n, 14040-903 Ribeirão Preto, SP, Brazil.
Pires Bueno PC
No affiliation info available
Gregório LE
No affiliation info available
Andrade e Silva ML
No affiliation info available
Albuquerque S
No affiliation info available
Bastos JK
No affiliation info available

MeSH

AnimalsBaccharisDose-Response Relationship, DrugDrug Evaluation, PreclinicalMicePlant ExtractsPlant LeavesPlant RootsTrypanocidal AgentsTrypanosoma cruzi

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15324490

Citation

da Silva Filho, Ademar A., et al. "In-vitro Trypanocidal Activity Evaluation of Crude Extract and Isolated Compounds From Baccharis Dracunculifolia D.C. (Asteraceae)." The Journal of Pharmacy and Pharmacology, vol. 56, no. 9, 2004, pp. 1195-9.
da Silva Filho AA, Pires Bueno PC, Gregório LE, et al. In-vitro trypanocidal activity evaluation of crude extract and isolated compounds from Baccharis dracunculifolia D.C. (Asteraceae). J Pharm Pharmacol. 2004;56(9):1195-9.
da Silva Filho, A. A., Pires Bueno, P. C., Gregório, L. E., Andrade e Silva, M. L., Albuquerque, S., & Bastos, J. K. (2004). In-vitro trypanocidal activity evaluation of crude extract and isolated compounds from Baccharis dracunculifolia D.C. (Asteraceae). The Journal of Pharmacy and Pharmacology, 56(9), 1195-9.
da Silva Filho AA, et al. In-vitro Trypanocidal Activity Evaluation of Crude Extract and Isolated Compounds From Baccharis Dracunculifolia D.C. (Asteraceae). J Pharm Pharmacol. 2004;56(9):1195-9. PubMed PMID: 15324490.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In-vitro trypanocidal activity evaluation of crude extract and isolated compounds from Baccharis dracunculifolia D.C. (Asteraceae). AU - da Silva Filho,Ademar A, AU - Pires Bueno,Paula C, AU - Gregório,Luís E, AU - Andrade e Silva,Márcio L, AU - Albuquerque,Sérgio, AU - Bastos,Jairo K, PY - 2004/8/25/pubmed PY - 2004/12/22/medline PY - 2004/8/25/entrez SP - 1195 EP - 9 JF - The Journal of pharmacy and pharmacology JO - J Pharm Pharmacol VL - 56 IS - 9 N2 - We have performed a trypanocidal bioactivity-guided study of Baccharis dracunculifolia (Asteraceae), the main botanical origin of Brazilian green propolis. The leaf rinse extract of B. dracunculifolia, at a concentration of 3.0 mg mL(-1), displayed 100% lysis of trypomastigote forms of the Y strain of Trypanosoma cruzi (2 x 10(6) parasites mL(-1)). The chromatographic fractionation of the leaf rinse, using several techniques, afforded the isolation of the compounds isosakuranetin (1), aromadendrin-4'-methylether (2), baccharis oxide (3), ferulic acid (4), dihydrocinnamic acid (5), 3-prenyl-4-(dihydrocinnamoyloxy)-cinnamic acid (6), and friedelanol (7). The chemical structures of all compounds were established by UV-vis, (1)H and (13)CNMR data analysis in comparison with the literature. Compounds 1 and 3 were the most active in the trypanocidal assay, showing IC50 values (inhibitory concentration required for 50% inhibition) of 247.6 and 249.8 microM, respectively. Compounds 2, 4, and 6 displayed moderate activity, whilst compounds 5 and 7 were inactive. SN - 0022-3573 UR - https://www.unboundmedicine.com/medline/citation/15324490/In_vitro_trypanocidal_activity_evaluation_of_crude_extract_and_isolated_compounds_from_Baccharis_dracunculifolia_D_C___Asteraceae__ DB - PRIME DP - Unbound Medicine ER -