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Comparison of characteristics of peroxide-conditioned immortal human lens-epithelial cell lines with their murine counterparts.
Exp Eye Res. 2004 Sep; 79(3):411-7.EE

Abstract

Previously, this laboratory has reported the characteristics of murine immortal lens-epithelial cells (alphaTN4-1) conditioned to survive either H2O2 or tertiary butyl hydroperoxide (TBOOH) stress. This communication now describes similar observations upon human HLE-B3 cells. It was found that the human cells are more sensitive to peroxides than their murine counterpart. Similar to the murine cells, conditioning to TBOOH endows the HLE-B3 cells with resistance to H2O2 but unlike the murine cells, conditioning to H2O2 gives the human cells resistance to TBOOH. Furthermore, while withdrawal of TBOOH stress from TBOOH-conditioned alphaTN4-1 cells causes a loss of resistance to this peroxide but not H2O2, with human cells resistance to both peroxides is retained. Examination of the antioxidative defense (AOD) enzyme activities show an extraordinary increase in catalase activity and significant augmentation of most other enzymes assayed in all conditioned human cell lines. In contrast, it was previously found that only catalase and glutathione-S-transferase have considerable increases in activity in the murine lines. However, in most cases, the AOD enzyme activity in murine-control cells is about 2-fold higher than in human control cells. The gene expression of human TBOOH-conditioned (Thum) and control (Chum) lines were also examined utilizing microarray analysis. Surprisingly, no significant change in gene expression was found for any of the prominent AOD enzymes. Such results differ from the response of murine cells where many AOD enzymes have increased expression. These observations suggest while the same AOD enzymes may be utilized in both murine and human lens-epithelial cells, the levels at which they are maintained and the manner in which they are recruited in response to stress may differ.

Authors+Show Affiliations

Department of Ophthalmology, College of Physicians and Surgeons, Columbia University, 630 West 168th Street, New York, NY 10032, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15336504

Citation

Ma, Wanchao, et al. "Comparison of Characteristics of Peroxide-conditioned Immortal Human Lens-epithelial Cell Lines With Their Murine Counterparts." Experimental Eye Research, vol. 79, no. 3, 2004, pp. 411-7.
Ma W, Li D, Sun F, et al. Comparison of characteristics of peroxide-conditioned immortal human lens-epithelial cell lines with their murine counterparts. Exp Eye Res. 2004;79(3):411-7.
Ma, W., Li, D., Sun, F., & Spector, A. (2004). Comparison of characteristics of peroxide-conditioned immortal human lens-epithelial cell lines with their murine counterparts. Experimental Eye Research, 79(3), 411-7.
Ma W, et al. Comparison of Characteristics of Peroxide-conditioned Immortal Human Lens-epithelial Cell Lines With Their Murine Counterparts. Exp Eye Res. 2004;79(3):411-7. PubMed PMID: 15336504.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of characteristics of peroxide-conditioned immortal human lens-epithelial cell lines with their murine counterparts. AU - Ma,Wanchao, AU - Li,Dayu, AU - Sun,Fang, AU - Spector,Abraham, PY - 2004/04/26/received PY - 2004/06/14/accepted PY - 2004/9/1/pubmed PY - 2004/10/13/medline PY - 2004/9/1/entrez SP - 411 EP - 7 JF - Experimental eye research JO - Exp Eye Res VL - 79 IS - 3 N2 - Previously, this laboratory has reported the characteristics of murine immortal lens-epithelial cells (alphaTN4-1) conditioned to survive either H2O2 or tertiary butyl hydroperoxide (TBOOH) stress. This communication now describes similar observations upon human HLE-B3 cells. It was found that the human cells are more sensitive to peroxides than their murine counterpart. Similar to the murine cells, conditioning to TBOOH endows the HLE-B3 cells with resistance to H2O2 but unlike the murine cells, conditioning to H2O2 gives the human cells resistance to TBOOH. Furthermore, while withdrawal of TBOOH stress from TBOOH-conditioned alphaTN4-1 cells causes a loss of resistance to this peroxide but not H2O2, with human cells resistance to both peroxides is retained. Examination of the antioxidative defense (AOD) enzyme activities show an extraordinary increase in catalase activity and significant augmentation of most other enzymes assayed in all conditioned human cell lines. In contrast, it was previously found that only catalase and glutathione-S-transferase have considerable increases in activity in the murine lines. However, in most cases, the AOD enzyme activity in murine-control cells is about 2-fold higher than in human control cells. The gene expression of human TBOOH-conditioned (Thum) and control (Chum) lines were also examined utilizing microarray analysis. Surprisingly, no significant change in gene expression was found for any of the prominent AOD enzymes. Such results differ from the response of murine cells where many AOD enzymes have increased expression. These observations suggest while the same AOD enzymes may be utilized in both murine and human lens-epithelial cells, the levels at which they are maintained and the manner in which they are recruited in response to stress may differ. SN - 0014-4835 UR - https://www.unboundmedicine.com/medline/citation/15336504/Comparison_of_characteristics_of_peroxide_conditioned_immortal_human_lens_epithelial_cell_lines_with_their_murine_counterparts_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014483504001721 DB - PRIME DP - Unbound Medicine ER -