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Subminute and sensitive determination of the neurotransmitter serotonin in urine by capillary electrophoresis with laser-induced fluorescence detection.
Biomed Chromatogr. 2004 Sep; 18(7):422-6.BC

Abstract

In this work, a sub-minute and sensitive capillary electrophoresis with laser-induced fluorescence (CE-LIF) method was developed for the analysis and quantitation of the neurotransmitter 5-hydroxytryptamine (5-HT) or serotonin in urine. The method involves precolumn derivatization with fluorescein isothiocyanate isomer I (FITC) using an excitation light from an argon ion laser of 488 nm and a 520 nm band pass emission filter. Different variables that affect derivatization (pH, FITC concentration, reaction time and temperature) and separation (buffer concentration, pH, applied voltage and injection time) were studied. The linear dynamic range obtained was between 0 and 188 nM with a detection limit of 16 nm with a RSD between 2 and 9%. The applicability of the proposed method was demonstrated by analysis of 5-HT in human urine, establishing a concentration of 57 nM in control urine. The method was validated by standard-addition methodology.

Authors+Show Affiliations

Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Spain.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15340966

Citation

Román, David Arráez, et al. "Subminute and Sensitive Determination of the Neurotransmitter Serotonin in Urine By Capillary Electrophoresis With Laser-induced Fluorescence Detection." Biomedical Chromatography : BMC, vol. 18, no. 7, 2004, pp. 422-6.
Román DA, Carretero AS, Blanco CC, et al. Subminute and sensitive determination of the neurotransmitter serotonin in urine by capillary electrophoresis with laser-induced fluorescence detection. Biomed Chromatogr. 2004;18(7):422-6.
Román, D. A., Carretero, A. S., Blanco, C. C., & Gutiérrez, A. F. (2004). Subminute and sensitive determination of the neurotransmitter serotonin in urine by capillary electrophoresis with laser-induced fluorescence detection. Biomedical Chromatography : BMC, 18(7), 422-6.
Román DA, et al. Subminute and Sensitive Determination of the Neurotransmitter Serotonin in Urine By Capillary Electrophoresis With Laser-induced Fluorescence Detection. Biomed Chromatogr. 2004;18(7):422-6. PubMed PMID: 15340966.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Subminute and sensitive determination of the neurotransmitter serotonin in urine by capillary electrophoresis with laser-induced fluorescence detection. AU - Román,David Arráez, AU - Carretero,Antonio Segura, AU - Blanco,Carmen Cruces, AU - Gutiérrez,Alberto Fernández, PY - 2004/9/2/pubmed PY - 2004/12/16/medline PY - 2004/9/2/entrez SP - 422 EP - 6 JF - Biomedical chromatography : BMC JO - Biomed Chromatogr VL - 18 IS - 7 N2 - In this work, a sub-minute and sensitive capillary electrophoresis with laser-induced fluorescence (CE-LIF) method was developed for the analysis and quantitation of the neurotransmitter 5-hydroxytryptamine (5-HT) or serotonin in urine. The method involves precolumn derivatization with fluorescein isothiocyanate isomer I (FITC) using an excitation light from an argon ion laser of 488 nm and a 520 nm band pass emission filter. Different variables that affect derivatization (pH, FITC concentration, reaction time and temperature) and separation (buffer concentration, pH, applied voltage and injection time) were studied. The linear dynamic range obtained was between 0 and 188 nM with a detection limit of 16 nm with a RSD between 2 and 9%. The applicability of the proposed method was demonstrated by analysis of 5-HT in human urine, establishing a concentration of 57 nM in control urine. The method was validated by standard-addition methodology. SN - 0269-3879 UR - https://www.unboundmedicine.com/medline/citation/15340966/Subminute_and_sensitive_determination_of_the_neurotransmitter_serotonin_in_urine_by_capillary_electrophoresis_with_laser_induced_fluorescence_detection_ L2 - https://doi.org/10.1002/bmc.330 DB - PRIME DP - Unbound Medicine ER -