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[The ion targets of arrhythmias induced by ouabain and aconitine in guinea pig and rat ventricular myocytes].
Yao Xue Xue Bao. 2004 May; 39(5):328-32.YX

Abstract

AIM

To observe the effects of ouabain and aconitine on APD and ion channels in isolated guinea pig and rat ventricular myocytes; to elucidate the action mechanisms of these two drugs and set up new arrhythmic models on cellular level.

METHODS

In isolated ventricular myocytes of guinea pig and rat, the effects of ouabain and aconitine on APD, ICa-L, Ik, Ito and Ik1 were observed using the whole cell patch clamp technique.

RESULTS

Ouabain (5 micromol x L(-1)) obviously prolonged the APD90, increased ICa-L, decreased Ik and Ik1 in guinea pig ventricular myocytes. Aconitine (1 micromol x L(-1)) lengthened the APD90, increased ICa-L, decreased Ito and increased Ik1 in rat ventricular myocytes.

CONCLUSION

The targets on ouabain- and aconitine-induced arrhythmias included APD, ICa-L, Ik, Ito, and Ik1. APD, ICaL, Ik and Ito must be the powerful ones, both in arrhythmic and antiarrhythmic courses. The ouabain- and aconitine- induced arrhythmic models on cellular level were built to study the antiarrhythmic mechanisms of chemicals and evaluate new drugs. These two new-type models in vitro were stable, liable, repeatable and economic, which were superior to those typical models in vivo.

Authors+Show Affiliations

Gong DM
Department of Pharmacology, Harbin Medical University, Harbin 150086, China. gdmxxl@ems.hrbmu.edu.cn
Shan HL
No affiliation info available
Zhou YH
No affiliation info available
Dong DL
No affiliation info available
Yang BF
No affiliation info available

MeSH

AconitineAction PotentialsAnimalsArrhythmias, CardiacCalcium Channels, L-TypeCell SeparationFemaleGuinea PigsHeart VentriclesMaleMyocytes, CardiacOuabainPotassium Channels, Inwardly RectifyingRatsRats, Wistar

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

15338872

Citation

Gong, Dong-mei, et al. "[The Ion Targets of Arrhythmias Induced By Ouabain and Aconitine in Guinea Pig and Rat Ventricular Myocytes]." Yao Xue Xue Bao = Acta Pharmaceutica Sinica, vol. 39, no. 5, 2004, pp. 328-32.
Gong DM, Shan HL, Zhou YH, et al. [The ion targets of arrhythmias induced by ouabain and aconitine in guinea pig and rat ventricular myocytes]. Yao Xue Xue Bao. 2004;39(5):328-32.
Gong, D. M., Shan, H. L., Zhou, Y. H., Dong, D. L., & Yang, B. F. (2004). [The ion targets of arrhythmias induced by ouabain and aconitine in guinea pig and rat ventricular myocytes]. Yao Xue Xue Bao = Acta Pharmaceutica Sinica, 39(5), 328-32.
Gong DM, et al. [The Ion Targets of Arrhythmias Induced By Ouabain and Aconitine in Guinea Pig and Rat Ventricular Myocytes]. Yao Xue Xue Bao. 2004;39(5):328-32. PubMed PMID: 15338872.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [The ion targets of arrhythmias induced by ouabain and aconitine in guinea pig and rat ventricular myocytes]. AU - Gong,Dong-mei, AU - Shan,Hong-li, AU - Zhou,Yu-hong, AU - Dong,De-li, AU - Yang,Bao-feng, PY - 2004/9/2/pubmed PY - 2004/12/16/medline PY - 2004/9/2/entrez SP - 328 EP - 32 JF - Yao xue xue bao = Acta pharmaceutica Sinica JO - Yao Xue Xue Bao VL - 39 IS - 5 N2 - AIM: To observe the effects of ouabain and aconitine on APD and ion channels in isolated guinea pig and rat ventricular myocytes; to elucidate the action mechanisms of these two drugs and set up new arrhythmic models on cellular level. METHODS: In isolated ventricular myocytes of guinea pig and rat, the effects of ouabain and aconitine on APD, ICa-L, Ik, Ito and Ik1 were observed using the whole cell patch clamp technique. RESULTS: Ouabain (5 micromol x L(-1)) obviously prolonged the APD90, increased ICa-L, decreased Ik and Ik1 in guinea pig ventricular myocytes. Aconitine (1 micromol x L(-1)) lengthened the APD90, increased ICa-L, decreased Ito and increased Ik1 in rat ventricular myocytes. CONCLUSION: The targets on ouabain- and aconitine-induced arrhythmias included APD, ICa-L, Ik, Ito, and Ik1. APD, ICaL, Ik and Ito must be the powerful ones, both in arrhythmic and antiarrhythmic courses. The ouabain- and aconitine- induced arrhythmic models on cellular level were built to study the antiarrhythmic mechanisms of chemicals and evaluate new drugs. These two new-type models in vitro were stable, liable, repeatable and economic, which were superior to those typical models in vivo. SN - 0513-4870 UR - https://www.unboundmedicine.com/medline/citation/15338872/[The_ion_targets_of_arrhythmias_induced_by_ouabain_and_aconitine_in_guinea_pig_and_rat_ventricular_myocytes]_ DB - PRIME DP - Unbound Medicine ER -