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PCR diagnosis of Pneumocystis carinii on sputum and bronchoalveolar lavage samples in immuno-compromised patients.
Parasitol Res. 2004 Oct; 94(3):213-8.PR

Abstract

The polymerase chain reaction (PCR) technique was compared with Wright-Giemsa (WG), Gomori methenamine silver (GMS) stains and an immunofluorescence assay (IFA) for detection of Pneumocystis carinii in immuno-compromised patients. Specimens of 21 bronchoalveolar lavages (BAL) and 139 sputum samples, were obtained from 157 patients (38 with AIDS and 119 with HIV) from four hospitals in Khon Kaen, Thailand. A true positive required at least two positives by techniques considered gold standard tests. Eleven (52.38%) BAL and 13 (9.35%) sputum specimens were positive. PCR produced the highest sensitivity and negative predictive values for the BAL (100% for each) vs. sputum samples at 84.62 and 98.41 percent, respectively. The specificity of PCR was 90% and 98.41% for BAL and sputum samples, respectively. We suggest PCR is an important tool for the epidemiological study of P. carinii in high-risk individuals.

Authors+Show Affiliations

Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. psomec@kku.ac.thNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15340838

Citation

Pinlaor, Somchai, et al. "PCR Diagnosis of Pneumocystis Carinii On Sputum and Bronchoalveolar Lavage Samples in Immuno-compromised Patients." Parasitology Research, vol. 94, no. 3, 2004, pp. 213-8.
Pinlaor S, Mootsikapun P, Pinlaor P, et al. PCR diagnosis of Pneumocystis carinii on sputum and bronchoalveolar lavage samples in immuno-compromised patients. Parasitol Res. 2004;94(3):213-8.
Pinlaor, S., Mootsikapun, P., Pinlaor, P., Phunmanee, A., Pipitgool, V., Sithithaworn, P., Chumpia, W., & Sithithaworn, J. (2004). PCR diagnosis of Pneumocystis carinii on sputum and bronchoalveolar lavage samples in immuno-compromised patients. Parasitology Research, 94(3), 213-8.
Pinlaor S, et al. PCR Diagnosis of Pneumocystis Carinii On Sputum and Bronchoalveolar Lavage Samples in Immuno-compromised Patients. Parasitol Res. 2004;94(3):213-8. PubMed PMID: 15340838.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - PCR diagnosis of Pneumocystis carinii on sputum and bronchoalveolar lavage samples in immuno-compromised patients. AU - Pinlaor,Somchai, AU - Mootsikapun,Piroon, AU - Pinlaor,Porntip, AU - Phunmanee,Anakapong, AU - Pipitgool,Vichit, AU - Sithithaworn,Paiboon, AU - Chumpia,Worawan, AU - Sithithaworn,Jiraporn, Y1 - 2004/09/01/ PY - 2004/05/12/received PY - 2004/07/12/accepted PY - 2004/9/2/pubmed PY - 2004/12/16/medline PY - 2004/9/2/entrez SP - 213 EP - 8 JF - Parasitology research JO - Parasitol Res VL - 94 IS - 3 N2 - The polymerase chain reaction (PCR) technique was compared with Wright-Giemsa (WG), Gomori methenamine silver (GMS) stains and an immunofluorescence assay (IFA) for detection of Pneumocystis carinii in immuno-compromised patients. Specimens of 21 bronchoalveolar lavages (BAL) and 139 sputum samples, were obtained from 157 patients (38 with AIDS and 119 with HIV) from four hospitals in Khon Kaen, Thailand. A true positive required at least two positives by techniques considered gold standard tests. Eleven (52.38%) BAL and 13 (9.35%) sputum specimens were positive. PCR produced the highest sensitivity and negative predictive values for the BAL (100% for each) vs. sputum samples at 84.62 and 98.41 percent, respectively. The specificity of PCR was 90% and 98.41% for BAL and sputum samples, respectively. We suggest PCR is an important tool for the epidemiological study of P. carinii in high-risk individuals. SN - 0932-0113 UR - https://www.unboundmedicine.com/medline/citation/15340838/PCR_diagnosis_of_Pneumocystis_carinii_on_sputum_and_bronchoalveolar_lavage_samples_in_immuno_compromised_patients_ DB - PRIME DP - Unbound Medicine ER -