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Purinergic component of mechanosensory transduction is increased in a rat model of colitis.
Am J Physiol Gastrointest Liver Physiol. 2004 Sep; 287(3):G647-57.AJ

Abstract

ATP contributes to mechanosensory transduction in the rat colorectum. P2X3 receptors are present on dorsal root ganglia (DRG) neurons that supply this area of the gut. Previous studies have shown an increased role for ATP in inflamed tissues. We aimed to investigate whether an increased purinergic component exists during mechanosensory transduction in a rat model of colitis. An in vitro rat colorectal preparation was used to investigate whether distension increased ATP release and to evaluate the role of purinergic antagonists in distension-evoked sensory discharges in the pelvic nerve in normal and colitis preparations. DRG neuron purinoceptors were also studied. Distension-evoked responses in the colitis model were attenuated to a significantly greater extent by 2',3'-O-trinitrophenyl-ATP and pyridoxyl 5-phosphate 6-azophenyl-2',4'-disulfonic acid. Inflammation caused augmented distension-evoked sensory nerve excitation after application of ATP and alpha,beta-methylene ATP. Single-fiber analysis confirmed that mean firing per unit was increased. Distension-evoked increases in ATP release from epithelial cells were substantially higher. The number of DRG neurons responding to ATP and the number of those staining for the P2X3 receptor, particularly those containing calcitonin gene-related peptide, were increased. Adenosine, after ectoenzymatic breakdown of ATP, is involved to a lesser degree in the longer-lasting distension-evoked sensory discharge, suggesting reduced ATPase activity. It was therefore concluded that ATP has an enhanced role in mechanosensory transduction in the inflamed rat colorectum. The underlying mechanisms appear to involve increased distension-evoked release of ATP as well as an increase in the number of DRG neurons supplying the colorectum expressing P2X3 receptors, especially those containing calcitonin gene-related peptide.

Authors+Show Affiliations

Autonomic Neuroscience Institute, Royal Free and University College School of Medicine, London NW3 2PF, United Kingdom.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15331354

Citation

Wynn, Gregory, et al. "Purinergic Component of Mechanosensory Transduction Is Increased in a Rat Model of Colitis." American Journal of Physiology. Gastrointestinal and Liver Physiology, vol. 287, no. 3, 2004, pp. G647-57.
Wynn G, Ma B, Ruan HZ, et al. Purinergic component of mechanosensory transduction is increased in a rat model of colitis. Am J Physiol Gastrointest Liver Physiol. 2004;287(3):G647-57.
Wynn, G., Ma, B., Ruan, H. Z., & Burnstock, G. (2004). Purinergic component of mechanosensory transduction is increased in a rat model of colitis. American Journal of Physiology. Gastrointestinal and Liver Physiology, 287(3), G647-57.
Wynn G, et al. Purinergic Component of Mechanosensory Transduction Is Increased in a Rat Model of Colitis. Am J Physiol Gastrointest Liver Physiol. 2004;287(3):G647-57. PubMed PMID: 15331354.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Purinergic component of mechanosensory transduction is increased in a rat model of colitis. AU - Wynn,Gregory, AU - Ma,Bei, AU - Ruan,Huai Zhen, AU - Burnstock,Geoffrey, PY - 2004/8/28/pubmed PY - 2004/9/21/medline PY - 2004/8/28/entrez SP - G647 EP - 57 JF - American journal of physiology. Gastrointestinal and liver physiology JO - Am. J. Physiol. Gastrointest. Liver Physiol. VL - 287 IS - 3 N2 - ATP contributes to mechanosensory transduction in the rat colorectum. P2X3 receptors are present on dorsal root ganglia (DRG) neurons that supply this area of the gut. Previous studies have shown an increased role for ATP in inflamed tissues. We aimed to investigate whether an increased purinergic component exists during mechanosensory transduction in a rat model of colitis. An in vitro rat colorectal preparation was used to investigate whether distension increased ATP release and to evaluate the role of purinergic antagonists in distension-evoked sensory discharges in the pelvic nerve in normal and colitis preparations. DRG neuron purinoceptors were also studied. Distension-evoked responses in the colitis model were attenuated to a significantly greater extent by 2',3'-O-trinitrophenyl-ATP and pyridoxyl 5-phosphate 6-azophenyl-2',4'-disulfonic acid. Inflammation caused augmented distension-evoked sensory nerve excitation after application of ATP and alpha,beta-methylene ATP. Single-fiber analysis confirmed that mean firing per unit was increased. Distension-evoked increases in ATP release from epithelial cells were substantially higher. The number of DRG neurons responding to ATP and the number of those staining for the P2X3 receptor, particularly those containing calcitonin gene-related peptide, were increased. Adenosine, after ectoenzymatic breakdown of ATP, is involved to a lesser degree in the longer-lasting distension-evoked sensory discharge, suggesting reduced ATPase activity. It was therefore concluded that ATP has an enhanced role in mechanosensory transduction in the inflamed rat colorectum. The underlying mechanisms appear to involve increased distension-evoked release of ATP as well as an increase in the number of DRG neurons supplying the colorectum expressing P2X3 receptors, especially those containing calcitonin gene-related peptide. SN - 0193-1857 UR - https://www.unboundmedicine.com/medline/citation/15331354/Purinergic_component_of_mechanosensory_transduction_is_increased_in_a_rat_model_of_colitis_ L2 - http://www.physiology.org/doi/full/10.1152/ajpgi.00020.2004?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -