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Inactivation of endopolyphosphatase gene PPN1 results in inhibition of expression of exopolyphosphatase PPX1 and high-molecular-mass exopolyphosphatase not encoded by PPX1 in Saccharomyces cerevisiae.
Biochim Biophys Acta. 2004 Sep 06; 1674(1):98-102.BB

Abstract

Saccharomyces cerevisiae possesses multiple forms of exopolyphosphatases, the enzymes involved in the metabolism of inorganic polyphosphates, which are important regulatory compounds. In S. cerevisiae, inactivation of endopolyphosphatase gene PPN1 leads to the inhibition of expression of both exopolyphosphatase PPX1 and high-molecular-mass exopolyphosphatase of approximately 1000 kDa not encoded by PPX1. In the single endopolyphosphatase mutant CRN, the expression of exopolyphosphatase PPX1 decreases 6.5-fold and 2.5-fold at the stationary and exponential growth phases, respectively, as compared with the parent strain CRY. In this mutant, the activity of the high-molecular-mass exopolyphosphatase of approximately 1000 kDa decreases approximately 10-fold as compared with that in strains with the PPN1 gene. In a double mutant of PPX1 and PPN1, no exopolyphosphatase activity is detected in the cytosol at the stationary growth phase. Thus, the exopolyPase activity in cell cytosol depends on the endopolyPase gene PPN1.

Authors+Show Affiliations

Lichko L
Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Pushchino, Moscow Region 142290, Russia.
Kulakovskaya T
No affiliation info available
Kulaev I
No affiliation info available

MeSH

Acid Anhydride HydrolasesCell SizeGene Expression Regulation, FungalGene SilencingMolecular WeightPhenotypeSaccharomyces cerevisiaeSaccharomyces cerevisiae Proteins

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

15342119

Citation

Lichko, Lidiya, et al. "Inactivation of Endopolyphosphatase Gene PPN1 Results in Inhibition of Expression of Exopolyphosphatase PPX1 and High-molecular-mass Exopolyphosphatase Not Encoded By PPX1 in Saccharomyces Cerevisiae." Biochimica Et Biophysica Acta, vol. 1674, no. 1, 2004, pp. 98-102.
Lichko L, Kulakovskaya T, Kulaev I. Inactivation of endopolyphosphatase gene PPN1 results in inhibition of expression of exopolyphosphatase PPX1 and high-molecular-mass exopolyphosphatase not encoded by PPX1 in Saccharomyces cerevisiae. Biochim Biophys Acta. 2004;1674(1):98-102.
Lichko, L., Kulakovskaya, T., & Kulaev, I. (2004). Inactivation of endopolyphosphatase gene PPN1 results in inhibition of expression of exopolyphosphatase PPX1 and high-molecular-mass exopolyphosphatase not encoded by PPX1 in Saccharomyces cerevisiae. Biochimica Et Biophysica Acta, 1674(1), 98-102.
Lichko L, Kulakovskaya T, Kulaev I. Inactivation of Endopolyphosphatase Gene PPN1 Results in Inhibition of Expression of Exopolyphosphatase PPX1 and High-molecular-mass Exopolyphosphatase Not Encoded By PPX1 in Saccharomyces Cerevisiae. Biochim Biophys Acta. 2004 Sep 6;1674(1):98-102. PubMed PMID: 15342119.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inactivation of endopolyphosphatase gene PPN1 results in inhibition of expression of exopolyphosphatase PPX1 and high-molecular-mass exopolyphosphatase not encoded by PPX1 in Saccharomyces cerevisiae. AU - Lichko,Lidiya, AU - Kulakovskaya,Tatyana, AU - Kulaev,Igor, PY - 2004/04/26/received PY - 2004/05/28/revised PY - 2004/06/04/accepted PY - 2004/9/3/pubmed PY - 2004/11/2/medline PY - 2004/9/3/entrez SP - 98 EP - 102 JF - Biochimica et biophysica acta JO - Biochim Biophys Acta VL - 1674 IS - 1 N2 - Saccharomyces cerevisiae possesses multiple forms of exopolyphosphatases, the enzymes involved in the metabolism of inorganic polyphosphates, which are important regulatory compounds. In S. cerevisiae, inactivation of endopolyphosphatase gene PPN1 leads to the inhibition of expression of both exopolyphosphatase PPX1 and high-molecular-mass exopolyphosphatase of approximately 1000 kDa not encoded by PPX1. In the single endopolyphosphatase mutant CRN, the expression of exopolyphosphatase PPX1 decreases 6.5-fold and 2.5-fold at the stationary and exponential growth phases, respectively, as compared with the parent strain CRY. In this mutant, the activity of the high-molecular-mass exopolyphosphatase of approximately 1000 kDa decreases approximately 10-fold as compared with that in strains with the PPN1 gene. In a double mutant of PPX1 and PPN1, no exopolyphosphatase activity is detected in the cytosol at the stationary growth phase. Thus, the exopolyPase activity in cell cytosol depends on the endopolyPase gene PPN1. SN - 0006-3002 UR - https://www.unboundmedicine.com/medline/citation/15342119/Inactivation_of_endopolyphosphatase_gene_PPN1_results_in_inhibition_of_expression_of_exopolyphosphatase_PPX1_and_high_molecular_mass_exopolyphosphatase_not_encoded_by_PPX1_in_Saccharomyces_cerevisiae_ DB - PRIME DP - Unbound Medicine ER -