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Refinement of the structures of cell-wall glucans of Schizosaccharomyces pombe by chemical modification and NMR spectroscopy.
Carbohydr Res. 2004 Sep 13; 339(13):2255-65.CR

Abstract

Alkali extraction and methylation analyses in the 1970s revealed that the cell walls of the yeast Schizosaccharomyces pombe contain a (1-->3)-alpha-d-glucan, a (1-->3)-beta-d-glucan, a (1-->6)-beta-d-glucan, and a alpha-galactomannan. To refine the structures of these polysaccharides, cell-wall glucans of S. pombe were extracted, fractionated, and analyzed by NMR spectroscopy. S. pombe cells were treated with 3% NaOH, and alkali-soluble and insoluble fractions were prepared. The alkali-insoluble fraction was treated with 0.5M acetic acid or Zymolyase 100T to yield an alkali-insoluble, acetic acid-insoluble fraction, an alkali-insoluble, Zymolyase-insoluble fraction, and an alkali-insoluble, Zymolyase-soluble fraction. (13)C NMR and 2D-NMR spectra disclosed that the cell wall of S. pombe is composed of three types of glucans, specifically, a (1-->3)-alpha-d-glucan, a (1-->3)-beta-d-glucan, which may either be linear or slightly branched, and a highly branched (1-->6)-beta-d-glucan, in addition to alpha-galactomannan. The highly branched (1-->6)-beta-d-glucan was identified by selective periodate degradation of side-chain glucose as a highly (1-->3)-beta-branched (1-->6)-beta-d-glucan with more branches than that of Saccharomyces cerevisiae. Flexibility of these polysaccharides in the cell wall was analyzed by (13)C NMR spectra in D(2)O. The data collectively indicate that (1-->3)-alpha- and (1-->3)-beta-d-glucans are rigid and contribute to the cell shape, while the highly branched (1-->6)-beta-d-glucan and alpha-galactomannan are flexible.

Authors+Show Affiliations

Division of Chemical and Biological Sciences, Graduate School of Science, 2-8-1, Mejirodai, Bunkyo-ku, Tokyo 112-8681, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

15337454

Citation

Sugawara, Tomoko, et al. "Refinement of the Structures of Cell-wall Glucans of Schizosaccharomyces Pombe By Chemical Modification and NMR Spectroscopy." Carbohydrate Research, vol. 339, no. 13, 2004, pp. 2255-65.
Sugawara T, Takahashi S, Osumi M, et al. Refinement of the structures of cell-wall glucans of Schizosaccharomyces pombe by chemical modification and NMR spectroscopy. Carbohydr Res. 2004;339(13):2255-65.
Sugawara, T., Takahashi, S., Osumi, M., & Ohno, N. (2004). Refinement of the structures of cell-wall glucans of Schizosaccharomyces pombe by chemical modification and NMR spectroscopy. Carbohydrate Research, 339(13), 2255-65.
Sugawara T, et al. Refinement of the Structures of Cell-wall Glucans of Schizosaccharomyces Pombe By Chemical Modification and NMR Spectroscopy. Carbohydr Res. 2004 Sep 13;339(13):2255-65. PubMed PMID: 15337454.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Refinement of the structures of cell-wall glucans of Schizosaccharomyces pombe by chemical modification and NMR spectroscopy. AU - Sugawara,Tomoko, AU - Takahashi,Seizo, AU - Osumi,Masako, AU - Ohno,Naohito, PY - 2004/01/23/received PY - 2004/05/30/accepted PY - 2004/9/1/pubmed PY - 2005/3/30/medline PY - 2004/9/1/entrez SP - 2255 EP - 65 JF - Carbohydrate research JO - Carbohydr Res VL - 339 IS - 13 N2 - Alkali extraction and methylation analyses in the 1970s revealed that the cell walls of the yeast Schizosaccharomyces pombe contain a (1-->3)-alpha-d-glucan, a (1-->3)-beta-d-glucan, a (1-->6)-beta-d-glucan, and a alpha-galactomannan. To refine the structures of these polysaccharides, cell-wall glucans of S. pombe were extracted, fractionated, and analyzed by NMR spectroscopy. S. pombe cells were treated with 3% NaOH, and alkali-soluble and insoluble fractions were prepared. The alkali-insoluble fraction was treated with 0.5M acetic acid or Zymolyase 100T to yield an alkali-insoluble, acetic acid-insoluble fraction, an alkali-insoluble, Zymolyase-insoluble fraction, and an alkali-insoluble, Zymolyase-soluble fraction. (13)C NMR and 2D-NMR spectra disclosed that the cell wall of S. pombe is composed of three types of glucans, specifically, a (1-->3)-alpha-d-glucan, a (1-->3)-beta-d-glucan, which may either be linear or slightly branched, and a highly branched (1-->6)-beta-d-glucan, in addition to alpha-galactomannan. The highly branched (1-->6)-beta-d-glucan was identified by selective periodate degradation of side-chain glucose as a highly (1-->3)-beta-branched (1-->6)-beta-d-glucan with more branches than that of Saccharomyces cerevisiae. Flexibility of these polysaccharides in the cell wall was analyzed by (13)C NMR spectra in D(2)O. The data collectively indicate that (1-->3)-alpha- and (1-->3)-beta-d-glucans are rigid and contribute to the cell shape, while the highly branched (1-->6)-beta-d-glucan and alpha-galactomannan are flexible. SN - 0008-6215 UR - https://www.unboundmedicine.com/medline/citation/15337454/Refinement_of_the_structures_of_cell_wall_glucans_of_Schizosaccharomyces_pombe_by_chemical_modification_and_NMR_spectroscopy_ DB - PRIME DP - Unbound Medicine ER -