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Vital role of the itch-scratch response in development of spontaneous dermatitis in NC/Nga mice.
Br J Dermatol. 2004 Aug; 151(2):335-45.BJ

Abstract

BACKGROUND

The itch sensation and the resultant response, scratching, are important symptoms of atopic dermatitis (AD) and have a significant impact on the quality of life of affected patients. However, the influence of the itch-scratch response on the pathology of AD has not been precisely elucidated.

OBJECTIVES

To investigate the role of scratching behaviour in the development of spontaneous dermatitis using conventionally raised NC/Nga mice (Conv-NC mice), which are known to be an animal model for human AD.

METHODS

Capsaicin-sensitive sensory nerves of the mice were ablated by neonatal capsaicin treatment (Cap-NC mice), and the development of spontaneous dermatitis in the Cap-NC mice was compared chronologically with that in Conv-NC mice.

RESULTS

Scratching behaviour was almost completely prevented in Cap-NC mice raised for 84 days under conventional conditions, and the development of dermatitis and elevation of the serum IgE level were significantly suppressed. Histological analysis revealed that the numbers of infiltrating eosinophils and mast cells in the lesional skin of Cap-NC mice were lower than those in Conv-NC mice. Immunological studies showed that the capability of spleen T cells to produce both T-helper (Th) 1 (interferon-gamma) and Th2 [interleukin (IL)-5 and IL-13] cytokines was diminished in Cap-NC mice. Furthermore, serum levels of IL-18 were approximately twice higher in Conv-NC mice than in Cap-NC mice.

CONCLUSIONS

These observations suggest that scratching behaviour contributes to the development of dermatitis by enhancing various immunological responses in the murine AD model, implying that prevention of the itch sensation and/or itch-associated scratching behaviour is an effective treatment for AD.

Authors+Show Affiliations

R&D Laboratories, Nippon Organon K.K., 5-90 Tomobuchi-cho 1-chome, Miyakojima-Ku, Osaka 534-0016, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15327540

Citation

Mihara, K, et al. "Vital Role of the Itch-scratch Response in Development of Spontaneous Dermatitis in NC/Nga Mice." The British Journal of Dermatology, vol. 151, no. 2, 2004, pp. 335-45.
Mihara K, Kuratani K, Matsui T, et al. Vital role of the itch-scratch response in development of spontaneous dermatitis in NC/Nga mice. Br J Dermatol. 2004;151(2):335-45.
Mihara, K., Kuratani, K., Matsui, T., Nakamura, M., & Yokota, K. (2004). Vital role of the itch-scratch response in development of spontaneous dermatitis in NC/Nga mice. The British Journal of Dermatology, 151(2), 335-45.
Mihara K, et al. Vital Role of the Itch-scratch Response in Development of Spontaneous Dermatitis in NC/Nga Mice. Br J Dermatol. 2004;151(2):335-45. PubMed PMID: 15327540.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Vital role of the itch-scratch response in development of spontaneous dermatitis in NC/Nga mice. AU - Mihara,K, AU - Kuratani,K, AU - Matsui,T, AU - Nakamura,M, AU - Yokota,K, PY - 2004/8/26/pubmed PY - 2004/12/17/medline PY - 2004/8/26/entrez SP - 335 EP - 45 JF - The British journal of dermatology JO - Br J Dermatol VL - 151 IS - 2 N2 - BACKGROUND: The itch sensation and the resultant response, scratching, are important symptoms of atopic dermatitis (AD) and have a significant impact on the quality of life of affected patients. However, the influence of the itch-scratch response on the pathology of AD has not been precisely elucidated. OBJECTIVES: To investigate the role of scratching behaviour in the development of spontaneous dermatitis using conventionally raised NC/Nga mice (Conv-NC mice), which are known to be an animal model for human AD. METHODS: Capsaicin-sensitive sensory nerves of the mice were ablated by neonatal capsaicin treatment (Cap-NC mice), and the development of spontaneous dermatitis in the Cap-NC mice was compared chronologically with that in Conv-NC mice. RESULTS: Scratching behaviour was almost completely prevented in Cap-NC mice raised for 84 days under conventional conditions, and the development of dermatitis and elevation of the serum IgE level were significantly suppressed. Histological analysis revealed that the numbers of infiltrating eosinophils and mast cells in the lesional skin of Cap-NC mice were lower than those in Conv-NC mice. Immunological studies showed that the capability of spleen T cells to produce both T-helper (Th) 1 (interferon-gamma) and Th2 [interleukin (IL)-5 and IL-13] cytokines was diminished in Cap-NC mice. Furthermore, serum levels of IL-18 were approximately twice higher in Conv-NC mice than in Cap-NC mice. CONCLUSIONS: These observations suggest that scratching behaviour contributes to the development of dermatitis by enhancing various immunological responses in the murine AD model, implying that prevention of the itch sensation and/or itch-associated scratching behaviour is an effective treatment for AD. SN - 0007-0963 UR - https://www.unboundmedicine.com/medline/citation/15327540/Vital_role_of_the_itch_scratch_response_in_development_of_spontaneous_dermatitis_in_NC/Nga_mice_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0007-0963&date=2004&volume=151&issue=2&spage=335 DB - PRIME DP - Unbound Medicine ER -