TY - JOUR T1 - Separation, identification, and quantification of selected withanolides in plant extracts of Withania somnifera by HPLC-UV(DAD)--positive ion electrospray ionisation-mass spectrometry. AU - Khajuria,Ravi K, AU - Suri,Krishan A, AU - Gupta,Rajinder K, AU - Satti,Naresh K, AU - Amina,Musarat, AU - Suri,Om P, AU - Qazi,Ghulam N, PY - 2004/9/1/pubmed PY - 2004/9/16/medline PY - 2004/9/1/entrez SP - 541 EP - 6 JF - Journal of separation science JO - J Sep Sci VL - 27 IS - 7-8 N2 - This paper describes a method for separation, identification, and quantification of selected withanolides in Withania somnifera plant extracts by HPLC-UV(DAD)-Mass Spectrometry (HPLC-MS). Withaferin-A (WS-3), 12-deoxywithastramonolide (WS-12DS), Withanolide A (WS-1), and Withanone (WS-2) were used as external standards. The compounds were isolated from Withania somnifera by repeated column chromatography of the root extract and their identity was established by 1H- and 13C-NMR and mass spectral data. The compounds were chromatographed on a Merck (250 x 4.6 mm ID, 5 microm) column and analyzed by Electrospray Ionization on a mass spectrometer in Selected Ion Mode (SIM). For quantification, [M + Na]+ ions were monitored. Linear calibration curves were obtained in the concentration range of 1.50 microg/mL to 6.5 microg/mL. The method was applied successfully to the detection and quantification of the said withanolides in a number of samples. SN - 1615-9306 UR - https://www.unboundmedicine.com/medline/citation/15335037/Separation_identification_and_quantification_of_selected_withanolides_in_plant_extracts_of_Withania_somnifera_by_HPLC_UV_DAD___positive_ion_electrospray_ionisation_mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -