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Structural heterogeneity of cryotrapped intermediates in the bacterial blue light photoreceptor, photoactive yellow protein.
Photochem Photobiol. 2004 Jul-Aug; 80:7-14.PP

Abstract

We investigate by X-ray crystallographic techniques the cryotrapped states that accumulate on controlled illumination of the blue light photoreceptor, photoactive yellow protein (PYP), at 110 K in both the wild-type species and its E46Q mutant. These states are related to those that occur during the chromophore isomerization process in the PYP photocycle at room temperature. The structures present in such states were determined at high resolution, 0.95-1.05A. In both wild type and mutant PYP, the cryotrapped state is not composed of a single, quasitransition state structure but rather of a heterogeneous mixture of three species in addition to the ground state structure. We identify and refine these three photoactivated species under the assumption that the structural changes are limited to simple isomerization events of the chromophore that otherwise retains chemical bonding similar to that in the ground state. The refined chromophore models are essentially identical in the wild type and the E46Q mutant, which implies that the early stages of their photocycle mechanisms are the same.

Authors+Show Affiliations

Department of Biochemistry and Molecular Biology, and Consortium for Advanced Radiation Sciences, University of Chicago, Argonne National Laboratory, 9700 South Cass Avenue, Argonne, IL 60439, USA. smander@midway.uchicago.eduNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

15339224

Citation

Anderson, Spencer, et al. "Structural Heterogeneity of Cryotrapped Intermediates in the Bacterial Blue Light Photoreceptor, Photoactive Yellow Protein." Photochemistry and Photobiology, vol. 80, 2004, pp. 7-14.
Anderson S, Srajer V, Moffat K. Structural heterogeneity of cryotrapped intermediates in the bacterial blue light photoreceptor, photoactive yellow protein. Photochem Photobiol. 2004;80:7-14.
Anderson, S., Srajer, V., & Moffat, K. (2004). Structural heterogeneity of cryotrapped intermediates in the bacterial blue light photoreceptor, photoactive yellow protein. Photochemistry and Photobiology, 80, 7-14.
Anderson S, Srajer V, Moffat K. Structural Heterogeneity of Cryotrapped Intermediates in the Bacterial Blue Light Photoreceptor, Photoactive Yellow Protein. Photochem Photobiol. 2004 Jul-Aug;80:7-14. PubMed PMID: 15339224.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Structural heterogeneity of cryotrapped intermediates in the bacterial blue light photoreceptor, photoactive yellow protein. AU - Anderson,Spencer, AU - Srajer,Vukica, AU - Moffat,Keith, PY - 2004/9/2/pubmed PY - 2004/12/18/medline PY - 2004/9/2/entrez SP - 7 EP - 14 JF - Photochemistry and photobiology JO - Photochem Photobiol VL - 80 N2 - We investigate by X-ray crystallographic techniques the cryotrapped states that accumulate on controlled illumination of the blue light photoreceptor, photoactive yellow protein (PYP), at 110 K in both the wild-type species and its E46Q mutant. These states are related to those that occur during the chromophore isomerization process in the PYP photocycle at room temperature. The structures present in such states were determined at high resolution, 0.95-1.05A. In both wild type and mutant PYP, the cryotrapped state is not composed of a single, quasitransition state structure but rather of a heterogeneous mixture of three species in addition to the ground state structure. We identify and refine these three photoactivated species under the assumption that the structural changes are limited to simple isomerization events of the chromophore that otherwise retains chemical bonding similar to that in the ground state. The refined chromophore models are essentially identical in the wild type and the E46Q mutant, which implies that the early stages of their photocycle mechanisms are the same. SN - 0031-8655 UR - https://www.unboundmedicine.com/medline/citation/15339224/Structural_heterogeneity_of_cryotrapped_intermediates_in_the_bacterial_blue_light_photoreceptor_photoactive_yellow_protein_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0031-8655&date=2004&volume=80&issue=&spage=7 DB - PRIME DP - Unbound Medicine ER -