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Lack of evidence for significant neuronal loss in laminae I-III of the spinal dorsal horn of the rat in the chronic constriction injury model.
Pain 2004; 111(1-2):144-50PAIN

Abstract

Peripheral nerve injury leads to structural and functional changes in the spinal dorsal horn, and these are thought to be involved in the development of neuropathic pain. In the chronic constriction injury (CCI) model, abnormal 'dark' neurons and apoptotic nuclei have been observed in laminae I-III of the dorsal horn in the territory innervated by the injured sciatic nerve. These findings have been taken as evidence that there is significant neuronal death in this model, and it has been suggested that loss of inhibition resulting from death of GABAergic inhibitory interneurons contributes to the neuropathic pain. However, loss of neurons from the dorsal horn has not been directly demonstrated in neuropathic models, even though this issue is of considerable importance for our understanding of the mechanisms that underlie neuropathic pain. In this study, we have looked for evidence of neuronal death by using a stereological method (the optical disector) with NeuN-immunostaining, and examining spinal cords of naïve rats, and of rats that had undergone CCI or sham operations. All of the CCI animals showed clear signs of thermal hyperalgesia. However, the numbers of neurons in laminae I-III of the ipsilateral dorsal horn in these animals did not differ significantly from those on the contralateral side, nor from those of sham-operated or naïve animals. These results do not, therefore, support the suggestion that there is significant neuronal death in the dorsal horn in this model.

Authors+Show Affiliations

Spinal Cord Group, Institute of Biomedical and Life Sciences, University of Glasgow, West Medical Building, Glasgow G12 8QQ, UK. e.polgar@bio.gla.ac.ukNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15327818

Citation

Polgár, E, et al. "Lack of Evidence for Significant Neuronal Loss in Laminae I-III of the Spinal Dorsal Horn of the Rat in the Chronic Constriction Injury Model." Pain, vol. 111, no. 1-2, 2004, pp. 144-50.
Polgár E, Gray S, Riddell JS, et al. Lack of evidence for significant neuronal loss in laminae I-III of the spinal dorsal horn of the rat in the chronic constriction injury model. Pain. 2004;111(1-2):144-50.
Polgár, E., Gray, S., Riddell, J. S., & Todd, A. J. (2004). Lack of evidence for significant neuronal loss in laminae I-III of the spinal dorsal horn of the rat in the chronic constriction injury model. Pain, 111(1-2), pp. 144-50.
Polgár E, et al. Lack of Evidence for Significant Neuronal Loss in Laminae I-III of the Spinal Dorsal Horn of the Rat in the Chronic Constriction Injury Model. Pain. 2004;111(1-2):144-50. PubMed PMID: 15327818.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Lack of evidence for significant neuronal loss in laminae I-III of the spinal dorsal horn of the rat in the chronic constriction injury model. AU - Polgár,E, AU - Gray,S, AU - Riddell,J S, AU - Todd,A J, PY - 2004/01/22/received PY - 2004/05/12/revised PY - 2004/06/07/accepted PY - 2004/8/26/pubmed PY - 2004/11/13/medline PY - 2004/8/26/entrez SP - 144 EP - 50 JF - Pain JO - Pain VL - 111 IS - 1-2 N2 - Peripheral nerve injury leads to structural and functional changes in the spinal dorsal horn, and these are thought to be involved in the development of neuropathic pain. In the chronic constriction injury (CCI) model, abnormal 'dark' neurons and apoptotic nuclei have been observed in laminae I-III of the dorsal horn in the territory innervated by the injured sciatic nerve. These findings have been taken as evidence that there is significant neuronal death in this model, and it has been suggested that loss of inhibition resulting from death of GABAergic inhibitory interneurons contributes to the neuropathic pain. However, loss of neurons from the dorsal horn has not been directly demonstrated in neuropathic models, even though this issue is of considerable importance for our understanding of the mechanisms that underlie neuropathic pain. In this study, we have looked for evidence of neuronal death by using a stereological method (the optical disector) with NeuN-immunostaining, and examining spinal cords of naïve rats, and of rats that had undergone CCI or sham operations. All of the CCI animals showed clear signs of thermal hyperalgesia. However, the numbers of neurons in laminae I-III of the ipsilateral dorsal horn in these animals did not differ significantly from those on the contralateral side, nor from those of sham-operated or naïve animals. These results do not, therefore, support the suggestion that there is significant neuronal death in the dorsal horn in this model. SN - 0304-3959 UR - https://www.unboundmedicine.com/medline/citation/15327818/Lack_of_evidence_for_significant_neuronal_loss_in_laminae_I_III_of_the_spinal_dorsal_horn_of_the_rat_in_the_chronic_constriction_injury_model_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0304395904002994 DB - PRIME DP - Unbound Medicine ER -