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Involvement of 5-HT1B receptors within the ventral tegmental area in regulation of mesolimbic dopaminergic neuronal activity via GABA mechanisms: a study with dual-probe microdialysis.
Brain Res. 2004 Sep 17; 1021(1):82-91.BR

Abstract

This study was designed to assess the involvement of 5-HT1B receptors within the ventral tegmental area (VTA) in the regulation of mesolimbic dopaminergic transmission. Dual-probe microdialysis was performed in freely moving adult Sprague-Dawley rats with one probe within the VTA and the other within the ipsilateral nucleus accumbens (NACC). Drugs were administered into the VTA via retrograde dialysis. Dialysates from both the VTA and the NAC were collected for determination of dopamine (DA) and gamma-aminobutyric acid (GABA) by high-performance liquid chromatography with electrochemical detection. Intra-tegmental infusion of CP 93129 (20, 40, and 80 microM), a 5-HT1B receptor agonist, increased extracellular DA concentrations in a concentration-dependent manner not only in the NACC but also in the VTA, indicating increased mesolimbic DA neuron activity. Administration of CP 93129 at 80 microM into the VTA also significantly decreased extracellular GABA concentrations in this region. Co-infusion of the 5-HT1B receptor antagonist SB 216641 (10 microM), but not the 5-HT1A receptor antagonist WAY 100635 (10 microM) or the 5-HT1D/1A receptor antagonist BRL 15572 (10 microM), antagonized not only the effects of intra-tegmental CP 93129 (80 microM) on VTA DA and NAC DA but also on VTA GABA. The results suggest that activation of VTA 5-HT1B receptors increases mesolimbic DA neuron activities. The increased DA neuron activity may be associated, at least in part, with the 5-HT1B receptor-mediated inhibition of VTA GABA release.

Authors+Show Affiliations

Department of Biomedical and Therapeutic Sciences, University of Illinois College of Medicine at Peoria, Peoria, IL 61656, USA. QSY@UIC.EDUNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

15328035

Citation

Yan, Qing-Shan, et al. "Involvement of 5-HT1B Receptors Within the Ventral Tegmental Area in Regulation of Mesolimbic Dopaminergic Neuronal Activity Via GABA Mechanisms: a Study With Dual-probe Microdialysis." Brain Research, vol. 1021, no. 1, 2004, pp. 82-91.
Yan QS, Zheng SZ, Yan SE. Involvement of 5-HT1B receptors within the ventral tegmental area in regulation of mesolimbic dopaminergic neuronal activity via GABA mechanisms: a study with dual-probe microdialysis. Brain Res. 2004;1021(1):82-91.
Yan, Q. S., Zheng, S. Z., & Yan, S. E. (2004). Involvement of 5-HT1B receptors within the ventral tegmental area in regulation of mesolimbic dopaminergic neuronal activity via GABA mechanisms: a study with dual-probe microdialysis. Brain Research, 1021(1), 82-91.
Yan QS, Zheng SZ, Yan SE. Involvement of 5-HT1B Receptors Within the Ventral Tegmental Area in Regulation of Mesolimbic Dopaminergic Neuronal Activity Via GABA Mechanisms: a Study With Dual-probe Microdialysis. Brain Res. 2004 Sep 17;1021(1):82-91. PubMed PMID: 15328035.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Involvement of 5-HT1B receptors within the ventral tegmental area in regulation of mesolimbic dopaminergic neuronal activity via GABA mechanisms: a study with dual-probe microdialysis. AU - Yan,Qing-Shan, AU - Zheng,Shi-Zhong, AU - Yan,Shu-E, PY - 2004/06/27/accepted PY - 2004/8/26/pubmed PY - 2004/11/9/medline PY - 2004/8/26/entrez SP - 82 EP - 91 JF - Brain research JO - Brain Res. VL - 1021 IS - 1 N2 - This study was designed to assess the involvement of 5-HT1B receptors within the ventral tegmental area (VTA) in the regulation of mesolimbic dopaminergic transmission. Dual-probe microdialysis was performed in freely moving adult Sprague-Dawley rats with one probe within the VTA and the other within the ipsilateral nucleus accumbens (NACC). Drugs were administered into the VTA via retrograde dialysis. Dialysates from both the VTA and the NAC were collected for determination of dopamine (DA) and gamma-aminobutyric acid (GABA) by high-performance liquid chromatography with electrochemical detection. Intra-tegmental infusion of CP 93129 (20, 40, and 80 microM), a 5-HT1B receptor agonist, increased extracellular DA concentrations in a concentration-dependent manner not only in the NACC but also in the VTA, indicating increased mesolimbic DA neuron activity. Administration of CP 93129 at 80 microM into the VTA also significantly decreased extracellular GABA concentrations in this region. Co-infusion of the 5-HT1B receptor antagonist SB 216641 (10 microM), but not the 5-HT1A receptor antagonist WAY 100635 (10 microM) or the 5-HT1D/1A receptor antagonist BRL 15572 (10 microM), antagonized not only the effects of intra-tegmental CP 93129 (80 microM) on VTA DA and NAC DA but also on VTA GABA. The results suggest that activation of VTA 5-HT1B receptors increases mesolimbic DA neuron activities. The increased DA neuron activity may be associated, at least in part, with the 5-HT1B receptor-mediated inhibition of VTA GABA release. SN - 0006-8993 UR - https://www.unboundmedicine.com/medline/citation/15328035/Involvement_of_5_HT1B_receptors_within_the_ventral_tegmental_area_in_regulation_of_mesolimbic_dopaminergic_neuronal_activity_via_GABA_mechanisms:_a_study_with_dual_probe_microdialysis_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0006-8993(04)01033-9 DB - PRIME DP - Unbound Medicine ER -