Tags

Type your tag names separated by a space and hit enter

Selective uptake of high density lipoproteins cholesteryl ester in the dog, a species lacking in cholesteryl ester transfer protein activity; An in vivo approach using stable isotopes.
Comp Biochem Physiol B Biochem Mol Biol. 2004 Aug; 138(4):339-45.CB

Abstract

Amongst the processes involved in the reverse cholesterol transport (RCT) from organs to liver, including high density lipoproteins-apolipoprotein AI (HDL-apoAI) dependent tissue uptake and cholesteryl ester transfer protein (CETP)-mediated transfers, the selective uptake of cholesteryl ester (CE) is of increasing interest through its antiatherogenic role. The purpose of this report is to develop a simple protocol allowing study of this process in an animal model with easier quantification of CE selective uptake. The dog was chosen essentially because this animal has a low CETP activity and an appropriate size to conduce a kinetic study. Tracer kinetics were performed to estimate in vivo the contributions of the pathways involved in HDL-CE turnover in dogs. Stable isotopes, 13C-acetate and D3-leucine as labeled precursors of CE and apoAI, were infused to fasting dogs. Isotopic enrichments were monitored in plasma unesterified cholesterol and in HDL-CE and apoAI by mass spectrometry. Kinetics were analyzed using compartmental modeling. Results concerned the measurement of the activity of cholesterol esterification (0.13+/-0.032 h(-1)), rate of HDL-apoAI catabolism (0.024+/-0.012 h(-1)), HDL-CE turnover (0.062+/-0.010 h(-1)) and CE selective uptake (0.038+/-0.014 h(-1)). Our results show that CE in dogs is mainly eliminated by selective uptake of HDL-CE (60% of HDL-CE turnover), unlike in other species studied by similar methods in our laboratory. This study shows that among species used to analyze cholesterol metabolism, the dog appears to be the animal in whom HDL-CE selective uptake represents the largest part of HDL-CE turnover.

Authors+Show Affiliations

Centre de Recherche en Nutrition Humaine, INSERM U539, CHU Nantes, 1 place Alexis Ricordeau, 44093 Nantes 01, France. Khadija.Ouguerram@sante.univ-nantes.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15325333

Citation

Ouguerram, Khadija, et al. "Selective Uptake of High Density Lipoproteins Cholesteryl Ester in the Dog, a Species Lacking in Cholesteryl Ester Transfer Protein Activity; an in Vivo Approach Using Stable Isotopes." Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, vol. 138, no. 4, 2004, pp. 339-45.
Ouguerram K, Nguyen P, Krempf M, et al. Selective uptake of high density lipoproteins cholesteryl ester in the dog, a species lacking in cholesteryl ester transfer protein activity; An in vivo approach using stable isotopes. Comp Biochem Physiol B Biochem Mol Biol. 2004;138(4):339-45.
Ouguerram, K., Nguyen, P., Krempf, M., Pouteau, E., Briand, F., Bailhache, E., & Magot, T. (2004). Selective uptake of high density lipoproteins cholesteryl ester in the dog, a species lacking in cholesteryl ester transfer protein activity; An in vivo approach using stable isotopes. Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, 138(4), 339-45.
Ouguerram K, et al. Selective Uptake of High Density Lipoproteins Cholesteryl Ester in the Dog, a Species Lacking in Cholesteryl Ester Transfer Protein Activity; an in Vivo Approach Using Stable Isotopes. Comp Biochem Physiol B Biochem Mol Biol. 2004;138(4):339-45. PubMed PMID: 15325333.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Selective uptake of high density lipoproteins cholesteryl ester in the dog, a species lacking in cholesteryl ester transfer protein activity; An in vivo approach using stable isotopes. AU - Ouguerram,Khadija, AU - Nguyen,Patrick, AU - Krempf,Michel, AU - Pouteau,Etienne, AU - Briand,François, AU - Bailhache,Edwige, AU - Magot,Thierry, PY - 2003/12/16/received PY - 2004/04/09/revised PY - 2004/04/13/accepted PY - 2004/8/25/pubmed PY - 2005/3/10/medline PY - 2004/8/25/entrez SP - 339 EP - 45 JF - Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology JO - Comp Biochem Physiol B Biochem Mol Biol VL - 138 IS - 4 N2 - Amongst the processes involved in the reverse cholesterol transport (RCT) from organs to liver, including high density lipoproteins-apolipoprotein AI (HDL-apoAI) dependent tissue uptake and cholesteryl ester transfer protein (CETP)-mediated transfers, the selective uptake of cholesteryl ester (CE) is of increasing interest through its antiatherogenic role. The purpose of this report is to develop a simple protocol allowing study of this process in an animal model with easier quantification of CE selective uptake. The dog was chosen essentially because this animal has a low CETP activity and an appropriate size to conduce a kinetic study. Tracer kinetics were performed to estimate in vivo the contributions of the pathways involved in HDL-CE turnover in dogs. Stable isotopes, 13C-acetate and D3-leucine as labeled precursors of CE and apoAI, were infused to fasting dogs. Isotopic enrichments were monitored in plasma unesterified cholesterol and in HDL-CE and apoAI by mass spectrometry. Kinetics were analyzed using compartmental modeling. Results concerned the measurement of the activity of cholesterol esterification (0.13+/-0.032 h(-1)), rate of HDL-apoAI catabolism (0.024+/-0.012 h(-1)), HDL-CE turnover (0.062+/-0.010 h(-1)) and CE selective uptake (0.038+/-0.014 h(-1)). Our results show that CE in dogs is mainly eliminated by selective uptake of HDL-CE (60% of HDL-CE turnover), unlike in other species studied by similar methods in our laboratory. This study shows that among species used to analyze cholesterol metabolism, the dog appears to be the animal in whom HDL-CE selective uptake represents the largest part of HDL-CE turnover. SN - 1096-4959 UR - https://www.unboundmedicine.com/medline/citation/15325333/Selective_uptake_of_high_density_lipoproteins_cholesteryl_ester_in_the_dog_a_species_lacking_in_cholesteryl_ester_transfer_protein_activity L2 - https://linkinghub.elsevier.com/retrieve/pii/S1096495904001332 DB - PRIME DP - Unbound Medicine ER -