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[Isolation and identification of SARS virus in Guangdong province].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2003 Sep; 17(3):213-6.ZS

Abstract

BACKGROUND

To isolate and identify pathogen of atypical pneumonia in Guangdong.

METHODS

Pathogens were isolated from variety of samples collected from atypical pneumonia patient by using MDCK cells, and identified with serological and molecular methods.

RESULTS

A novel coronavirus was isolated from patients with atypical pneumonia, from which an RNA fragment of 279 nt was amplified by nested RT-PCR. And sequence assay showed that only 39-65 percent of sequence of the virus was homogenous to known coronavirus, but almost 100% homogenous (with one base exception, 12a to t) to SARS-associated coronavirus isolated from patients outside Guangdong, such as in Beijing, Hong Kong, Taiwan, Germany, Italy and so on. Indirect immunofluorescence test showed a specific antigen-antibody reactivity between the coronavirus and convalescent-phase sera of SARS patients.

CONCLUSION

The pathogen of the atypical pneumonia in Guangdong province was a novel type of coronavirus, which could be isolated by using MDCK cells.

Authors+Show Affiliations

Yan XG
Center for Diseases Control and Prevention of Guangdong province, Guangzhou 510300, China.
Wan ZY
No affiliation info available
Zhang X
No affiliation info available
Zheng QX
No affiliation info available
Zheng K
No affiliation info available
Huang JC
No affiliation info available
Huang P
No affiliation info available
Lu JH
No affiliation info available

MeSH

AnimalsBase SequenceCell LineChinaDogsHumansMolecular Sequence DataPhylogenyPneumonia, ViralSevere acute respiratory syndrome-related coronavirusSevere Acute Respiratory Syndrome

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

15340560

Citation

Yan, Xin-ge, et al. "[Isolation and Identification of SARS Virus in Guangdong Province]." Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi = Zhonghua Shiyan He Linchuang Bingduxue Zazhi = Chinese Journal of Experimental and Clinical Virology, vol. 17, no. 3, 2003, pp. 213-6.
Yan XG, Wan ZY, Zhang X, et al. [Isolation and identification of SARS virus in Guangdong province]. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2003;17(3):213-6.
Yan, X. G., Wan, Z. Y., Zhang, X., Zheng, Q. X., Zheng, K., Huang, J. C., Huang, P., & Lu, J. H. (2003). [Isolation and identification of SARS virus in Guangdong province]. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi = Zhonghua Shiyan He Linchuang Bingduxue Zazhi = Chinese Journal of Experimental and Clinical Virology, 17(3), 213-6.
Yan XG, et al. [Isolation and Identification of SARS Virus in Guangdong Province]. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2003;17(3):213-6. PubMed PMID: 15340560.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Isolation and identification of SARS virus in Guangdong province]. AU - Yan,Xin-ge, AU - Wan,Zhou-yue, AU - Zhang,Xin, AU - Zheng,Qiu-xia, AU - Zheng,Kui, AU - Huang,Ji-cheng, AU - Huang,Ping, AU - Lu,Jia-hai, PY - 2004/9/2/pubmed PY - 2009/4/29/medline PY - 2004/9/2/entrez SP - 213 EP - 6 JF - Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology JO - Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi VL - 17 IS - 3 N2 - BACKGROUND: To isolate and identify pathogen of atypical pneumonia in Guangdong. METHODS: Pathogens were isolated from variety of samples collected from atypical pneumonia patient by using MDCK cells, and identified with serological and molecular methods. RESULTS: A novel coronavirus was isolated from patients with atypical pneumonia, from which an RNA fragment of 279 nt was amplified by nested RT-PCR. And sequence assay showed that only 39-65 percent of sequence of the virus was homogenous to known coronavirus, but almost 100% homogenous (with one base exception, 12a to t) to SARS-associated coronavirus isolated from patients outside Guangdong, such as in Beijing, Hong Kong, Taiwan, Germany, Italy and so on. Indirect immunofluorescence test showed a specific antigen-antibody reactivity between the coronavirus and convalescent-phase sera of SARS patients. CONCLUSION: The pathogen of the atypical pneumonia in Guangdong province was a novel type of coronavirus, which could be isolated by using MDCK cells. SN - 1003-9279 UR - https://www.unboundmedicine.com/medline/citation/15340560/[Isolation_and_identification_of_SARS_virus_in_Guangdong_province]_ DB - PRIME DP - Unbound Medicine ER -