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High-fat enteral nutrition reduces endotoxin, tumor necrosis factor-alpha and gut permeability in bile duct-ligated rats subjected to hemorrhagic shock.
J Hepatol. 2004 Sep; 41(3):377-83.JH

Abstract

BACKGROUND/AIMS

Cholestatic patients are prone to septic complications after major surgery due to an increased susceptibility to endotoxin and hypotension. High-fat enteral nutrition reduces endotoxin after hemorrhagic shock. However, it is unknown whether this nutritional intervention is protective in biliary obstruction. We investigated the effect of high-fat enteral nutrition on endotoxin, tumor necrosis factor-alpha (TNF-alpha) and intestinal permeability in cholestatic rats subjected to hemorrhagic shock.

METHODS

Bile duct-ligated (BDL) rats were fasted or fed with low-fat or high-fat enteral nutrition before hemorrhagic shock. Blood and tissue samples were taken after 90 min.

RESULTS

Plasma endotoxin decreased after hemorrhagic shock in BDL-rats fed with high-fat nutrition compared to fasted (P<0.01) and low-fat treated rats (P<0.05). Additionally, circulating TNF-alpha was reduced in BDL-rats pretreated with high-fat nutrition compared to fasted rats (P<0.01). The increased intestinal permeability to macromolecules was reduced by high-fat enteral nutrition, whereas bacterial translocation did not significantly change. Simultaneously, tight junction distribution in ileum and colon was disrupted in non-treated BDL-rats but remained unchanged in high-fat pretreated BDL-rats.

CONCLUSIONS

High-fat enteral nutrition protects against endotoxin-mediated complications independently of intraluminal bile. These results provide a potential new strategy to prevent endotoxin-mediated complications in cholestatic patients undergoing major surgery.

Authors+Show Affiliations

Department of Surgery, Nutrition and Toxicology Research Institute Maastricht (NUTRIM), University of Maastricht, University Hospital, P.O. Box 616, 6200 MD Maastricht, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15336439

Citation

Luyer, Misha D P., et al. "High-fat Enteral Nutrition Reduces Endotoxin, Tumor Necrosis Factor-alpha and Gut Permeability in Bile Duct-ligated Rats Subjected to Hemorrhagic Shock." Journal of Hepatology, vol. 41, no. 3, 2004, pp. 377-83.
Luyer MD, Buurman WA, Hadfoune M, et al. High-fat enteral nutrition reduces endotoxin, tumor necrosis factor-alpha and gut permeability in bile duct-ligated rats subjected to hemorrhagic shock. J Hepatol. 2004;41(3):377-83.
Luyer, M. D., Buurman, W. A., Hadfoune, M., Jacobs, J. A., Dejong, C. H., & Greve, J. W. (2004). High-fat enteral nutrition reduces endotoxin, tumor necrosis factor-alpha and gut permeability in bile duct-ligated rats subjected to hemorrhagic shock. Journal of Hepatology, 41(3), 377-83.
Luyer MD, et al. High-fat Enteral Nutrition Reduces Endotoxin, Tumor Necrosis Factor-alpha and Gut Permeability in Bile Duct-ligated Rats Subjected to Hemorrhagic Shock. J Hepatol. 2004;41(3):377-83. PubMed PMID: 15336439.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High-fat enteral nutrition reduces endotoxin, tumor necrosis factor-alpha and gut permeability in bile duct-ligated rats subjected to hemorrhagic shock. AU - Luyer,Misha D P, AU - Buurman,Wim A, AU - Hadfoune,M'hamed, AU - Jacobs,Jan A, AU - Dejong,Cornelis H C, AU - Greve,Jan Willem M, PY - 2003/10/31/received PY - 2004/04/27/revised PY - 2004/04/28/accepted PY - 2004/9/1/pubmed PY - 2005/3/2/medline PY - 2004/9/1/entrez SP - 377 EP - 83 JF - Journal of hepatology JO - J Hepatol VL - 41 IS - 3 N2 - BACKGROUND/AIMS: Cholestatic patients are prone to septic complications after major surgery due to an increased susceptibility to endotoxin and hypotension. High-fat enteral nutrition reduces endotoxin after hemorrhagic shock. However, it is unknown whether this nutritional intervention is protective in biliary obstruction. We investigated the effect of high-fat enteral nutrition on endotoxin, tumor necrosis factor-alpha (TNF-alpha) and intestinal permeability in cholestatic rats subjected to hemorrhagic shock. METHODS: Bile duct-ligated (BDL) rats were fasted or fed with low-fat or high-fat enteral nutrition before hemorrhagic shock. Blood and tissue samples were taken after 90 min. RESULTS: Plasma endotoxin decreased after hemorrhagic shock in BDL-rats fed with high-fat nutrition compared to fasted (P<0.01) and low-fat treated rats (P<0.05). Additionally, circulating TNF-alpha was reduced in BDL-rats pretreated with high-fat nutrition compared to fasted rats (P<0.01). The increased intestinal permeability to macromolecules was reduced by high-fat enteral nutrition, whereas bacterial translocation did not significantly change. Simultaneously, tight junction distribution in ileum and colon was disrupted in non-treated BDL-rats but remained unchanged in high-fat pretreated BDL-rats. CONCLUSIONS: High-fat enteral nutrition protects against endotoxin-mediated complications independently of intraluminal bile. These results provide a potential new strategy to prevent endotoxin-mediated complications in cholestatic patients undergoing major surgery. SN - 0168-8278 UR - https://www.unboundmedicine.com/medline/citation/15336439/High_fat_enteral_nutrition_reduces_endotoxin_tumor_necrosis_factor_alpha_and_gut_permeability_in_bile_duct_ligated_rats_subjected_to_hemorrhagic_shock_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0168827804001928 DB - PRIME DP - Unbound Medicine ER -