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High numbers of Shiga toxin-producing Escherichia coli found in bovine faeces collected at slaughter in Japan.
FEMS Microbiol Lett. 2004 Sep 01; 238(1):189-97.FM

Abstract

The prevalence and concentration of Shiga toxin-producing Escherichia coli (STEC) in cattle faeces (n=605) at the time of slaughter was studied in Shimane Prefecture, Japan on a monthly basis between April 2000 and March 2001. Screening with stx-PCR determined a prevalence of 37.5%. After analysis of spread faeces and enriched samples on cefixime, tellurite and sorbitol-MacConkey agar using HCl treatment, 114 STEC strains were singly or concomitantly isolated from 97 cattle (15.9%). Of the 605 cattle, 31 (5.1%) harbored O26:H11, O111:H-, O121:H19 or O157:H7, which had the stx1 and/or stx2 and eae and hlyA genes, and 7 (23%) of these 31 cattle were high level carriers that contained these typical STEC at concentrations of 10(5)-10(8) CFU/g of faeces. The predominant serotype was O26:H11 (20 strains) and the second most frequent was O157:H7 (9 strains). Of the 605 cattle, 68 (11.2%) harboured 36 other serotypes and 6 (5.9%) of the 67 cattle were high level carriers. As a comparison between the prevalence of STEC and the faecal pH, it was demonstrated that STEC can be isolated from cattle with a wide range of faecal pH values. The presence of a high-carriage animal at the abattoir increases the potential risk of meat contamination during the slaughtering process, regardless of faecal pH.

Authors+Show Affiliations

Shimane Prefectural Institute of Public Health and Environmental Science, 582 Nishihamasada, Matsue, Shimane 690-0122, Japan. fukushima-hiroshi@pref.shimane.jpNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15336421

Citation

Fukushima, Hiroshi, and Ryotaro Seki. "High Numbers of Shiga Toxin-producing Escherichia Coli Found in Bovine Faeces Collected at Slaughter in Japan." FEMS Microbiology Letters, vol. 238, no. 1, 2004, pp. 189-97.
Fukushima H, Seki R. High numbers of Shiga toxin-producing Escherichia coli found in bovine faeces collected at slaughter in Japan. FEMS Microbiol Lett. 2004;238(1):189-97.
Fukushima, H., & Seki, R. (2004). High numbers of Shiga toxin-producing Escherichia coli found in bovine faeces collected at slaughter in Japan. FEMS Microbiology Letters, 238(1), 189-97.
Fukushima H, Seki R. High Numbers of Shiga Toxin-producing Escherichia Coli Found in Bovine Faeces Collected at Slaughter in Japan. FEMS Microbiol Lett. 2004 Sep 1;238(1):189-97. PubMed PMID: 15336421.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High numbers of Shiga toxin-producing Escherichia coli found in bovine faeces collected at slaughter in Japan. AU - Fukushima,Hiroshi, AU - Seki,Ryotaro, PY - 2004/02/02/received PY - 2004/07/13/revised PY - 2004/07/15/accepted PY - 2004/9/1/pubmed PY - 2005/2/26/medline PY - 2004/9/1/entrez SP - 189 EP - 97 JF - FEMS microbiology letters JO - FEMS Microbiol Lett VL - 238 IS - 1 N2 - The prevalence and concentration of Shiga toxin-producing Escherichia coli (STEC) in cattle faeces (n=605) at the time of slaughter was studied in Shimane Prefecture, Japan on a monthly basis between April 2000 and March 2001. Screening with stx-PCR determined a prevalence of 37.5%. After analysis of spread faeces and enriched samples on cefixime, tellurite and sorbitol-MacConkey agar using HCl treatment, 114 STEC strains were singly or concomitantly isolated from 97 cattle (15.9%). Of the 605 cattle, 31 (5.1%) harbored O26:H11, O111:H-, O121:H19 or O157:H7, which had the stx1 and/or stx2 and eae and hlyA genes, and 7 (23%) of these 31 cattle were high level carriers that contained these typical STEC at concentrations of 10(5)-10(8) CFU/g of faeces. The predominant serotype was O26:H11 (20 strains) and the second most frequent was O157:H7 (9 strains). Of the 605 cattle, 68 (11.2%) harboured 36 other serotypes and 6 (5.9%) of the 67 cattle were high level carriers. As a comparison between the prevalence of STEC and the faecal pH, it was demonstrated that STEC can be isolated from cattle with a wide range of faecal pH values. The presence of a high-carriage animal at the abattoir increases the potential risk of meat contamination during the slaughtering process, regardless of faecal pH. SN - 0378-1097 UR - https://www.unboundmedicine.com/medline/citation/15336421/High_numbers_of_Shiga_toxin_producing_Escherichia_coli_found_in_bovine_faeces_collected_at_slaughter_in_Japan_ L2 - https://academic.oup.com/femsle/article-lookup/doi/10.1016/j.femsle.2004.07.035 DB - PRIME DP - Unbound Medicine ER -